MPCP3_ARATH
ID MPCP3_ARATH Reviewed; 375 AA.
AC Q9FMU6; O80415; Q56WI0;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 132.
DE RecName: Full=Mitochondrial phosphate carrier protein 3, mitochondrial;
DE AltName: Full=Mitochondrial phosphate transporter 3;
DE Short=MPT3;
DE AltName: Full=Phosphate transporter 3;1;
DE Flags: Precursor;
GN Name=MPT3; Synonyms=AT5, PHT3;1; OrderedLocusNames=At5g14040;
GN ORFNames=MUA22_4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 88-375.
RC STRAIN=cv. Columbia;
RX PubMed=10437831; DOI=10.1023/a:1006285009435;
RA Takabatake R., Hata S., Taniguchi M., Kouchi H., Sugiyama T., Izui K.;
RT "Isolation and characterization of cDNAs encoding mitochondrial phosphate
RT transporters in soybean, maize, rice, and Arabidopis.";
RL Plant Mol. Biol. 40:479-486(1999).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 220-375.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=14756774; DOI=10.1046/j.1365-2958.2003.03810.x;
RA Hamel P., Saint-Georges Y., de Pinto B., Lachacinski N., Altamura N.,
RA Dujardin G.;
RT "Redundancy in the function of mitochondrial phosphate transport in
RT Saccharomyces cerevisiae and Arabidopsis thaliana.";
RL Mol. Microbiol. 51:307-317(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=14671022; DOI=10.1105/tpc.016055;
RA Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J.,
RA Millar A.H.;
RT "Experimental analysis of the Arabidopsis mitochondrial proteome highlights
RT signaling and regulatory components, provides assessment of targeting
RT prediction programs, and indicates plant-specific mitochondrial proteins.";
RL Plant Cell 16:241-256(2004).
RN [8]
RP GENE FAMILY.
RX PubMed=15003237; DOI=10.1016/j.tplants.2004.01.007;
RA Picault N., Hodges M., Palmieri L., Palmieri F.;
RT "The growing family of mitochondrial carriers in Arabidopsis.";
RL Trends Plant Sci. 9:138-146(2004).
RN [9]
RP GENE FAMILY, TISSUE SPECIFICITY, INDUCTION BY SALT, AND FUNCTION.
RX PubMed=22937061; DOI=10.1371/journal.pone.0043530;
RA Zhu W., Miao Q., Sun D., Yang G., Wu C., Huang J., Zheng C.;
RT "The mitochondrial phosphate transporters modulate plant responses to salt
RT stress via affecting ATP and gibberellin metabolism in Arabidopsis
RT thaliana.";
RL PLoS ONE 7:E43530-E43530(2012).
CC -!- FUNCTION: Transport of phosphate groups from the cytosol to the
CC mitochondrial matrix. Mediates salt stress tolerance through an ATP-
CC dependent pathway and via modulation of the gibberellin metabolism.
CC {ECO:0000269|PubMed:14756774, ECO:0000269|PubMed:22937061}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:14671022}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:14671022}.
CC -!- TISSUE SPECIFICITY: Expressed in stems, leaves and flowers. Strong
CC expression in vascular tissues. {ECO:0000269|PubMed:22937061}.
CC -!- INDUCTION: By salt stress. {ECO:0000269|PubMed:22937061}.
CC -!- MISCELLANEOUS: Plants overexpressing MPT3/PHT3;1 display increased
CC sensitivity to salt stress. {ECO:0000305|PubMed:22937061}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD94852.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB007650; BAB08283.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91980.1; -; Genomic_DNA.
DR EMBL; AY058848; AAL24236.1; -; mRNA.
DR EMBL; AY143869; AAN28808.1; -; mRNA.
DR EMBL; AB016066; BAA31585.1; -; mRNA.
DR EMBL; AK222060; BAD94852.1; ALT_INIT; mRNA.
DR PIR; T51595; T51595.
DR RefSeq; NP_196908.1; NM_121407.3.
DR AlphaFoldDB; Q9FMU6; -.
DR SMR; Q9FMU6; -.
DR BioGRID; 16530; 24.
DR IntAct; Q9FMU6; 1.
DR MINT; Q9FMU6; -.
DR STRING; 3702.AT5G14040.1; -.
DR TCDB; 2.A.29.4.6; the mitochondrial carrier (mc) family.
DR PaxDb; Q9FMU6; -.
DR PRIDE; Q9FMU6; -.
DR ProteomicsDB; 239065; -.
DR EnsemblPlants; AT5G14040.1; AT5G14040.1; AT5G14040.
DR GeneID; 831252; -.
DR Gramene; AT5G14040.1; AT5G14040.1; AT5G14040.
DR KEGG; ath:AT5G14040; -.
DR Araport; AT5G14040; -.
DR TAIR; locus:2174688; AT5G14040.
DR eggNOG; KOG0767; Eukaryota.
DR HOGENOM; CLU_039456_0_1_1; -.
DR InParanoid; Q9FMU6; -.
DR OMA; MISRKNF; -.
DR OrthoDB; 963446at2759; -.
DR PhylomeDB; Q9FMU6; -.
DR BRENDA; 7.3.2.1; 399.
DR PRO; PR:Q9FMU6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FMU6; baseline and differential.
DR Genevisible; Q9FMU6; AT.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0009505; C:plant-type cell wall; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:1990547; P:mitochondrial phosphate ion transmembrane transport; IEA:InterPro.
DR GO; GO:0035435; P:phosphate ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0009651; P:response to salt stress; IMP:TAIR.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR InterPro; IPR044677; Pic2/Mir1-like.
DR PANTHER; PTHR45671; PTHR45671; 1.
DR Pfam; PF00153; Mito_carr; 2.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..375
FT /note="Mitochondrial phosphate carrier protein 3,
FT mitochondrial"
FT /id="PRO_0000421697"
FT TOPO_DOM ?..75
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 97..134
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..154
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..175
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..231
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..251
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 252..272
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 294..332
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 354..375
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT REPEAT 76..160
FT /note="Solcar 1"
FT REPEAT 173..257
FT /note="Solcar 2"
FT REPEAT 274..353
FT /note="Solcar 3"
SQ SEQUENCE 375 AA; 40090 MW; C58B3057954C9CF6 CRC64;
MESPKNSLIP SFLYSSSSSP RSFLLDQVLN SNSNAAFEKS PSPAPRSSPT SMISRKNFLI
ASPTEPGKGI EMYSPAFYAA CTFGGILSCG LTHMTVTPLD LVKCNMQIDP AKYKSISSGF
GILLKEQGVK GFFRGWVPTL LGYSAQGACK FGFYEYFKKT YSDLAGPEYT AKYKTLIYLA
GSASAEIIAD IALCPFEAVK VRVQTQPGFA RGMSDGFPKF IKSEGYGGLY KGLAPLWGRQ
IPYTMMKFAS FETIVEMIYK YAIPNPKSEC SKGLQLGVSF AGGYVAGVFC AIVSHPADNL
VSFLNNAKGA TVGDAVKKIG MVGLFTRGLP LRIVMIGTLT GAQWGLYDAF KVFVGLPTTG
GVAPAPAIAA TEAKA
