MPDC_MYCAO
ID MPDC_MYCAO Reviewed; 504 AA.
AC Q3YAT5;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Hydroxyisobutyraldehyde dehydrogenase {ECO:0000303|PubMed:16622053};
DE EC=1.2.1.98 {ECO:0000269|PubMed:16622053};
GN Name=mpdC {ECO:0000303|PubMed:16622053};
OS Mycolicibacterium austroafricanum (Mycobacterium austroafricanum).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=39687;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY,
RP SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=IFP 2012;
RX PubMed=16622053; DOI=10.1099/mic.0.28585-0;
RA Lopes Ferreira N., Labbe D., Monot F., Fayolle-Guichard F., Greer C.W.;
RT "Genes involved in the methyl tert-butyl ether (MTBE) metabolic pathway of
RT Mycobacterium austroafricanum IFP 2012.";
RL Microbiology 152:1361-1374(2006).
CC -!- FUNCTION: Involved in the degradation of methyl tert-butyl ether
CC (MTBE). Catalyzes the conversion of hydroxyisobutyraldehyde to
CC hydroxyisobutyric acid (HIBA). {ECO:0000269|PubMed:16622053}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-hydroxy-2-methylpropanal + H2O + NAD(+) = 2-hydroxy-2-
CC methylpropanoate + 2 H(+) + NADH; Xref=Rhea:RHEA:49616,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:19641,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:131846;
CC EC=1.2.1.98; Evidence={ECO:0000269|PubMed:16622053};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16622053}.
CC -!- INDUCTION: Induced in the presence of MTBE or tert-butyl alcohol (TBA).
CC {ECO:0000269|PubMed:16622053}.
CC -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC {ECO:0000305}.
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DR EMBL; DQ147773; AAZ78235.1; -; Genomic_DNA.
DR AlphaFoldDB; Q3YAT5; -.
DR SMR; Q3YAT5; -.
DR KEGG; ag:AAZ78235; -.
DR BioCyc; MetaCyc:MON-19854; -.
DR BRENDA; 1.2.1.98; 14567.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016620; F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR Gene3D; 3.40.309.10; -; 1.
DR Gene3D; 3.40.605.10; -; 1.
DR InterPro; IPR016161; Ald_DH/histidinol_DH.
DR InterPro; IPR016163; Ald_DH_C.
DR InterPro; IPR029510; Ald_DH_CS_GLU.
DR InterPro; IPR016162; Ald_DH_N.
DR InterPro; IPR015590; Aldehyde_DH_dom.
DR Pfam; PF00171; Aldedh; 1.
DR SUPFAM; SSF53720; SSF53720; 1.
DR PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; NAD; Oxidoreductase.
FT CHAIN 1..504
FT /note="Hydroxyisobutyraldehyde dehydrogenase"
FT /id="PRO_0000447201"
FT ACT_SITE 260
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:P25526"
FT ACT_SITE 294
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:P25526"
SQ SEQUENCE 504 AA; 53848 MW; E3DFCF0822C37C09 CRC64;
MTRTLSADAD TRTATPPLMY VNGEWLPARS GATFPTIEPS TGRPITEIPR GDSSDVDAAV
KAAADVAVEW QFTDAITRAA LLRRLAELVA ENAEELARIE SLDSGHYLAK ARELVTAIPL
WLEYWAGAAD KVGGRTIAVP GNKLSFTLLE PLGVTAHIIP WNYPLLILAR SIAPALALGN
TCVVKPAEDT SLSALKFAEL VHAAGFPAGV FNVVTGYGSE AGAALAAHPE VRGITFTGST
ETGREIARLG GQHIAQVNLE LGGKSPLVVF PDAPLEDAVE VAVQGFCSRA GQVCVAGSRL
FLHEDIADRF LEMLVSRLET VTVGDPFDGA TQMGPLASKK HYDRVREYIE VGKQEATLLY
GGGRPTDTPD DGFFVEPTVF VDVATDARIA REEIFGPVTA VMRWSSVDDL IATINDSEFG
LFAVLWCRDI TSALDTAKRL QVGSVMINDW FGELPMTPHG GHKQSGTGRE EGLEAVHGYT
QVKHIGINLE PSPAKSADWA GAPL