ID   MPG1_CRYNJ              Reviewed;         364 AA.
AC   P0CO20; Q45T76; Q55VR7; Q5KKH2; Q5WQW2;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Mannose-1-phosphate guanyltransferase;
DE            EC=2.7.7.13;
DE   AltName: Full=GDP-mannose pyrophosphorylase;
DE   AltName: Full=GTP-mannose-1-phosphate guanylyltransferase;
GN   Name=MPG1; Synonyms=PSA1; OrderedLocusNames=CNC03020;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Janbon G.;
RT   "PSA1 encodes a putative GDP-mannose pyrophosphorylase.";
RL   Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 7-358.
RC   STRAIN=B4500;
RA   Warit S., Palittapongarnpim P.;
RT   "Cloning and characterization of SRB1/PSA1 homolog of Cryptococcus
RT   neoformans.";
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in cell wall synthesis where it is required for
CC       glycosylation. Involved in cell cycle progression through cell-size
CC       checkpoint (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-mannose 1-phosphate + GTP + H(+) = diphosphate + GDP-
CC         alpha-D-mannose; Xref=Rhea:RHEA:15229, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:37565, ChEBI:CHEBI:57527,
CC         ChEBI:CHEBI:58409; EC=2.7.7.13;
CC   -!- PATHWAY: Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose
CC       biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP
CC       route): step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the transferase hexapeptide repeat family.
CC       {ECO:0000305}.
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DR   EMBL; AY426181; AAR84601.1; -; Genomic_DNA.
DR   EMBL; AE017343; AAW42293.1; -; Genomic_DNA.
DR   EMBL; DQ116945; AAZ22401.1; -; mRNA.
DR   RefSeq; XP_569600.1; XM_569600.1.
DR   AlphaFoldDB; P0CO20; -.
DR   SMR; P0CO20; -.
DR   STRING; 5207.AAW42293; -.
DR   PaxDb; P0CO20; -.
DR   EnsemblFungi; AAW42293; AAW42293; CNC03020.
DR   GeneID; 3256248; -.
DR   KEGG; cne:CNC03020; -.
DR   VEuPathDB; FungiDB:CNC03020; -.
DR   eggNOG; KOG1322; Eukaryota.
DR   HOGENOM; CLU_029499_0_0_1; -.
DR   InParanoid; P0CO20; -.
DR   OMA; PFLTHQL; -.
DR   OrthoDB; 806744at2759; -.
DR   UniPathway; UPA00126; UER00930.
DR   Proteomes; UP000002149; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004475; F:mannose-1-phosphate guanylyltransferase activity; IBA:GO_Central.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IBA:GO_Central.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0000032; P:cell wall mannoprotein biosynthetic process; IBA:GO_Central.
DR   GO; GO:0009298; P:GDP-mannose biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006486; P:protein glycosylation; IBA:GO_Central.
DR   CDD; cd06425; M1P_guanylylT_B_like_N; 1.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR045233; GMPPB_N.
DR   InterPro; IPR001451; Hexapep.
DR   InterPro; IPR018357; Hexapep_transf_CS.
DR   InterPro; IPR005835; NTP_transferase_dom.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00132; Hexapep; 1.
DR   Pfam; PF00483; NTP_transferase; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
DR   PROSITE; PS00101; HEXAPEP_TRANSFERASES; 2.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cytoplasm; GTP-binding; Nucleotide-binding;
KW   Nucleotidyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..364
FT                   /note="Mannose-1-phosphate guanyltransferase"
FT                   /id="PRO_0000238486"
FT   CONFLICT        1..2
FT                   /note="MK -> MPCPTRPYNEDKCAPTARSRRLTRSAPH (in Ref. 1;
FT                   AAR84601)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   364 AA;  40063 MW;  EE99B9502A0F925C CRC64;
     MKALILVGGF GTRLRPLTLS WPKPLVEFCN KAMILHQIEA LVKAGVKDIV LAVNYRPEVM
     VSVLKKTEEE FGINIHFSVE TEPLGTAGPL ALAREILGKD DSPFFVLNSD VTCVYPFEAF
     RDFHIAHKCE GSIMVTKVAE PSAYGVVVTK PNSTVIDRFV EKPVEFVGNR INAGIYIFNP
     SVLDRIELRP TSIEKEIFPA IAADQQLHSF DLQGFWMDVG QPKDFLAGTC LYLSHLTSQH
     SPLLTDPSQN KWVYGGNVMV DPSAEIDPTA VIGPNVVIGP DAKIGPGVRL QRCVIMSNAT
     VRDHSWIANS IVGWNSTVGR WTRVENITVL GDDVTIKDEL YVNGASVLPH KSISTSITEP
     RIVM