ARNE_ECOLI
ID ARNE_ECOLI Reviewed; 111 AA.
AC Q47377; Q2EES8; Q2MAN1;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE;
DE Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnE;
DE AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnE;
GN Name=arnE; Synonyms=pmrL, yfbW; OrderedLocusNames=b4544, JW2252;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-111.
RC STRAIN=K12;
RX PubMed=8626063; DOI=10.1016/0378-1119(95)00721-0;
RA Sharma V., Hudspeth M.E.S., Meganathan R.;
RT "Menaquinone (vitamin K2) biosynthesis: localization and characterization
RT of the menE gene from Escherichia coli.";
RL Gene 168:43-48(1996).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [5]
RP FUNCTION, SUBUNIT, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=17928292; DOI=10.1074/jbc.m706172200;
RA Yan A., Guan Z., Raetz C.R.H.;
RT "An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin
RT resistance in Escherichia coli.";
RL J. Biol. Chem. 282:36077-36089(2007).
CC -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC periplasmic side of the inner membrane. {ECO:0000269|PubMed:17928292}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis.
CC -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000305|PubMed:17928292}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene lack L-Ara4N-modified
CC lipid A and are sensitive to polymyxin. {ECO:0000269|PubMed:17928292}.
CC -!- SIMILARITY: Belongs to the ArnE family. {ECO:0000305}.
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DR EMBL; U00096; ABD18693.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76675.1; -; Genomic_DNA.
DR EMBL; L35031; AAB04895.1; -; Genomic_DNA.
DR RefSeq; WP_000638031.1; NZ_STEB01000008.1.
DR RefSeq; YP_588462.1; NC_000913.3.
DR AlphaFoldDB; Q47377; -.
DR SMR; Q47377; -.
DR BioGRID; 4260500; 209.
DR STRING; 511145.b4544; -.
DR TCDB; 2.A.7.22.1; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; Q47377; -.
DR PRIDE; Q47377; -.
DR EnsemblBacteria; ABD18693; ABD18693; b4544.
DR EnsemblBacteria; BAE76675; BAE76675; BAE76675.
DR GeneID; 1450282; -.
DR GeneID; 66673856; -.
DR KEGG; ecj:JW2252; -.
DR KEGG; eco:b4544; -.
DR PATRIC; fig|1411691.4.peg.4479; -.
DR EchoBASE; EB4090; -.
DR eggNOG; COG2076; Bacteria.
DR HOGENOM; CLU_131462_5_1_6; -.
DR InParanoid; Q47377; -.
DR OMA; TCAGQLC; -.
DR PhylomeDB; Q47377; -.
DR BioCyc; EcoCyc:MON0-2682; -.
DR BioCyc; MetaCyc:MON0-2682; -.
DR UniPathway; UPA00030; -.
DR PRO; PR:Q47377; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:1901264; P:carbohydrate derivative transport; IMP:EcoCyc.
DR GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0010041; P:response to iron(III) ion; IEP:EcoCyc.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR HAMAP; MF_01869; Flippase_ArnE; 1.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR022883; Flippase_ArnE.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR PANTHER; PTHR30561; PTHR30561; 1.
DR Pfam; PF00892; EamA; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..111
FT /note="Probable 4-amino-4-deoxy-L-arabinose-
FT phosphoundecaprenol flippase subunit ArnE"
FT /id="PRO_0000169184"
FT TOPO_DOM 1..35
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 57..61
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..87
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..111
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 40..109
FT /note="EamA"
SQ SEQUENCE 111 AA; 12192 MW; 7CFA06D75DA33D69 CRC64;
MIWLTLVFAS LLSVAGQLCQ KQATCFVAIN KRRKHIVLWL GLALACLGLA MVLWLLVLQN
VPVGIAYPML SLNFVWVTLA AVKLWHEPVS PRHWCGVAFI IGGIVILGST V
