ID   MPI1_LYMST              Reviewed;         123 AA.
AC   P07223; Q9TWW5; Q9TWW6;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 2.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Molluscan insulin-related peptide 1;
DE            Short=MIP I;
DE   Contains:
DE     RecName: Full=Molluscan insulin-related peptide 1 B chain;
DE   Contains:
DE     RecName: Full=C-alpha peptide;
DE   Contains:
DE     RecName: Full=Molluscan insulin-related peptide 1 A chain;
DE   Flags: Precursor;
OS   Lymnaea stagnalis (Great pond snail) (Helix stagnalis).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Heterobranchia; Euthyneura; Panpulmonata; Hygrophila; Lymnaeoidea;
OC   Lymnaeidae; Lymnaea.
OX   NCBI_TaxID=6523;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=3340203; DOI=10.1038/331535a0;
RA   Smit A.B., Vreugdenhil E., Ebberink R.H.M., Geraerts W.P.M., Klootwijk J.,
RA   Joosse J.;
RT   "Growth-controlling molluscan neurons produce the precursor of an insulin-
RT   related peptide.";
RL   Nature 331:535-538(1988).
RN   [2]
RP   PROTEIN SEQUENCE OF 32-67; 72-96 AND 99-123, AND PYROGLUTAMATE FORMATION AT
RP   GLN-32 AND GLN-99.
RC   TISSUE=Light-green cell;
RX   PubMed=1526314; DOI=10.1016/0303-7207(92)90133-q;
RA   Li K.W., Geraerts W.P.M., Ebberink R.H.M., Joosse J.;
RT   "Purification and sequencing of molluscan insulin-related peptide I (MIP I)
RT   from the neuroendocrine light green cells of Lymnaea stagnalis.";
RL   Mol. Cell. Endocrinol. 85:141-150(1992).
CC   -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC       disulfide bonds.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle.
CC       Note=Secretory granules.
CC   -!- TISSUE SPECIFICITY: Expressed in the cerebral light-green cells which
CC       are giant neuroendocrines cells involved in the control of growth.
CC   -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
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DR   EMBL; X06983; CAA30043.1; -; mRNA.
DR   PIR; S00531; S00531.
DR   AlphaFoldDB; P07223; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0030133; C:transport vesicle; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:InterPro.
DR   InterPro; IPR016179; Insulin-like.
DR   InterPro; IPR036438; Insulin-like_sf.
DR   InterPro; IPR016724; Insulin-rel_pep.
DR   InterPro; IPR022353; Insulin_CS.
DR   InterPro; IPR022352; Insulin_family.
DR   Pfam; PF00049; Insulin; 1.
DR   PIRSF; PIRSF018431; Molluscan_insulin_rel_peptide; 1.
DR   PRINTS; PR00276; INSULINFAMLY.
DR   SMART; SM00078; IlGF; 1.
DR   SUPFAM; SSF56994; SSF56994; 1.
DR   PROSITE; PS00262; INSULIN; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Cytoplasmic vesicle;
KW   Direct protein sequencing; Disulfide bond; Pyrrolidone carboxylic acid;
KW   Signal.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000255"
FT   PEPTIDE         32..67
FT                   /note="Molluscan insulin-related peptide 1 B chain"
FT                   /id="PRO_0000015937"
FT   PROPEP          68..69
FT                   /id="PRO_0000015938"
FT   PEPTIDE         72..96
FT                   /note="C-alpha peptide"
FT                   /id="PRO_0000015939"
FT   PEPTIDE         99..123
FT                   /note="Molluscan insulin-related peptide 1 A chain"
FT                   /id="PRO_0000015940"
FT   MOD_RES         32
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:1526314"
FT   MOD_RES         99
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:1526314"
FT   DISULFID        48..109
FT                   /note="Interchain (between B and A chains)"
FT   DISULFID        60..122
FT                   /note="Interchain (between B and A chains)"
FT   DISULFID        108..113
SQ   SEQUENCE   123 AA;  13458 MW;  87C89F042E2D700B CRC64;
     MAGVRLVFTK AFMVTVLLTL LLNIGVKPAE GQFSACNIND RPHRRGVCGS ALADLVDFAC
     SSSNQPAMVK RNAETDLDDP LRNIKLSSES ALTYLTKRQG TTNIVCECCM KPCTLSELRQ
     YCP