MPIP2_CAEEL
ID MPIP2_CAEEL Reviewed; 480 AA.
AC O44628;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=M-phase inducer phosphatase cdc-25.2;
DE EC=3.1.3.48;
DE AltName: Full=Cell division cycle-related protein 25.2;
GN Name=cdc-25.2 {ECO:0000312|WormBase:F16B4.8a};
GN ORFNames=F16B4.8 {ECO:0000312|WormBase:F16B4.8a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP IDENTIFICATION.
RX PubMed=9651482; DOI=10.1016/s0378-1119(98)00228-5;
RA Ashcroft N.R., Kosinski M.E., Wickramasinghe D., Donovan P.J., Golden A.;
RT "The four cdc25 genes from the nematode Caenorhabditis elegans.";
RL Gene 214:59-66(1998).
RN [3]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=27104746; DOI=10.1080/15384101.2016.1146839;
RA Lee Y.U., Son M., Kim J., Shim Y.H., Kawasaki I.;
RT "CDC-25.2, a C. elegans ortholog of cdc25, is essential for the progression
RT of intestinal divisions.";
RL Cell Cycle 15:654-666(2016).
RN [4]
RP FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP SER-239.
RX PubMed=27923661; DOI=10.1016/j.bbrc.2016.12.014;
RA Oh S., Yoon S., Youn E., Kawasaki I., Shim Y.H.;
RT "cdc-25.2, a Caenorhabditis elegans ortholog of cdc25, is required for male
RT tail morphogenesis.";
RL Biochem. Biophys. Res. Commun. 482:1213-1218(2017).
CC -!- FUNCTION: Required for intestinal cell division following the 16E cell
CC stage of embryogenesis (PubMed:27104746). Regulates intestinal cell
CC divisions and binucleations probably by modulating the activity of the
CC cell cycle regulator wee-1.3 and by activating the cdk-1/cyb-1 complex
CC (PubMed:27104746). Plays a role in male tail development, via
CC regulation of the cell divisions of the ray precursor cell lineages,
CC perhaps acting together with cell cycle regulators cyl-1, cdk-1, cyb-3,
CC and cyd-1 (PubMed:27923661). {ECO:0000269|PubMed:27104746,
CC ECO:0000269|PubMed:27923661}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:10684, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620; EC=3.1.3.48;
CC -!- DEVELOPMENTAL STAGE: Transiently expressed in the intestine during
CC larval development (PubMed:27104746). Expressed in the intestine in L1
CC stage larvae, not expressed in L2 to L3 stage larvae, but is then again
CC expressed in the gonad of L4 stage larvae (PubMed:27104746). Expressed
CC in seam cells during larval development (PubMed:27923661). Broadly
CC expressed throughout development in multiple male somatic tissues,
CC including pharynx, ventral nerve cord, body muscles, diagonal muscles,
CC and hypodermis (PubMed:27923661). {ECO:0000269|PubMed:27104746,
CC ECO:0000269|PubMed:27923661}.
CC -!- DISRUPTION PHENOTYPE: Viable, but 85% of animals are sterile at 20
CC degrees Celsius (PubMed:27104746). Reduced number of intestinal cells
CC in 1.5-fold stage embryos due to defective intestinal divisions and
CC binuleations at the 16E cell stage of embryonic development
CC (PubMed:27104746). Knockout with wee-1.3 RNAi suppresses the defect in
CC intestinal cell divisions in the cdc-25.2 single mutant
CC (PubMed:27104746). RNAi-mediated knockdown causes abnormal male tail
CC morphology; this phenotype is suppressed by simultaneous RNAi-mediated
CC knockdown of wee-1.3 (PubMed:27923661). {ECO:0000269|PubMed:27104746,
CC ECO:0000269|PubMed:27923661}.
CC -!- SIMILARITY: Belongs to the MPI phosphatase family. {ECO:0000305}.
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DR EMBL; FO081161; CCD69573.1; -; Genomic_DNA.
DR PIR; B88953; B88953.
DR PIR; T32663; T32663.
DR PIR; T32665; T32665.
DR RefSeq; NP_503446.1; NM_071045.3.
DR AlphaFoldDB; O44628; -.
DR SMR; O44628; -.
DR BioGRID; 43712; 2.
DR STRING; 6239.F16B4.8; -.
DR PaxDb; O44628; -.
DR EnsemblMetazoa; F16B4.8a.1; F16B4.8a.1; WBGene00000387.
DR GeneID; 178645; -.
DR KEGG; cel:CELE_F16B4.8; -.
DR UCSC; F16B4.8; c. elegans.
DR CTD; 178645; -.
DR WormBase; F16B4.8a; CE19796; WBGene00000387; cdc-25.2.
DR eggNOG; KOG3772; Eukaryota.
DR GeneTree; ENSGT00970000196560; -.
DR HOGENOM; CLU_548886_0_0_1; -.
DR InParanoid; O44628; -.
DR OMA; RYPFEYE; -.
DR OrthoDB; 1423329at2759; -.
DR PhylomeDB; O44628; -.
DR PRO; PR:O44628; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00000387; Expressed in embryo and 6 other tissues.
DR ExpressionAtlas; O44628; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004725; F:protein tyrosine phosphatase activity; IBA:GO_Central.
DR GO; GO:0010481; P:epidermal cell division; IMP:UniProtKB.
DR GO; GO:0000086; P:G2/M transition of mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0045138; P:nematode male tail tip morphogenesis; IMP:UniProtKB.
DR GO; GO:0010971; P:positive regulation of G2/M transition of mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0110032; P:positive regulation of G2/MI transition of meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0006470; P:protein dephosphorylation; IEA:InterPro.
DR CDD; cd01530; Cdc25; 1.
DR Gene3D; 3.40.250.10; -; 1.
DR InterPro; IPR000751; MPI_Phosphatase.
DR InterPro; IPR001763; Rhodanese-like_dom.
DR InterPro; IPR036873; Rhodanese-like_dom_sf.
DR Pfam; PF00581; Rhodanese; 1.
DR PRINTS; PR00716; MPIPHPHTASE.
DR SMART; SM00450; RHOD; 1.
DR SUPFAM; SSF52821; SSF52821; 1.
DR PROSITE; PS50206; RHODANESE_3; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Hydrolase; Mitosis; Protein phosphatase;
KW Reference proteome.
FT CHAIN 1..480
FT /note="M-phase inducer phosphatase cdc-25.2"
FT /id="PRO_0000198656"
FT DOMAIN 243..349
FT /note="Rhodanese"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00173"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 411..452
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..31
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 411..430
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 239
FT /note="S->F: In av40; Causes abnormal male tail morphology
FT when cultured at 25 degrees Celsius at the larval L2
FT stage."
FT /evidence="ECO:0000269|PubMed:27923661"
SQ SEQUENCE 480 AA; 55737 MW; 19B11B138D582FA5 CRC64;
MNRPSQISQD VAQPLSNQHE TAMMSSDEDS MSRDSGICEL MDENTPVCFS SMSTESTTVQ
VEECMEIDED ENLQPGPVSR RQKKVTFRRE KSSCQVRLFD ESPTSHKMFM RPQAPLSVRS
ENQQLLQQRK RKFEKSEQSS DHFHLEPMSV EAMDDDEIVM MDQNTTKWEP GFRAPNHKKG
RLTRSATAFP SLETFEEEEH EEQHHLNVKY HLKTVAKESS KGFRRITAET LRDIFFRLSE
KEFDDKYILI DCRYPYEYNR GHIKNAINHF DRVTVSKIFY DENGRKRCNK IPIFYCEFSQ
ARGPKMAYAL RQVDRELNVN HYPKCDYEEM YVLDLGYRNF FFAANEANIT NLCQPHAYCE
MHDKEHTMEL KKYNFHNKGQ SVLRTVSMSR SFKSLPTGSA FNFVASSASF TSAPSTSTEN
IDTNDDCQKS RTPAVPRIAS RRNLFSDPSH SPTNFAQFPL TCSRETPSPH KHPLTCPRFS
