MPI_CAEEL
ID MPI_CAEEL Reviewed; 416 AA.
AC P34650;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 3.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Probable mannose-6-phosphate isomerase;
DE EC=5.3.1.8;
DE AltName: Full=Phosphohexomutase;
DE AltName: Full=Phosphomannose isomerase;
DE Short=PMI;
GN ORFNames=ZK632.4;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Involved in the synthesis of the GDP-mannose and dolichol-
CC phosphate-mannose required for a number of critical mannosyl transfer
CC reactions. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-mannose 6-phosphate = D-fructose 6-phosphate;
CC Xref=Rhea:RHEA:12356, ChEBI:CHEBI:58735, ChEBI:CHEBI:61527;
CC EC=5.3.1.8;
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- PATHWAY: Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose
CC biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate:
CC step 1/2.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the mannose-6-phosphate isomerase type 1 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z22181; CAA80181.3; -; Genomic_DNA.
DR PIR; S40936; S40936.
DR RefSeq; NP_499174.3; NM_066773.4.
DR AlphaFoldDB; P34650; -.
DR SMR; P34650; -.
DR BioGRID; 41582; 5.
DR STRING; 6239.ZK632.4; -.
DR EPD; P34650; -.
DR PaxDb; P34650; -.
DR PeptideAtlas; P34650; -.
DR EnsemblMetazoa; ZK632.4.1; ZK632.4.1; WBGene00014013.
DR GeneID; 176388; -.
DR KEGG; cel:CELE_ZK632.4; -.
DR UCSC; ZK632.4; c. elegans.
DR CTD; 176388; -.
DR WormBase; ZK632.4; CE36417; WBGene00014013; -.
DR eggNOG; KOG2757; Eukaryota.
DR GeneTree; ENSGT00390000016075; -.
DR HOGENOM; CLU_026967_2_1_1; -.
DR InParanoid; P34650; -.
DR OMA; TEFRVLC; -.
DR OrthoDB; 1043197at2759; -.
DR PhylomeDB; P34650; -.
DR Reactome; R-CEL-446205; Synthesis of GDP-mannose.
DR UniPathway; UPA00126; UER00423.
DR PRO; PR:P34650; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00014013; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004476; F:mannose-6-phosphate isomerase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0009298; P:GDP-mannose biosynthetic process; IBA:GO_Central.
DR Gene3D; 2.60.120.10; -; 2.
DR InterPro; IPR001250; Man6P_Isoase-1.
DR InterPro; IPR016305; Mannose-6-P_Isomerase.
DR InterPro; IPR018050; Pmannose_isomerase-type1_CS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR PANTHER; PTHR10309; PTHR10309; 1.
DR Pfam; PF01238; PMI_typeI; 1.
DR PIRSF; PIRSF001480; Mannose-6-phosphate_isomerase; 1.
DR PRINTS; PR00714; MAN6PISMRASE.
DR SUPFAM; SSF51182; SSF51182; 1.
DR TIGRFAMs; TIGR00218; manA; 1.
DR PROSITE; PS00965; PMI_I_1; 1.
DR PROSITE; PS00966; PMI_I_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Isomerase; Metal-binding; Reference proteome; Zinc.
FT CHAIN 1..416
FT /note="Probable mannose-6-phosphate isomerase"
FT /id="PRO_0000194233"
FT ACT_SITE 278
FT /evidence="ECO:0000250"
FT BINDING 99
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 101
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 126
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 259
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
SQ SEQUENCE 416 AA; 46384 MW; 1C7DEDEE85DB8459 CRC64;
MLLKLKCTVN NYAWGPKGNS SMAGSLALDG GHIPNLDKDK PYAEFWVGTH ANGPAHVIEK
DIALKQLLAT SPELQGKHEK GNLSFLFKVL SVLGPLSIQI HPTKEQGKLL HATDPKNYPD
DNHKPEIAIA LTEFELLSGF RQHSQISEYL KLYSEIQELL TEEEKSQIDS LGSYGESSAQ
VLKKIFSRIW RTPKEKLQIV VDKLARRIQG HENKTALDEI IVYLFTLYPG DVGVFAPIFL
NYFKLQPGEA TFLEPNMPHA YLKGDCVECM ADSDNTIRAG LTPKYIDVES LVEMLNYDET
LLPKYIPNEL DDGSLLFTPR GIDEFWVQEV KGPAGSIYQL PYSESCSVLT VLYGTATVTL
GDASQVLNRG EVVFIGATHD AERPKINISD DFLAFRAFTP SPRALESLSN KRLIID
