MPK2_ORYSJ
ID MPK2_ORYSJ Reviewed; 394 AA.
AC Q5J4W4; Q0J7X3; Q6ZD93;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Mitogen-activated protein kinase 2;
DE Short=MAP kinase 2;
DE EC=2.7.11.24;
GN Name=MPK2; OrderedLocusNames=Os08g0157000, LOC_Os08g06060;
GN ORFNames=P0438H08.24;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Wang Y.D., Chen X., Ye Z.;
RT "A novel protein kinase OsMAPK-like involved in the developmental
RT regulation for rice plants.";
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP INDUCTION, AND NOMENCLATURE.
RX PubMed=16673940; DOI=10.1094/mpmi-19-0530;
RA Reyna N.S., Yang Y.;
RT "Molecular analysis of the rice MAP kinase gene family in relation to
RT Magnaporthe grisea infection.";
RL Mol. Plant Microbe Interact. 19:530-540(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.24;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.24;
CC -!- INDUCTION: By infection with rice blast fungus (M.grisea).
CC {ECO:0000269|PubMed:16673940}.
CC -!- PTM: The phosphorylation on Tyr-221 activates the enzyme (By
CC similarity). A conserved Thr, which must also be phosphorylated to
CC activate the enzyme in closely related sequences, is replaced by Met-
CC 219 in this sequence. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr
CC protein kinase family. MAP kinase subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC99508.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AY506572; AAS79349.1; -; mRNA.
DR EMBL; AP004460; BAC99508.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP008214; BAF22942.2; -; Genomic_DNA.
DR EMBL; AP014964; BAT03900.1; -; Genomic_DNA.
DR RefSeq; XP_015650444.1; XM_015794958.1.
DR AlphaFoldDB; Q5J4W4; -.
DR SMR; Q5J4W4; -.
DR STRING; 4530.OS08T0157000-00; -.
DR PaxDb; Q5J4W4; -.
DR PRIDE; Q5J4W4; -.
DR EnsemblPlants; Os08t0157000-00; Os08t0157000-00; Os08g0157000.
DR GeneID; 4344698; -.
DR Gramene; Os08t0157000-00; Os08t0157000-00; Os08g0157000.
DR KEGG; osa:4344698; -.
DR eggNOG; KOG0660; Eukaryota.
DR HOGENOM; CLU_000288_181_1_1; -.
DR InParanoid; Q5J4W4; -.
DR OMA; PYVAAYH; -.
DR OrthoDB; 741207at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR Genevisible; Q5J4W4; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004707; F:MAP kinase activity; IBA:GO_Central.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0004713; F:protein tyrosine kinase activity; IEA:InterPro.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR008266; Tyr_kinase_AS.
DR InterPro; IPR020635; Tyr_kinase_cat_dom.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00219; TyrKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Kinase; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..394
FT /note="Mitogen-activated protein kinase 2"
FT /id="PRO_0000239745"
FT DOMAIN 61..347
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 187
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 67..75
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 90
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 221
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 394 AA; 44585 MW; AC224C9134227481 CRC64;
MRMEGGGGGG HGHHGGGGGG HGHHGGIGGG EAQIKGTLTH GGRYVQYNVY GNLFEVSSKY
VPPIRPVGRG ACGIICAVVN AQTRQEVAIK KIGNAFDNQI DAKRTLREIK LLRHMDHDNV
ISIKDIIRPP RRENFNDVYI VYELMDTDLH HLLRSNQPLT DDHCQYFLYQ VLRGLKYVHS
ANVLHRDLRP SNLLLNAKCD LKIGDFGLAR TTNETDFMME YVVTRWYRAP ELLLNCSEYT
AAIDIWSVGC ILGEIVTREP LFPGKDYVHQ LRLITELIGS PDDSSLGFLR SDNARRYVRS
LPQYPKQQFR ARFPTMSSGA MDLLERMLVF DPSKRITVDE ALCHPYLASL HEIYDEPVCP
APFSFDFEQP SLTEEDIKEI IWREALKFNP EPIH
