ID   ARNE_KLEP7              Reviewed;         112 AA.
AC   A6TF95;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
DE            Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
DE   AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
GN   Name=arnE {ECO:0000255|HAMAP-Rule:MF_01869};
GN   OrderedLocusNames=KPN78578_38050; ORFNames=KPN_03842;
OS   Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=272620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700721 / MGH 78578;
RG   The Klebsiella pneumonia Genome Sequencing Project;
RA   McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P.,
RA   Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC       (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC       periplasmic side of the inner membrane. {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01869}.
CC   -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01869}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
CC   -!- SIMILARITY: Belongs to the ArnE family. {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
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DR   EMBL; CP000647; ABR79229.1; -; Genomic_DNA.
DR   RefSeq; WP_002920864.1; NC_009648.1.
DR   AlphaFoldDB; A6TF95; -.
DR   SMR; A6TF95; -.
DR   STRING; 272620.KPN_03842; -.
DR   DNASU; 5339826; -.
DR   EnsemblBacteria; ABR79229; ABR79229; KPN_03842.
DR   KEGG; kpn:KPN_03842; -.
DR   HOGENOM; CLU_131462_5_1_6; -.
DR   OMA; TCAGQLC; -.
DR   UniPathway; UPA00030; -.
DR   PHI-base; PHI:7954; -.
DR   Proteomes; UP000000265; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   HAMAP; MF_01869; Flippase_ArnE; 1.
DR   InterPro; IPR000620; EamA_dom.
DR   InterPro; IPR022883; Flippase_ArnE.
DR   InterPro; IPR000390; Small_drug/metabolite_transptr.
DR   PANTHER; PTHR30561; PTHR30561; 1.
DR   Pfam; PF00892; EamA; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW   Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..112
FT                   /note="Probable 4-amino-4-deoxy-L-arabinose-
FT                   phosphoundecaprenol flippase subunit ArnE"
FT                   /id="PRO_0000382978"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT   DOMAIN          35..110
FT                   /note="EamA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
SQ   SEQUENCE   112 AA;  12400 MW;  69DE0A2512D25FEB CRC64;
     MSVWICLVCA SLLSCAGQLC QKQATRPSRR GRRSRHILFW LGMALLCLGC GMLLWLSVLQ
     SIPVSIAYPM LSLNFVWVTL AGWGIWHEPV ARRHWLGVGL IVVGIVILGT SV