ID   ARNE_PSE14              Reviewed;         114 AA.
AC   Q48HY8;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
DE            Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
DE   AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
GN   Name=arnE {ECO:0000255|HAMAP-Rule:MF_01869}; OrderedLocusNames=PSPPH_2807;
OS   Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)
OS   (Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=264730;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1448A / Race 6;
RX   PubMed=16159782; DOI=10.1128/jb.187.18.6488-6498.2005;
RA   Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R.,
RA   Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M.,
RA   Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J.,
RA   Creasy T., Davidsen T.M., Haft D.H., Zafar N., Zhou L., Halpin R.,
RA   Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M.,
RA   Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A.,
RA   Buell R.;
RT   "Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola
RT   1448A reveals divergence among pathovars in genes involved in virulence and
RT   transposition.";
RL   J. Bacteriol. 187:6488-6498(2005).
CC   -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC       (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC       periplasmic side of the inner membrane. {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01869}.
CC   -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01869}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
CC   -!- SIMILARITY: Belongs to the ArnE family. {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
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DR   EMBL; CP000058; AAZ33162.1; -; Genomic_DNA.
DR   RefSeq; WP_004662222.1; NC_005773.3.
DR   AlphaFoldDB; Q48HY8; -.
DR   STRING; 264730.PSPPH_2807; -.
DR   PRIDE; Q48HY8; -.
DR   EnsemblBacteria; AAZ33162; AAZ33162; PSPPH_2807.
DR   KEGG; psp:PSPPH_2807; -.
DR   eggNOG; COG2076; Bacteria.
DR   HOGENOM; CLU_131462_5_1_6; -.
DR   OMA; TCAGQLC; -.
DR   UniPathway; UPA00030; -.
DR   Proteomes; UP000000551; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   HAMAP; MF_01869; Flippase_ArnE; 1.
DR   InterPro; IPR000620; EamA_dom.
DR   InterPro; IPR022883; Flippase_ArnE.
DR   InterPro; IPR000390; Small_drug/metabolite_transptr.
DR   PANTHER; PTHR30561; PTHR30561; 1.
DR   Pfam; PF00892; EamA; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW   Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..114
FT                   /note="Probable 4-amino-4-deoxy-L-arabinose-
FT                   phosphoundecaprenol flippase subunit ArnE"
FT                   /id="PRO_0000382988"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
SQ   SEQUENCE   114 AA;  12758 MW;  A4451797B24BCC00 CRC64;
     MTWLMLLSAC LLTCLGQIAQ KYAVEGWRET FPGVLAALRS MWLWLAIACL GLGLLVWLLV
     LQRMDVGIAY PMLGLNFVLI TLVGRYVFKE PVDPQHWLGI ALILVGVFQL GRQA