MPPA_ECOLI
ID MPPA_ECOLI Reviewed; 537 AA.
AC P77348;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Periplasmic murein peptide-binding protein;
DE Flags: Precursor;
GN Name=mppA; Synonyms=ynaH; OrderedLocusNames=b1329, JW1322;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 23-32.
RC STRAIN=K12 / AT980;
RX PubMed=9495761; DOI=10.1128/jb.180.5.1215-1223.1998;
RA Park J.T., Raychaudhuri D., Li H., Normark S., Mengin-Lecreulx D.;
RT "MppA, a periplasmic binding protein essential for import of the bacterial
RT cell wall peptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate.";
RL J. Bacteriol. 180:1215-1223(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Essential for the uptake of the murein peptide L-alanyl-
CC gamma-D-glutamyl-meso-diaminopimelate. Also transports some alpha-
CC linked peptides such as Pro-Phe-Lys with low affinity. The transport is
CC effected by the oligopeptide permease system.
CC -!- SUBCELLULAR LOCATION: Periplasm.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 5 family.
CC {ECO:0000305}.
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DR EMBL; U88242; AAC38216.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74411.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA14922.2; -; Genomic_DNA.
DR PIR; D64882; D64882.
DR RefSeq; NP_415845.2; NC_000913.3.
DR RefSeq; WP_000683020.1; NZ_STEB01000005.1.
DR PDB; 3O9P; X-ray; 2.07 A; A=23-537.
DR PDB; 4TOZ; X-ray; 1.50 A; A/B=23-537.
DR PDBsum; 3O9P; -.
DR PDBsum; 4TOZ; -.
DR AlphaFoldDB; P77348; -.
DR SMR; P77348; -.
DR BioGRID; 4260155; 288.
DR ComplexPortal; CPX-4343; Murein tripeptide ABC transporter complex.
DR ComplexPortal; CPX-4346; Heme/dipeptide ABC transporter complex, mppA variant.
DR STRING; 511145.b1329; -.
DR TCDB; 3.A.1.5.1; the atp-binding cassette (abc) superfamily.
DR TCDB; 3.A.1.5.41; the atp-binding cassette (abc) superfamily.
DR SWISS-2DPAGE; P77348; -.
DR jPOST; P77348; -.
DR PaxDb; P77348; -.
DR PRIDE; P77348; -.
DR EnsemblBacteria; AAC74411; AAC74411; b1329.
DR EnsemblBacteria; BAA14922; BAA14922; BAA14922.
DR GeneID; 945951; -.
DR KEGG; ecj:JW1322; -.
DR KEGG; eco:b1329; -.
DR PATRIC; fig|1411691.4.peg.948; -.
DR EchoBASE; EB3158; -.
DR eggNOG; COG4166; Bacteria.
DR HOGENOM; CLU_017028_0_3_6; -.
DR InParanoid; P77348; -.
DR OMA; SSWGDPQ; -.
DR PhylomeDB; P77348; -.
DR BioCyc; EcoCyc:G6665-MON; -.
DR BioCyc; MetaCyc:G6665-MON; -.
DR EvolutionaryTrace; P77348; -.
DR PRO; PR:P77348; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IDA:EcoCyc.
DR GO; GO:0042597; C:periplasmic space; IDA:EcoliWiki.
DR GO; GO:1900750; F:oligopeptide binding; IPI:EcoCyc.
DR GO; GO:1904680; F:peptide transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042938; P:dipeptide transport; IC:ComplexPortal.
DR GO; GO:0035351; P:heme transmembrane transport; IC:ComplexPortal.
DR GO; GO:0015833; P:peptide transport; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0140207; P:tripeptide import across plasma membrane; IC:ComplexPortal.
DR GO; GO:0042939; P:tripeptide transport; IMP:EcoliWiki.
DR InterPro; IPR030678; Peptide/Ni-bd.
DR InterPro; IPR039424; SBP_5.
DR InterPro; IPR023765; SBP_5_CS.
DR InterPro; IPR000914; SBP_5_dom.
DR PANTHER; PTHR30290; PTHR30290; 1.
DR Pfam; PF00496; SBP_bac_5; 1.
DR PIRSF; PIRSF002741; MppA; 1.
DR PROSITE; PS01040; SBP_BACTERIAL_5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Peptide transport; Periplasm;
KW Protein transport; Reference proteome; Signal; Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000269|PubMed:9495761"
FT CHAIN 23..537
FT /note="Periplasmic murein peptide-binding protein"
FT /id="PRO_0000031792"
FT STRAND 36..43
FT /evidence="ECO:0007829|PDB:4TOZ"
FT TURN 50..52
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 56..65
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 69..72
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 78..87
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 89..98
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 112..123
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 125..127
FT /evidence="ECO:0007829|PDB:4TOZ"
FT TURN 130..132
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 133..137
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 143..147
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 153..155
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 156..162
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 165..172
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 177..180
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 184..186
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 191..194
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 198..201
FT /evidence="ECO:0007829|PDB:4TOZ"
FT TURN 203..205
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 210..218
FT /evidence="ECO:0007829|PDB:4TOZ"
FT TURN 219..221
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 222..227
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 234..236
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 240..246
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 252..258
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 270..279
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 281..283
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 288..297
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 299..302
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 303..305
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 307..315
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 319..324
FT /evidence="ECO:0007829|PDB:4TOZ"
FT TURN 325..327
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 335..338
FT /evidence="ECO:0007829|PDB:4TOZ"
FT TURN 351..354
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 357..370
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 374..377
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 381..387
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 389..406
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 410..415
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 417..426
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 430..437
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 439..442
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 444..447
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 448..450
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 464..473
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 479..496
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 498..506
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 509..511
FT /evidence="ECO:0007829|PDB:4TOZ"
FT HELIX 529..531
FT /evidence="ECO:0007829|PDB:4TOZ"
FT STRAND 533..535
FT /evidence="ECO:0007829|PDB:4TOZ"
SQ SEQUENCE 537 AA; 59900 MW; C6A17656836DC3AC CRC64;
MKHSVSVTCC ALLVSSISLS YAAEVPSGTV LAEKQELVRH IKDEPASLDP AKAVGLPEIQ
VIRDLFEGLV NQNEKGEIVP GVATQWKSND NRIWTFTLRD NAKWADGTPV TAQDFVYSWQ
RLVDPKTLSP FAWFAALAGI NNAQAIIDGK ATPDQLGVTA VDAHTLKIQL DKPLPWFVNL
TANFAFFPVQ KANVESGKEW TKPGNLIGNG AYVLKERVVN EKLVVVPNTH YWDNAKTVLQ
KVTFLPINQE SAATKRYLAG DIDITESFPK NMYQKLLKDI PGQVYTPPQL GTYYYAFNTQ
KGPTADQRVR LALSMTIDRR LMTEKVLGTG EKPAWHFTPD VTAGFTPEPS PFEQMSQEEL
NAQAKTLLSA AGYGPQKPLK LTLLYNTSEN HQKIAIAVAS MWKKNLGVDV KLQNQEWKTY
IDSRNTGNFD VIRASWVGDY NEPSTFLTLL TSTHSGNISR FNNPAYDKVL AQASTENTVK
ARNADYNAAE KILMEQAPIA PIYQYTNGRL IKPWLKGYPI NNPEDVAYSR TMYIVKH
