ARNE_PSEAE
ID ARNE_PSEAE Reviewed; 115 AA.
AC Q9HY60;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
DE Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
DE AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
GN Name=arnE {ECO:0000255|HAMAP-Rule:MF_01869}; OrderedLocusNames=PA3557;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC periplasmic side of the inner membrane. {ECO:0000255|HAMAP-
CC Rule:MF_01869}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01869}.
CC -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000255|HAMAP-
CC Rule:MF_01869}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01869}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01869}.
CC -!- SIMILARITY: Belongs to the ArnE family. {ECO:0000255|HAMAP-
CC Rule:MF_01869}.
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DR EMBL; AE004091; AAG06945.1; -; Genomic_DNA.
DR PIR; H83201; H83201.
DR RefSeq; NP_252247.1; NC_002516.2.
DR RefSeq; WP_003112876.1; NZ_QZGE01000001.1.
DR AlphaFoldDB; Q9HY60; -.
DR STRING; 287.DR97_4385; -.
DR PaxDb; Q9HY60; -.
DR DNASU; 879132; -.
DR EnsemblBacteria; AAG06945; AAG06945; PA3557.
DR GeneID; 879132; -.
DR KEGG; pae:PA3557; -.
DR PATRIC; fig|208964.12.peg.3722; -.
DR PseudoCAP; PA3557; -.
DR HOGENOM; CLU_131462_5_1_6; -.
DR InParanoid; Q9HY60; -.
DR OMA; TCAGQLC; -.
DR BioCyc; PAER208964:G1FZ6-3625-MON; -.
DR UniPathway; UPA00030; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR HAMAP; MF_01869; Flippase_ArnE; 1.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR022883; Flippase_ArnE.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR PANTHER; PTHR30561; PTHR30561; 1.
DR Pfam; PF00892; EamA; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..115
FT /note="Probable 4-amino-4-deoxy-L-arabinose-
FT phosphoundecaprenol flippase subunit ArnE"
FT /id="PRO_0000382981"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT TRANSMEM 93..112
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT DOMAIN 46..113
FT /note="EamA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
SQ SEQUENCE 115 AA; 12496 MW; F951FFF569323582 CRC64;
MSAALLLATL LMTGLGQVAQ KLTVEHWRLV AADGWTARLR SPWPWLALLA LGLGLLCWLL
LLQRVEVGSA YPMLALNFVL VTLAARFVFD EPVDRRHLAG LLLIVAGVAL LGRSA
