MPPA_RAT
ID MPPA_RAT Reviewed; 524 AA.
AC P20069;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 25-MAY-2022, entry version 156.
DE RecName: Full=Mitochondrial-processing peptidase subunit alpha;
DE AltName: Full=Alpha-MPP;
DE AltName: Full=Inactive zinc metalloprotease alpha {ECO:0000305};
DE AltName: Full=P-55;
DE Flags: Precursor;
GN Name=Pmpca; Synonyms=Mppa;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 33-44; 113-121; 188-202;
RP 223-231; 235-242; 362-369 AND 486-511, FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RX PubMed=2236012; DOI=10.1073/pnas.87.20.7978;
RA Kleiber J., Kalousek F., Swaroop M., Rosenberg L.E.;
RT "The general mitochondrial matrix processing protease from rat liver:
RT structural characterization of the catalytic subunit.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:7978-7982(1990).
CC -!- FUNCTION: Substrate recognition and binding subunit of the essential
CC mitochondrial processing protease (MPP), which cleaves the
CC mitochondrial sequence off newly imported precursors proteins.
CC {ECO:0000250|UniProtKB:Q10713}.
CC -!- SUBUNIT: Heterodimer of PMPCA (alpha) and PMPCB (beta) subunits,
CC forming the mitochondrial processing protease (MPP) in which PMPCA is
CC involved in substrate recognition and binding and PMPCB is the
CC catalytic subunit. {ECO:0000250|UniProtKB:P11914}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix
CC {ECO:0000269|PubMed:2236012}. Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q10713}.
CC -!- SIMILARITY: Belongs to the peptidase M16 family. {ECO:0000305}.
CC -!- CAUTION: Does not seem to have a protease activity as it lack the zinc-
CC binding site. {ECO:0000305}.
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DR EMBL; M57728; AAA41632.1; -; mRNA.
DR PIR; A36205; A36205.
DR AlphaFoldDB; P20069; -.
DR SMR; P20069; -.
DR IntAct; P20069; 1.
DR STRING; 10116.ENSRNOP00000037642; -.
DR MEROPS; M16.P01; -.
DR CarbonylDB; P20069; -.
DR iPTMnet; P20069; -.
DR PhosphoSitePlus; P20069; -.
DR jPOST; P20069; -.
DR PRIDE; P20069; -.
DR UCSC; RGD:727897; rat.
DR RGD; 727897; Pmpca.
DR eggNOG; KOG2067; Eukaryota.
DR InParanoid; P20069; -.
DR PhylomeDB; P20069; -.
DR Reactome; R-RNO-8949664; Processing of SMDT1.
DR PRO; PR:P20069; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005759; C:mitochondrial matrix; TAS:RGD.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0004175; F:endopeptidase activity; IDA:RGD.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR GO; GO:0016485; P:protein processing; IDA:RGD.
DR GO; GO:0006627; P:protein processing involved in protein targeting to mitochondrion; ISS:UniProtKB.
DR InterPro; IPR011249; Metalloenz_LuxS/M16.
DR InterPro; IPR011765; Pept_M16_N.
DR InterPro; IPR001431; Pept_M16_Zn_BS.
DR InterPro; IPR007863; Peptidase_M16_C.
DR InterPro; IPR037715; PMPCA.
DR PANTHER; PTHR11851:SF192; PTHR11851:SF192; 1.
DR Pfam; PF00675; Peptidase_M16; 1.
DR Pfam; PF05193; Peptidase_M16_C; 1.
DR SUPFAM; SSF63411; SSF63411; 2.
DR PROSITE; PS00143; INSULINASE; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Transit peptide.
FT TRANSIT 1..32
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:2236012"
FT CHAIN 33..524
FT /note="Mitochondrial-processing peptidase subunit alpha"
FT /id="PRO_0000026769"
FT MOD_RES 63
FT /note="N6-succinyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9DC61"
SQ SEQUENCE 524 AA; 58608 MW; 8BF08FBC9FF09DB2 CRC64;
MATAVWAAAR LLRGSAALCA RPKFGSPAHR RFSSGATYPN IPLSSPLPGV PKPIFATVDG
QEKFETKVTT LDNGLRVASQ NKFGQFCTLG ILINSGSRYE AKYLSGIAHF LEKLAFSSTA
RFDSKDEILL TLEKHGGICD CQTSRDTTMY AVSADSKGLD TVVGLLADVV LHPRLTDEEI
EMTRMAVQFE LEDLNMRPDP EPLLTEMIHE AAFRENTVGL HRFCPVENIG KIDREVLHSY
LKNYYTPDRM VLAGVGVEHE HLVECARKYL LGVQPAWGAP GAVWMLTAQW HSTRGGSSRW
RETCQMSALR PPRFQSSHIY GGARELLLLE EDFIPFAVLN MMMGGGGSFS AGGPGKGMFS
RLYLNVLNRH HWMYNATSYH HSYEDTGLLC IHASADPRQV REMVEIITKE FILMGRTVDL
VELERAKTQL MSMLMMNLES RPVIFEDVGR QVLATHSRKL PHELCTLIRN VKPEDIKRVA
SKMLRGKPAV AALGDLTDLP TYEHIQAALS SRDGRLPRTY RLFR