MPPE1_MOUSE
ID MPPE1_MOUSE Reviewed; 396 AA.
AC Q80XL7; Q3T9I6; Q3TYP9; Q3U4F5; Q8BLU5;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Metallophosphoesterase 1;
DE EC=3.1.-.-;
DE AltName: Full=Post-GPI attachment to proteins factor 5;
GN Name=Mppe1; Synonyms=Pgap5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), AND NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] OF 1-237 (ISOFORM 1).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Aorta, Inner ear, Spleen, and Vein;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor, and Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Metallophosphoesterase required for transport of GPI-anchor
CC proteins from the endoplasmic reticulum to the Golgi. Acts in lipid
CC remodeling steps of GPI-anchor maturation by mediating the removal of a
CC side-chain ethanolamine-phosphate (EtNP) from the second Man (Man2) of
CC the GPI intermediate, an essential step for efficient transport of GPI-
CC anchor proteins (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 2 manganese ions per subunit. {ECO:0000250};
CC -!- SUBUNIT: Interacts with GPI-anchor proteins. Interacts with TMED10 (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Endoplasmic
CC reticulum-Golgi intermediate compartment membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}. Note=Also localizes to endoplasmic
CC reticulum exit site. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q80XL7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80XL7-2; Sequence=VSP_030686;
CC Name=3;
CC IsoId=Q80XL7-3; Sequence=VSP_030684, VSP_030685;
CC -!- SIMILARITY: Belongs to the metallophosphoesterase superfamily. MPPE1
CC family. {ECO:0000305}.
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DR EMBL; AK041262; BAC30883.1; -; mRNA.
DR EMBL; AK154265; BAE32476.1; -; mRNA.
DR EMBL; AK158446; BAE34513.1; -; mRNA.
DR EMBL; AK172496; BAE43034.1; -; mRNA.
DR EMBL; BC045146; AAH45146.1; -; mRNA.
DR EMBL; BC086456; AAH86456.1; -; mRNA.
DR CCDS; CCDS29319.1; -. [Q80XL7-2]
DR RefSeq; NP_766218.1; NM_172630.2. [Q80XL7-2]
DR RefSeq; XP_006525953.1; XM_006525890.2.
DR AlphaFoldDB; Q80XL7; -.
DR BioGRID; 230417; 2.
DR STRING; 10090.ENSMUSP00000072808; -.
DR iPTMnet; Q80XL7; -.
DR PhosphoSitePlus; Q80XL7; -.
DR EPD; Q80XL7; -.
DR MaxQB; Q80XL7; -.
DR PaxDb; Q80XL7; -.
DR PRIDE; Q80XL7; -.
DR ProteomicsDB; 295588; -. [Q80XL7-1]
DR ProteomicsDB; 295589; -. [Q80XL7-2]
DR ProteomicsDB; 295590; -. [Q80XL7-3]
DR Antibodypedia; 1617; 135 antibodies from 22 providers.
DR Ensembl; ENSMUST00000073054; ENSMUSP00000072808; ENSMUSG00000062526. [Q80XL7-2]
DR GeneID; 225651; -.
DR KEGG; mmu:225651; -.
DR UCSC; uc008flv.1; mouse. [Q80XL7-2]
DR UCSC; uc008flw.1; mouse. [Q80XL7-1]
DR UCSC; uc008flx.1; mouse. [Q80XL7-3]
DR CTD; 65258; -.
DR MGI; MGI:2661311; Mppe1.
DR VEuPathDB; HostDB:ENSMUSG00000062526; -.
DR eggNOG; KOG3662; Eukaryota.
DR GeneTree; ENSGT00390000013236; -.
DR HOGENOM; CLU_047168_2_0_1; -.
DR InParanoid; Q80XL7; -.
DR OMA; SRTLHCM; -.
DR OrthoDB; 1094620at2759; -.
DR PhylomeDB; Q80XL7; -.
DR TreeFam; TF314437; -.
DR BioGRID-ORCS; 225651; 2 hits in 70 CRISPR screens.
DR ChiTaRS; Mppe1; mouse.
DR PRO; PR:Q80XL7; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q80XL7; protein.
DR Bgee; ENSMUSG00000062526; Expressed in granulocyte and 215 other tissues.
DR ExpressionAtlas; Q80XL7; baseline and differential.
DR GO; GO:0005801; C:cis-Golgi network; ISS:UniProtKB.
DR GO; GO:0070971; C:endoplasmic reticulum exit site; ISS:UniProtKB.
DR GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; ISS:UniProtKB.
DR GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0034235; F:GPI anchor binding; ISS:UniProtKB.
DR GO; GO:0062050; F:GPI-mannose ethanolamine phosphate phosphodiesterase activity; ISS:UniProtKB.
DR GO; GO:0030145; F:manganese ion binding; ISS:UniProtKB.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; ISS:UniProtKB.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; ISS:UniProtKB.
DR CDD; cd08165; MPP_MPPE1; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR InterPro; IPR039541; MPP_MPPE1.
DR InterPro; IPR033308; PGAP5/Cdc1/Ted1.
DR PANTHER; PTHR13315; PTHR13315; 1.
DR Pfam; PF00149; Metallophos; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ER-Golgi transport; Golgi apparatus;
KW GPI-anchor biosynthesis; Hydrolase; Manganese; Membrane; Metal-binding;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..396
FT /note="Metallophosphoesterase 1"
FT /id="PRO_0000315729"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 75
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 117
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 117
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 155
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 249
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 303
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 305
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT VAR_SEQ 188
FT /note="N -> K (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030684"
FT VAR_SEQ 189..396
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030685"
FT VAR_SEQ 224
FT /note="E -> EQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030686"
FT CONFLICT 384
FT /note="L -> P (in Ref. 1; BAE32476)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 396 AA; 46012 MW; 8C246D289E05350B CRC64;
MALVRWGLKR QNFHPLRRRR RALLLKLTVV IISVLLFCEY FIYYLVLFRC HWPEVKTLAH
GGRQEPVLKA MFLADTHLLG EIRGHWLDKL RREWQMERAF QTALWLLQPE VVFILGDIFD
EGKWSSDQAW ADDVQRFQRM FRHDSHVQLK VVIGNHDVGF HYQMSKYRIK RFEKVFGSER
LLSLKGVNFV MVNSVAMEGD GCIICSEEEA ELREISRKLN CSQEVPGSSQ CDREPEPRLP
LSAPVLLQHY PLYRASDANC SGEDAAPPEE RNVPFEEKYD VLSREASQKL LWWLRPRLVL
SGHTHSACEV LHPGGAPEVS VPSFSWRNRN NPSFIMGSLT SRDYALSKCY LPFEDTVLTM
YGAAAGFLMI LILVHFEHLP SPFLCGWKLC RLHMRR
