MPRA_MYCBO
ID MPRA_MYCBO Reviewed; 230 AA.
AC Q7U0X4; A0A1R3XX06; Q84BX0; X2BGG7;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Response regulator MprA;
DE AltName: Full=Mycobacterial persistence regulator A;
GN Name=mprA; OrderedLocusNames=BQ2027_MB1007;
OS Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=233413;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BCG / Pasteur;
RX PubMed=14638785; DOI=10.1128/iai.71.12.6962-6970.2003;
RA Zahrt T.C., Wozniak C., Jones D., Trevett A.;
RT "Functional analysis of the Mycobacterium tuberculosis MprAB two-component
RT signal transduction system.";
RL Infect. Immun. 71:6962-6970(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT "The complete genome sequence of Mycobacterium bovis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA Robbe-Austerman S., Gordon S.V.;
RT "Updated reference genome sequence and annotation of Mycobacterium bovis
RT AF2122/97.";
RL Genome Announc. 5:E00157-E00157(2017).
RN [4]
RP INDUCTION IN MACROPHAGES.
RC STRAIN=BCG / Pasteur;
RX PubMed=11675502; DOI=10.1073/pnas.221272198;
RA Zahrt T.C., Deretic V.;
RT "Mycobacterium tuberculosis signal transduction system required for
RT persistent infections.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:12706-12711(2001).
RN [5]
RP FUNCTION AS A TRANSCRIPTIONAL REGULATOR, INDUCTION, AND AUTOREGULATION.
RC STRAIN=BCG / Pasteur;
RX PubMed=15601704; DOI=10.1128/jb.187.1.202-212.2005;
RA He H., Zahrt T.C.;
RT "Identification and characterization of a regulatory sequence recognized by
RT Mycobacterium tuberculosis persistence regulator MprA.";
RL J. Bacteriol. 187:202-212(2005).
CC -!- FUNCTION: Member of the two-component regulatory system MprB/MprA which
CC contributes to maintaining a balance among several systems involved in
CC stress resistance and is required for establishment and maintenance of
CC persistent infection in the host. Functions as a transcriptional
CC regulator that recognizes a 19-bp nucleotide motif comprizing two
CC loosely conserved 8-bp direct DNA-binding motif repeats separated by a
CC 3-bp spacer region (By similarity). MprB/MprA up-regulates expression
CC of mprA and pepD. {ECO:0000250, ECO:0000269|PubMed:15601704}.
CC -!- SUBUNIT: Monomer. Interaction with each conserved 8-bp repeat requires
CC tandem binding by two protein monomers (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Autoregulated. Induced by low concentrations of sodium
CC dodecyl sulfate (SDS). In strain BCG / Pasteur, induced during growth
CC in macrophages. {ECO:0000269|PubMed:11675502,
CC ECO:0000269|PubMed:15601704}.
CC -!- PTM: Phosphorylated and dephosphorylated by MprB. {ECO:0000250}.
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DR EMBL; AF490842; AAO85468.1; -; Genomic_DNA.
DR EMBL; LT708304; SIT99606.1; -; Genomic_DNA.
DR RefSeq; NP_854664.1; NC_002945.3.
DR RefSeq; WP_003903264.1; NC_002945.4.
DR AlphaFoldDB; Q7U0X4; -.
DR SMR; Q7U0X4; -.
DR EnsemblBacteria; SIT99606; SIT99606; BQ2027_MB1007.
DR PATRIC; fig|233413.5.peg.1096; -.
DR OMA; NYEFFGD; -.
DR Proteomes; UP000001419; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; DNA-binding; Phosphoprotein; Repressor;
KW Stress response; Transcription; Transcription regulation;
KW Two-component regulatory system; Virulence.
FT CHAIN 1..230
FT /note="Response regulator MprA"
FT /id="PRO_0000308419"
FT DOMAIN 4..118
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 129..227
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 48
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 230 AA; 25924 MW; C35D0734555BFC1C CRC64;
MSVRILVVDD DRAVRESLRR SLSFNGYSVE LAHDGVEALD MIASDRPDAL VLDVMMPRLD
GLEVCRQLRS TGDDLPILVL TARDSVSERV AGLDAGADDY LPKPFALEEL LARMRALLRR
TKPEDAAESM AMRFSDLTLD PVTREVNRGQ RRISLTRTEF ALLEMLIANP RRVLTRSRIL
EEVWGFDFPT SGNALEVYVG YLRRKTEADG EPRLIHTVRG VGYVLRETPP
