ID   MPRA_MYCPA              Reviewed;         228 AA.
AC   Q742C1;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Response regulator MprA;
DE   AltName: Full=Mycobacterial persistence regulator A;
GN   Name=mprA; OrderedLocusNames=MAP_0916;
OS   Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10)
OS   (Mycobacterium paratuberculosis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium avium complex (MAC).
OX   NCBI_TaxID=262316;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-968 / K-10;
RX   PubMed=16116077; DOI=10.1073/pnas.0505662102;
RA   Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N.,
RA   Kanjilal S., Kapur V.;
RT   "The complete genome sequence of Mycobacterium avium subspecies
RT   paratuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005).
CC   -!- FUNCTION: Member of the two-component regulatory system MprB/MprA which
CC       contributes to maintaining a balance among several systems involved in
CC       stress resistance and is required for establishment and maintenance of
CC       persistent infection in the host. Functions as a transcriptional
CC       regulator that recognizes a 19-bp nucleotide motif comprizing two
CC       loosely conserved 8-bp direct DNA-binding motif repeats separated by a
CC       3-bp spacer region (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated and dephosphorylated by MprB. {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE016958; AAS03233.1; -; Genomic_DNA.
DR   RefSeq; WP_003872811.1; NC_002944.2.
DR   AlphaFoldDB; Q742C1; -.
DR   SMR; Q742C1; -.
DR   STRING; 262316.MAP_0916; -.
DR   EnsemblBacteria; AAS03233; AAS03233; MAP_0916.
DR   GeneID; 66692821; -.
DR   KEGG; mpa:MAP_0916; -.
DR   eggNOG; COG0745; Bacteria.
DR   HOGENOM; CLU_000445_30_1_11; -.
DR   OMA; NYEFFGD; -.
DR   Proteomes; UP000000580; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome;
KW   Stress response; Transcription; Transcription regulation;
KW   Two-component regulatory system; Virulence.
FT   CHAIN           1..228
FT                   /note="Response regulator MprA"
FT                   /id="PRO_0000308422"
FT   DOMAIN          2..116
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        127..225
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         46
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   228 AA;  25681 MW;  57BB467F9F3EB339 CRC64;
     MRILVVDDDR AVRESLRRSL SFNGYSVELA HDGVEALDMI ASDRPDALVL DVMMPRLDGL
     EVCRQLRSTG DDLPILVLTA RDSVSERVAG LDAGADDYLP KPFALEELLA RMRALLRRTK
     PEDDAESVAM TFSDLTLDPV TREVTRGQRR ISLTRTEFAL LEMLIANPRR VLTRSRILEE
     VWGFDFPTSG NALEVYVGYL RRKTEADGEP RLIHTVRGVG YVLRETPP