ID   MPRA_MYCTA              Reviewed;         230 AA.
AC   A5U123;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Response regulator MprA;
DE   AltName: Full=Mycobacterial persistence regulator A;
GN   Name=mprA; OrderedLocusNames=MRA_0988;
OS   Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=419947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25177 / H37Ra;
RX   PubMed=18584054; DOI=10.1371/journal.pone.0002375;
RA   Zheng H., Lu L., Wang B., Pu S., Zhang X., Zhu G., Shi W., Zhang L.,
RA   Wang H., Wang S., Zhao G., Zhang Y.;
RT   "Genetic basis of virulence attenuation revealed by comparative genomic
RT   analysis of Mycobacterium tuberculosis strain H37Ra versus H37Rv.";
RL   PLoS ONE 3:E2375-E2375(2008).
CC   -!- FUNCTION: Member of the two-component regulatory system MprB/MprA which
CC       contributes to maintaining a balance among several systems involved in
CC       stress resistance and is required for establishment and maintenance of
CC       persistent infection in the host. Functions as a transcriptional
CC       regulator that recognizes a 19-bp nucleotide motif comprizing two
CC       loosely conserved 8-bp direct DNA-binding motif repeats separated by a
CC       3-bp spacer region (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated and dephosphorylated by MprB. {ECO:0000250}.
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DR   EMBL; CP000611; ABQ72723.1; -; Genomic_DNA.
DR   AlphaFoldDB; A5U123; -.
DR   SMR; A5U123; -.
DR   STRING; 419947.MRA_0988; -.
DR   EnsemblBacteria; ABQ72723; ABQ72723; MRA_0988.
DR   KEGG; mra:MRA_0988; -.
DR   eggNOG; COG0745; Bacteria.
DR   HOGENOM; CLU_000445_30_1_11; -.
DR   OMA; NYEFFGD; -.
DR   Proteomes; UP000001988; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Phosphoprotein; Stress response; Transcription;
KW   Transcription regulation; Two-component regulatory system; Virulence.
FT   CHAIN           1..230
FT                   /note="Response regulator MprA"
FT                   /id="PRO_0000308428"
FT   DOMAIN          4..118
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        129..227
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         48
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   230 AA;  25894 MW;  7A159FED949BE001 CRC64;
     MSVRILVVDD DRAVRESLRR SLSFNGYSVE LAHDGVEALD MIASDRPDAL VLDVMMPRLD
     GLEVCRQLRG TGDDLPILVL TARDSVSERV AGLDAGADDY LPKPFALEEL LARMRALLRR
     TKPEDAAESM AMRFSDLTLD PVTREVNRGQ RRISLTRTEF ALLEMLIANP RRVLTRSRIL
     EEVWGFDFPT SGNALEVYVG YLRRKTEADG EPRLIHTVRG VGYVLRETPP