MPR_BACSU
ID MPR_BACSU Reviewed; 313 AA.
AC P39790;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Extracellular metalloprotease;
DE EC=3.4.21.-;
DE Flags: Precursor;
GN Name=mpr; OrderedLocusNames=BSU02240;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=GP241;
RX PubMed=2105291; DOI=10.1128/jb.172.2.1024-1029.1990;
RA Sloma A., Rudolph C.F., Rufo G.A. Jr., Sullivan B.J., Theriault K.A.,
RA Ally D., Pero J.;
RT "Gene encoding a novel extracellular metalloprotease in Bacillus
RT subtilis.";
RL J. Bacteriol. 172:1024-1029(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.;
RT "Sequence analysis of the 70kb region between 17 and 23 degree of the
RT Bacillus subtilis chromosome.";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-68.
RX PubMed=3145906; DOI=10.1016/0378-1119(88)90207-7;
RA Smith H., de Jong A., Bron S., Venema G.;
RT "Characterization of signal-sequence-coding regions selected from the
RT Bacillus subtilis chromosome.";
RL Gene 70:351-361(1988).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
RC STRAIN=168;
RX PubMed=7496533; DOI=10.1099/13500872-141-9-2211;
RA Saxild H.H., Jacobsen J.H., Nygaard P.;
RT "Functional analysis of the Bacillus subtilis purT gene encoding formate-
RT dependent glycinamide ribonucleotide transformylase.";
RL Microbiology 141:2211-2218(1995).
CC -!- SUBUNIT: Monomer.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the peptidase S1B family. {ECO:0000305}.
CC -!- CAUTION: Called 'metalloprotease', but clearly belongs to the S1B
CC family of serine proteases. {ECO:0000305}.
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DR EMBL; L10505; AAA22604.1; -; Genomic_DNA.
DR EMBL; AB006424; BAA33121.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12018.1; -; Genomic_DNA.
DR EMBL; M22916; AAA22832.1; -; Genomic_DNA.
DR PIR; A35122; A35122.
DR RefSeq; NP_388106.1; NC_000964.3.
DR RefSeq; WP_003246370.1; NZ_JNCM01000030.1.
DR AlphaFoldDB; P39790; -.
DR SMR; P39790; -.
DR STRING; 224308.BSU02240; -.
DR MEROPS; S01.272; -.
DR PaxDb; P39790; -.
DR PRIDE; P39790; -.
DR DNASU; 938430; -.
DR EnsemblBacteria; CAB12018; CAB12018; BSU_02240.
DR GeneID; 938430; -.
DR KEGG; bsu:BSU02240; -.
DR PATRIC; fig|224308.179.peg.230; -.
DR eggNOG; COG3591; Bacteria.
DR InParanoid; P39790; -.
DR OMA; DTYGCQS; -.
DR PhylomeDB; P39790; -.
DR BioCyc; BSUB:BSU02240-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 2.40.10.10; -; 2.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR008256; Peptidase_S1B.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR InterPro; IPR028301; V8_his_AS.
DR InterPro; IPR000126; V8_ser_AS.
DR Pfam; PF00089; Trypsin; 1.
DR PRINTS; PR00839; V8PROTEASE.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS00672; V8_HIS; 1.
DR PROSITE; PS00673; V8_SER; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Hydrolase; Protease;
KW Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..34
FT /evidence="ECO:0000255"
FT PROPEP 35..93
FT /id="PRO_0000026902"
FT CHAIN 94..313
FT /note="Extracellular metalloprotease"
FT /id="PRO_0000026903"
FT REGION 35..74
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 146
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10083"
FT ACT_SITE 267
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10083"
FT DISULFID 131..147
FT /evidence="ECO:0000250"
FT CONFLICT 61..68
FT /note="QVSAPYEG -> PLESTAQA (in Ref. 4; AAA22832)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 313 AA; 33842 MW; D41788E8D652AE94 CRC64;
MKLVPRFRKQ WFAYLTVLCL ALAAAVSFGV PAKAAENPQT SVSNTGKEAD ATKNQTSKAD
QVSAPYEGTG KTSKSLYGGQ TELEKNIQTL QPSSIIGTDE RTRISSTTSF PYRATVQLSI
KYPNTSSTYG CTGFLVNPNT VVTAGHCVYS QDHGWASTIT AAPGRNGSSY PYGTYSGTMF
YSVKGWTESK DTNYDYGAIK LNGSPGNTVG WYGYRTTNSS SPVGLSSSVT GFPCDKTFGT
MWSDTKPIRS AETYKLTYTT DTYGCQSGSP VYRNYSDTGQ TAIAIHTNGG SSYNLGTRVT
NDVFNNIQYW ANQ
