ID   MPSS1_TRYBB             Reviewed;        1728 AA.
AC   A0A120KVR8;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   13-APR-2016, sequence version 1.
DT   25-MAY-2022, entry version 8.
DE   RecName: Full=Mitochondrial 3' processome subunit 1 {ECO:0000303|PubMed:26833087};
DE   Flags: Precursor;
GN   Name=MPSS1 {ECO:0000303|PubMed:26833087};
OS   Trypanosoma brucei brucei.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=5702;
RN   [1] {ECO:0000312|EMBL:AME15290.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, IDENTIFICATION IN THE MPSOME
RP   COMPLEX, INTERACTION WITH KRET1, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE,
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND DISRUPTION PHENOTYPE.
RX   PubMed=26833087; DOI=10.1016/j.molcel.2016.01.004;
RA   Suematsu T., Zhang L., Aphasizheva I., Monti S., Huang L., Wang Q.,
RA   Costello C.E., Aphasizhev R.;
RT   "Antisense Transcripts Delimit Exonucleolytic Activity of the Mitochondrial
RT   3' Processome to Generate Guide RNAs.";
RL   Mol. Cell 61:364-378(2016).
CC   -!- FUNCTION: As part of the mitochondrial 3' processome (MPsome), involved
CC       in the maturation of guided RNA (gRNA) precursors.
CC       {ECO:0000269|PubMed:26833087}.
CC   -!- SUBUNIT: Component of the mitochondrial 3' processome (MPsome) complex
CC       composed at least of terminal uridylyltransferase KRET1/TUT1, 3'-5'
CC       exonuclease DSS1, MPSS1, MPSS2 and MPSS3 (PubMed:26833087). Within the
CC       complex, interacts with KRET1 (PubMed:26833087).
CC       {ECO:0000269|PubMed:26833087}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:26833087}.
CC   -!- DEVELOPMENTAL STAGE: Expressed at the procyclic stage (at protein
CC       level). {ECO:0000269|PubMed:26833087}.
CC   -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown at the procyclic stage
CC       causes moderate growth defect and reduces production of guided RNAs
CC       (gRNA) due to a block in the processing of gRNA precursors.
CC       {ECO:0000269|PubMed:26833087}.
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DR   EMBL; KT282120; AME15290.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A120KVR8; -.
DR   GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR   GO; GO:0032991; C:protein-containing complex; IDA:UniProtKB.
DR   GO; GO:0080156; P:mitochondrial mRNA modification; IMP:UniProtKB.
PE   1: Evidence at protein level;
KW   Mitochondrion; Transit peptide.
FT   TRANSIT         1..117
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           118..1728
FT                   /note="Mitochondrial 3' processome subunit 1"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000450682"
FT   REGION          45..71
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          88..156
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          829..863
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        135..156
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        836..863
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1728 AA;  191362 MW;  AC4C87253A3B507A CRC64;
     MRRLILSQTL RVAGRHRPPV AMFLQRFYRG HGISTALPLH YSKRHRKREG RMYGKPLRPV
     SDGENGASGD GGVLTRWEAV VSSRHQCESP VQTLAKSEKP KKAENTVAGK MTGSSRFSHR
     RDHSAYPSGV QPAPLATSGL PTQSSERQQQ KQIGQQQVPL DALHRLFKVH AVMRTNYEAL
     GKCTKVLKSF STACAEALLQ LGVLQKEQPS AMERKGFIDV QLCKSRVTEA SKNGTSSPCF
     VLPPESPLYR TTIKFPTITV EHVLTTTVPA GCGAKDSGGD IMSVDMEGAA PFPMGHCESR
     PSTSAGNGVT VEYDCEGDVL SHGAAWNSII EGFGQLALNL KQNASVSDFA QLAETLAYFK
     WVKDEEQVGW GTEDCVVKKF REAMANAKDT SASGSDPNTS DVSAALAAIA ESFLRKIPRV
     DCIGSAVVHR GGERTAPSVH AIDVMLNLAL RMCLSQWHAM SHFERMNIVL FCTYPWFAED
     LSIAAGLCLT QSYLQSLLRA DADVTYQHTF HYVRRVSRLC WPCIHDTDSA PVKKLLFPRD
     PQGTAVSSHA LEAVSVEEKD NQLNQKEGLQ GEEQATTTSE SKLMWLPSFK DASCVSYGSS
     MKQRAAYELL LLWMTCVNPS GLHRSSPHGR YVSATGIAII DVDLFVTQRF RTGKSKDEWL
     LLHPVEGDVV ASVTIRTMVQ HCETPYALTR LFFCNFHSVL PHIWGGIGAR RREIIWTAWC
     RAVAQPLVNT LSGSDEGQRE DDGGMQTLQI TVDNADRTLD YLKPLISALT SDELFLRLIM
     RCADKSLEEM ACEIGSGNNT LPLSKWCAQL AGFVYSVAFA LHCRAKREGC NRDGGPSRPN
     TATDSANKKV VSGKQTDNLP KGTQEDISDL QLQFKAAVSA LLRKLSAGDT QVAEQLLHHF
     YEPHFSGDQE REVVEDIQRH LQQVCDSQSR DALHAWIECS ARASCEATVP LKEELLCKWQ
     RTVLEADAGL PVVLPTIVEC CRGLVGLVKK RGRNISGRRQ GQQRGTESTT VGEESSCKEV
     FSKEELPVVI TVLSRAMLTR TSTVSTVGLT KISSEISTLL SSCGYETADG NTSGGGRNGR
     ELCDTNTATI TSATAGERTV ELELILSGEI DQKAGELPWI TILEAVQLQL VPYTVVKNLL
     TSFKGGCDNG KERLRWQELQ RDFQRKRHHR LVGNTLVFRW YGCAVPNDEG WEDGSGLMRP
     MAEDRDNVHS ASHQGRTSTE LGAFTRRLLK TLARAEVTRL DDRKEITTQT FVVGPSDDSA
     TRHPTPGQEE DANLQRLDLL FRVHTLAAHI LMTASTKKPH MIHELYDTLL HLVERVMPTT
     PNAPGNASDL LLLWLVGSAV AVGLRFRPNF LIKWPSTPEV SSGNLKSCPS AEEKTVHILR
     DIEPYLQSEL LRPTVRLRFV INVLVALRSL ELLGARIDVE GLHMDQLLVR ASADRRLLSR
     HVNLFLIGCS ALQSTQSSIL HTALHLREAK YNLSFAEIER AFVAIALSAD TFARQHEALM
     EQNYQQQQNR TLSVTVAANN TTPALRVSPV QLRHAWSALG RRVLENAGES PTDLFVRGLQ
     CAAAVGCIDS ALYQQLLSYV VEFRWEDLHI LDWVMILRTV RQSFENRRNL EAYLKEPLQA
     FMLTMTDSGD NSTEQSVQKV NLKNHEGEQL LEGLCLFAEA LPGLFIGDRE LWGLLWQALG
     SQWSACFNAA SNIEEQQRIT AWLQEINASY AWAARAAGFR GLDEPTAL