ID   MPSS3_TRYBB             Reviewed;        1136 AA.
AC   A0A120KVW2;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   13-APR-2016, sequence version 1.
DT   25-MAY-2022, entry version 5.
DE   RecName: Full=Mitochondrial 3' processome subunit 3 {ECO:0000303|PubMed:26833087};
DE   Flags: Precursor;
GN   Name=MPSS3 {ECO:0000303|PubMed:26833087};
OS   Trypanosoma brucei brucei.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=5702;
RN   [1] {ECO:0000312|EMBL:AME15292.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, IDENTIFICATION IN THE MPSOME
RP   COMPLEX, INTERACTION WITH KRET1, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE,
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND DISRUPTION PHENOTYPE.
RX   PubMed=26833087; DOI=10.1016/j.molcel.2016.01.004;
RA   Suematsu T., Zhang L., Aphasizheva I., Monti S., Huang L., Wang Q.,
RA   Costello C.E., Aphasizhev R.;
RT   "Antisense Transcripts Delimit Exonucleolytic Activity of the Mitochondrial
RT   3' Processome to Generate Guide RNAs.";
RL   Mol. Cell 61:364-378(2016).
CC   -!- FUNCTION: As part of the mitochondrial 3' processome (MPsome), involved
CC       in the maturation of guided RNA (gRNA) precursors.
CC       {ECO:0000269|PubMed:26833087}.
CC   -!- SUBUNIT: Component of the mitochondrial 3' processome (MPsome) complex
CC       composed at least of terminal uridylyltransferase KRET1/TUT1, 3'-5'
CC       exonuclease DSS1, MPSS1, MPSS2 and MPSS3 (PubMed:26833087). Within the
CC       complex, interacts with KRET1 (PubMed:26833087).
CC       {ECO:0000269|PubMed:26833087}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:26833087}.
CC   -!- DEVELOPMENTAL STAGE: Expressed at the procyclic stage (at protein
CC       level). {ECO:0000269|PubMed:26833087}.
CC   -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown at the procyclic stage
CC       causes moderate growth defect without affecting the production of
CC       guided RNAs (gRNA). {ECO:0000269|PubMed:26833087}.
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DR   EMBL; KT282122; AME15292.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A120KVW2; -.
DR   GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR   GO; GO:0032991; C:protein-containing complex; IDA:UniProtKB.
DR   GO; GO:0080156; P:mitochondrial mRNA modification; IMP:UniProtKB.
PE   1: Evidence at protein level;
KW   Mitochondrion; Transit peptide.
FT   TRANSIT         1..97
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           98..1136
FT                   /note="Mitochondrial 3' processome subunit 3"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000450684"
SQ   SEQUENCE   1136 AA;  125672 MW;  6A1BF54301E1EE85 CRC64;
     MKKAWAQLER VLQPSSSRVN RALITQQLEA LYALPINCEA ACRWEDAQRL FLRCNGHRPY
     YAGIMDQSTD NRLAVASFEE NMLALQQRGL VCTTVGDKAP PCVVGGQTSR GLCRRSSIVC
     VNVGSCADLD LNMHEDCDET LLTLLHGVVV PYLHFRIVYG TGVSPPLVPR APGPAICGAS
     HDLSLQNELH MDSCVALLHP RGLCSSNCNN KAYRTAATLV RGAVSLATSD TLLHQRQDGC
     FTLHPDAMKL GSPRLFALQL WWNKEVGPVI QRGVEQSVKT DEVLSGAWIE VARKRVEELV
     ERGGDDLPQP LRNMTRDDVA AFAADATLCW TLSAFDCNYK RLGPAQPACR LQFSEEARLS
     MALELMQTVK ASLVKQPTGI PISELSATVC WPAASAWIGE KSLTEALTQF PAHFNVVNVE
     GKLVVMHGHL TGPSDNVTAE EVSEWLAAGH SEGLQKPCVA ASPSDLTFHR ETDLVVRAVA
     FLRKRHFGNV PVTYGELCGA LLPKNNGKND GDSDTLLNVL LRYDVLAADT AKGDSSINIQ
     CGLRDGDAIR LSVREDRALR ALEAFRTTSR SAFQLYTEAI EPFLTCCRGA MRENGSCVVP
     LTLLERWLQV ERLSLQSKEL LDILRSAEGP YRIDEELCNV VLTNHTAKGE VLTPAFSLPA
     TPPPPPPPAV SSRLTFEAQI SRVLKTQRPD RLLHFVQTIL HAVFDLILPH VSAPSGVPVR
     MLMRRIRWGS FVVTLGSLTS FVEAFDGLFF EVLSNASSHG EEKRDDVDLI VSAYKGPVSP
     WLLYARLIVR LFPADVDIPL GLIAEALSWS SRFAPMFGDL PSLLRRVGRQ CRNGQLLAKV
     EMVRPVCDQD DACLWELLAK IRREAHLHHL ERSSGETGQY VLLSETELYA YLPNDVKGER
     WNSTVKEEGA RCLATMAVHR LPHFFEWHVD NTDTTTRYVR VVLPFSTPPT GVVCFVEEYV
     CPLLRQHKQT TIAELDEQLG WSHGAFDAHP AGSQAAGGTP SATSLCGLLR RYVESMHSPK
     IILEPQETTL SPLHHHVHVM PNSAVYTSPE DMLLLLNGLR ISNEGITRVL PTHKPVSLFE
     LISQQLDLHQ PFREGLTTLE RGSRWNVMLA CESDSMDDCL QELDNCTGDI LVWCEG