ID   MQNA_THET8              Reviewed;         271 AA.
AC   Q5SK49;
DT   22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Chorismate dehydratase {ECO:0000255|HAMAP-Rule:MF_00995};
DE            EC=4.2.1.151 {ECO:0000255|HAMAP-Rule:MF_00995};
DE   AltName: Full=Menaquinone biosynthetic enzyme MqnA {ECO:0000255|HAMAP-Rule:MF_00995};
GN   Name=mqnA {ECO:0000255|HAMAP-Rule:MF_00995}; OrderedLocusNames=TTHA0803;
OS   Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC   Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=300852;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27634 / DSM 579 / HB8;
RA   Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA   Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT   "Complete genome sequence of Thermus thermophilus HB8.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, KINETIC PARAMETERS, AND PATHWAY.
RC   STRAIN=ATCC 27634 / DSM 579 / HB8;
RX   PubMed=24083939; DOI=10.1021/ja408594p;
RA   Mahanta N., Fedoseyenko D., Dairi T., Begley T.P.;
RT   "Menaquinone biosynthesis: formation of aminofutalosine requires a unique
RT   radical SAM enzyme.";
RL   J. Am. Chem. Soc. 135:15318-15321(2013).
CC   -!- FUNCTION: Catalyzes the dehydration of chorismate into 3-[(1-
CC       carboxyvinyl)oxy]benzoate, a step in the biosynthesis of menaquinone
CC       (MK, vitamin K2). {ECO:0000255|HAMAP-Rule:MF_00995,
CC       ECO:0000269|PubMed:24083939}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=chorismate = 3-[(1-carboxyvinyl)-oxy]benzoate + H2O;
CC         Xref=Rhea:RHEA:40051, ChEBI:CHEBI:15377, ChEBI:CHEBI:29748,
CC         ChEBI:CHEBI:76981; EC=4.2.1.151; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00995, ECO:0000269|PubMed:24083939};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=161.6 uM for chorismate {ECO:0000269|PubMed:24083939};
CC         Note=kcat is 1.27 sec(-1).;
CC   -!- PATHWAY: Quinol/quinone metabolism; menaquinone biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00995, ECO:0000269|PubMed:24083939}.
CC   -!- SIMILARITY: Belongs to the MqnA/MqnD family. MqnA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00995}.
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DR   EMBL; AP008226; BAD70626.1; -; Genomic_DNA.
DR   RefSeq; WP_011228211.1; NC_006461.1.
DR   RefSeq; YP_144069.1; NC_006461.1.
DR   AlphaFoldDB; Q5SK49; -.
DR   SMR; Q5SK49; -.
DR   STRING; 300852.55772185; -.
DR   EnsemblBacteria; BAD70626; BAD70626; BAD70626.
DR   GeneID; 3170019; -.
DR   KEGG; ttj:TTHA0803; -.
DR   PATRIC; fig|300852.9.peg.797; -.
DR   eggNOG; COG1427; Bacteria.
DR   HOGENOM; CLU_059898_0_0_0; -.
DR   OMA; WGLARTG; -.
DR   PhylomeDB; Q5SK49; -.
DR   BRENDA; 4.2.1.151; 2305.
DR   UniPathway; UPA00079; -.
DR   Proteomes; UP000000532; Chromosome.
DR   GO; GO:0016836; F:hydro-lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009234; P:menaquinone biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00995; MqnA; 1.
DR   InterPro; IPR003773; Menaquinone_biosynth.
DR   InterPro; IPR030868; MqnA.
DR   PANTHER; PTHR37690; PTHR37690; 1.
DR   Pfam; PF02621; VitK2_biosynth; 1.
PE   1: Evidence at protein level;
KW   Lyase; Menaquinone biosynthesis; Reference proteome.
FT   CHAIN           1..271
FT                   /note="Chorismate dehydratase"
FT                   /id="PRO_0000425123"
SQ   SEQUENCE   271 AA;  30453 MW;  9885BE9A28D4F282 CRC64;
     MRPYVLGLPR YANVAPLHHF LRLEGFRVLH AVPAELNRLL LSGEVGLSLV SSYFYLKHQD
     SLGLLPDFSV AVLGRVYSVN LFHKGALPHL ARVALTTESA TSVALLKLLL KEAGAGPRYE
     RREGGLELLS AYDGVLLIGD RAIKAYAALL PEVPETPHAL PTRFGEVEVA DLSTLWFQRT
     HLPFVFAVWA YRRENPPPKA LVQALREARR EGLGRLREVA EAEARRLGVH PLLLQHYLWN
     FRYHLEEPDR LGLKAFAEAL GLPFAPMFYP E