MQO2_STAAN
ID MQO2_STAAN Reviewed; 498 AA.
AC P99115; Q99R30;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Probable malate:quinone oxidoreductase 2 {ECO:0000255|HAMAP-Rule:MF_00212};
DE EC=1.1.5.4 {ECO:0000255|HAMAP-Rule:MF_00212};
DE AltName: Full=MQO 2 {ECO:0000255|HAMAP-Rule:MF_00212};
DE AltName: Full=Malate dehydrogenase [quinone] 2 {ECO:0000255|HAMAP-Rule:MF_00212};
GN Name=mqo2 {ECO:0000255|HAMAP-Rule:MF_00212}; OrderedLocusNames=SA2400;
OS Staphylococcus aureus (strain N315).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=158879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=N315;
RX PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA Hiramatsu K.;
RT "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL Lancet 357:1225-1240(2001).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=N315;
RX PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017;
RA Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez J.-C.,
RA Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.;
RT "Correlation of proteomic and transcriptomic profiles of Staphylococcus
RT aureus during the post-exponential phase of growth.";
RL J. Microbiol. Methods 60:247-257(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=N315;
RA Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.;
RT "Shotgun proteomic analysis of total and membrane protein extracts of S.
RT aureus strain N315.";
RL Submitted (OCT-2007) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate + a quinone = a quinol + oxaloacetate;
CC Xref=Rhea:RHEA:46012, ChEBI:CHEBI:15589, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.1.5.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00212};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00212};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle;
CC oxaloacetate from (S)-malate (quinone route): step 1/1.
CC {ECO:0000255|HAMAP-Rule:MF_00212}.
CC -!- SIMILARITY: Belongs to the MQO family. {ECO:0000255|HAMAP-
CC Rule:MF_00212}.
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DR EMBL; BA000018; BAB43705.1; -; Genomic_DNA.
DR PIR; G90067; G90067.
DR RefSeq; WP_001130051.1; NC_002745.2.
DR AlphaFoldDB; P99115; -.
DR SWISS-2DPAGE; P99115; -.
DR EnsemblBacteria; BAB43705; BAB43705; BAB43705.
DR KEGG; sau:SA2400; -.
DR HOGENOM; CLU_028151_0_0_9; -.
DR OMA; RCDNPEV; -.
DR UniPathway; UPA00223; UER01008.
DR Proteomes; UP000000751; Chromosome.
DR GO; GO:0052589; F:malate dehydrogenase (menaquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0008924; F:malate dehydrogenase (quinone) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniRule.
DR Gene3D; 3.50.50.60; -; 1.
DR HAMAP; MF_00212; MQO; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR006231; MQO.
DR Pfam; PF06039; Mqo; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR01320; mal_quin_oxido; 1.
PE 1: Evidence at protein level;
KW FAD; Flavoprotein; Oxidoreductase; Tricarboxylic acid cycle.
FT CHAIN 1..498
FT /note="Probable malate:quinone oxidoreductase 2"
FT /id="PRO_0000128742"
SQ SEQUENCE 498 AA; 55999 MW; 7D8D5ED706A4D183 CRC64;
MAKSNSKDIV LIGAGVLSTT FGSMLKEIEP DWNIHVYERL DRPAIESSNE RNNAGTGHAA
LCELNYTVLQ PDGSIDIEKA KVINEEFEIS KQFWGHLVKS GSIENPREFI NPLPHISYVR
GKNNVKFLKD RYEAMKAFPM FDNIEYTEDI EVMKKWIPLM MKGREDNPGI MAASKIDEGT
DVNFGELTRK MAKSIEAHPN ATVQFNHEVV DFEQLSNGQW EVTVKNRLTG EKFKQVTDYV
FIGAGGGAIP LLQKTGIPES KHLGGFPISG QFLACTNPQV IEQHDAKVYG KEPPGTPPMT
VPHLDTRYID GQRTLLFGPF ANVGPKFLKN GSNLDLFKSV KTYNITTLLA AAVKNLPLIK
YSFDQVIMTK EGCMNHLRTF YPEARNEDWQ LYTAGKRVQV IKDTPEHGKG FIQFGTEVVN
SQDHTVIALL GESPGASTSV SVALEVLERN FPEYKTEWAP KIKKMIPSYG ESLIEDEKLM
RKIRKQTSKD LELGYYEN