ARNE_YERPS
ID ARNE_YERPS Reviewed; 114 AA.
AC Q66A07; Q7BF85;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
DE Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
DE AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
GN Name=arnE {ECO:0000255|HAMAP-Rule:MF_01869}; Synonyms=pmrL;
GN OrderedLocusNames=YPTB2325;
OS Yersinia pseudotuberculosis serotype I (strain IP32953).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=273123;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=32777 / IP2777 / Serotype O1:b;
RX PubMed=15583148; DOI=10.1099/mic.0.27426-0;
RA Marceau M.B., Sebbane F., Ewann F., Collyn F., Lindner B., Campos M.A.,
RA Bengoechea J.-A., Simonet M.;
RT "The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is
RT upregulated by the PhoP-PhoQ two-component system but not by PmrA-PmrB, and
RT is not required for virulence.";
RL Microbiology 150:3947-3957(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IP32953;
RX PubMed=15358858; DOI=10.1073/pnas.0404012101;
RA Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O.,
RA Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L.,
RA Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C.,
RA Simonet M., Chenal-Francisque V., Souza B., Dacheux D., Elliott J.M.,
RA Derbise A., Hauser L.J., Garcia E.;
RT "Insights into the evolution of Yersinia pestis through whole-genome
RT comparison with Yersinia pseudotuberculosis.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004).
CC -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC periplasmic side of the inner membrane. {ECO:0000255|HAMAP-
CC Rule:MF_01869}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01869}.
CC -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000255|HAMAP-
CC Rule:MF_01869}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01869}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01869}.
CC -!- INDUCTION: Activated by low magnesium concentrations, via the two-
CC component regulatory system PhoP/PhoQ. {ECO:0000269|PubMed:15583148}.
CC -!- SIMILARITY: Belongs to the ArnE family. {ECO:0000255|HAMAP-
CC Rule:MF_01869}.
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DR EMBL; AF336802; AAK69645.1; -; Genomic_DNA.
DR EMBL; BX936398; CAH21563.1; -; Genomic_DNA.
DR RefSeq; WP_011192541.1; NZ_CP009712.1.
DR AlphaFoldDB; Q66A07; -.
DR EnsemblBacteria; CAH21563; CAH21563; YPTB2325.
DR GeneID; 66841241; -.
DR KEGG; ypo:BZ17_129; -.
DR KEGG; yps:YPTB2325; -.
DR PATRIC; fig|273123.14.peg.138; -.
DR OMA; TCAGQLC; -.
DR UniPathway; UPA00030; -.
DR Proteomes; UP000001011; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR HAMAP; MF_01869; Flippase_ArnE; 1.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR022883; Flippase_ArnE.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR PANTHER; PTHR30561; PTHR30561; 1.
DR Pfam; PF00892; EamA; 1.
PE 2: Evidence at transcript level;
KW Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW Membrane; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..114
FT /note="Probable 4-amino-4-deoxy-L-arabinose-
FT phosphoundecaprenol flippase subunit ArnE"
FT /id="PRO_0000383017"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT DOMAIN 43..112
FT /note="EamA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT CONFLICT 104
FT /note="I -> M (in Ref. 1; AAK69645)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 114 AA; 12942 MW; 3B0CF096806FBA04 CRC64;
MNSYLLLLMV SLLTCIGQLC QKQAAQCWEQ PQARRLNLTL RWLAIAVVSL GLGMLLWLRL
LQQLPLSVAY PMLSFNFVLV TLAAQLFYGE KATLRHWLGV AAIIFGILLM SWHL
