ID   MQO_MYCS2               Reviewed;         510 AA.
AC   A0QVL2; I7G8X3;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Probable malate:quinone oxidoreductase {ECO:0000255|HAMAP-Rule:MF_00212};
DE            EC=1.1.5.4 {ECO:0000255|HAMAP-Rule:MF_00212};
DE   AltName: Full=MQO {ECO:0000255|HAMAP-Rule:MF_00212};
DE   AltName: Full=Malate dehydrogenase [quinone] {ECO:0000255|HAMAP-Rule:MF_00212};
GN   Name=mqo {ECO:0000255|HAMAP-Rule:MF_00212};
GN   OrderedLocusNames=MSMEG_2613, MSMEI_2551;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS], AND CLEAVAGE OF INITIATOR METHIONINE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-malate + a quinone = a quinol + oxaloacetate;
CC         Xref=Rhea:RHEA:46012, ChEBI:CHEBI:15589, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.1.5.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00212};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00212};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle;
CC       oxaloacetate from (S)-malate (quinone route): step 1/1.
CC       {ECO:0000255|HAMAP-Rule:MF_00212}.
CC   -!- SIMILARITY: Belongs to the MQO family. {ECO:0000255|HAMAP-
CC       Rule:MF_00212}.
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DR   EMBL; CP000480; ABK72231.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP39019.1; -; Genomic_DNA.
DR   RefSeq; WP_011728472.1; NZ_SIJM01000029.1.
DR   RefSeq; YP_886950.1; NC_008596.1.
DR   AlphaFoldDB; A0QVL2; -.
DR   STRING; 246196.MSMEI_2551; -.
DR   PRIDE; A0QVL2; -.
DR   EnsemblBacteria; ABK72231; ABK72231; MSMEG_2613.
DR   EnsemblBacteria; AFP39019; AFP39019; MSMEI_2551.
DR   GeneID; 66734023; -.
DR   KEGG; msg:MSMEI_2551; -.
DR   KEGG; msm:MSMEG_2613; -.
DR   PATRIC; fig|246196.19.peg.2579; -.
DR   eggNOG; COG0579; Bacteria.
DR   OMA; PHLDTRW; -.
DR   OrthoDB; 1371190at2; -.
DR   UniPathway; UPA00223; UER01008.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0052589; F:malate dehydrogenase (menaquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0008924; F:malate dehydrogenase (quinone) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.50.50.60; -; 1.
DR   HAMAP; MF_00212; MQO; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR006231; MQO.
DR   Pfam; PF06039; Mqo; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01320; mal_quin_oxido; 1.
PE   1: Evidence at protein level;
KW   FAD; Flavoprotein; Oxidoreductase; Reference proteome;
KW   Tricarboxylic acid cycle.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:18955433"
FT   CHAIN           2..510
FT                   /note="Probable malate:quinone oxidoreductase"
FT                   /id="PRO_0000325501"
SQ   SEQUENCE   510 AA;  54914 MW;  5B7D1BA26DDFBAF3 CRC64;
     MSEANAGTNA KTDVVLVGAG IMSATLGTLI KLLEPNWSIT MIERLDGAAA ESSDPWNNAG
     TGHSALCELN YTPALPDGTI DISKAVNVNE QFQVSRQFWA HAVENGVLPD VRSFLNPVPH
     VSFVYGADNV QYLKARYNAL VTNPLFASME FIDDKDEFTR RLPLMAEKRD FSEPVALNWS
     QHGTDVDFGS LSRQLIGFAA GNGMTTMFGH DVRDLSKNSD GSWTVKVRNR RTGNNFKINA
     KFVFVGAGGG ALPLLQKSGI PEAKGFGGFP VGGAFLRTNK QHLTSRHNAK VYGLPPLGAP
     PMSVPHLDTR VINGRQWLLF GPFAGWSPKF LKQGKVTDLP LSVKPNNLAS MLGVGLTEVG
     LLKYLIGQLL LSEPARVETL REFAPSAVDS DWELDIAGQR VQVIRRKGAG GVLEFGTTVL
     AAADGSIAGL LGASPGASTA VPAMLDVLQR CFADRYQAWT PKLKEMVPSL GTKLSDEPKL
     FEEVWSWGTK VLKLDVQANT AEAANAPATV