MR30_CONMR
ID MR30_CONMR Reviewed; 289 AA.
AC A1BQQ5; A1BQQ6; A1BQQ7; A4ZXA1;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 53.
DE RecName: Full=Cysteine-rich venom protein Mr30;
DE Short=CRVP;
DE AltName: Full=Cysteine-rich secretory protein Mr30;
DE AltName: Full=GlaCrisp form 1/2/3;
DE AltName: Full=Mr30-1/2;
DE Flags: Precursor;
OS Conus marmoreus (Marble cone).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Conus.
OX NCBI_TaxID=42752;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 25-39; 64-69; 81-93;
RP 179-186; 239-248 AND 279-284, GAMMA-CARBOXYGLUTAMATION AT GLU-33, AND
RP FUNCTION (VARIANTS FORM 1/2/3).
RC TISSUE=Venom, and Venom duct;
RX PubMed=17042501; DOI=10.1021/bi061311a;
RA Hansson K., Thamlitz A.-M., Furie B., Furie B.C., Stenflo J.;
RT "A single gamma-carboxyglutamic acid residue in a novel cysteine-rich
RT secretory protein without propeptide.";
RL Biochemistry 45:12828-12839(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 25-39, FUNCTION, AND MASS
RP SPECTROMETRY (VARIANTS MR30-1 AND MR30-2).
RC TISSUE=Venom, and Venom duct;
RX PubMed=18235980; DOI=10.1111/j.1745-7270.2008.00385.x;
RA Qian J., Guo Z.Y., Chi C.W.;
RT "Cloning and isolation of a conus cysteine-rich protein homologous to Tex31
RT but without proteolytic activity.";
RL Acta Biochim. Biophys. Sin. 40:174-181(2008).
CC -!- FUNCTION: Has no proteolytic activity. {ECO:0000269|PubMed:17042501,
CC ECO:0000269|PubMed:18235980}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC -!- PTM: Contains 11 disulfide bonds.
CC -!- MASS SPECTROMETRY: Mass=29665; Method=Unknown; Note=in variant Mr30-2.;
CC Evidence={ECO:0000269|PubMed:18235980};
CC -!- MASS SPECTROMETRY: Mass=29666; Method=Unknown; Note=in variant Mr30-1.;
CC Evidence={ECO:0000269|PubMed:18235980};
CC -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; EF493183; ABP48101.1; -; mRNA.
DR EMBL; EF493184; ABP48102.1; -; mRNA.
DR EMBL; DQ647193; ABG36843.1; -; mRNA.
DR EMBL; DQ647194; ABG36844.1; -; mRNA.
DR EMBL; DQ647195; ABG36845.1; -; mRNA.
DR AlphaFoldDB; A1BQQ5; -.
DR SMR; A1BQQ5; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR001283; CRISP-related.
DR PANTHER; PTHR10334; PTHR10334; 1.
DR Pfam; PF00188; CAP; 1.
DR PRINTS; PR00837; V5TPXLIKE.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Direct protein sequencing;
KW Disulfide bond; Gamma-carboxyglutamic acid; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000269|PubMed:17042501,
FT ECO:0000269|PubMed:18235980"
FT CHAIN 25..289
FT /note="Cysteine-rich venom protein Mr30"
FT /id="PRO_0000326274"
FT DOMAIN 62..184
FT /note="SCP"
FT MOD_RES 33
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000269|PubMed:17042501"
FT VARIANT 19
FT /note="V -> A (in form 1, form 2 and Mr30-1)"
FT VARIANT 33
FT /note="E -> D (in form 1, form 2 and Mr30-1)"
FT VARIANT 49
FT /note="V -> M (in form 1)"
FT VARIANT 186
FT /note="K -> E (in Mr30-1)"
SQ SEQUENCE 289 AA; 32251 MW; FE3FE5B96C9D8F11 CRC64;
MLSTMQTVGA ILMLSIVFVA GTKRHACDSK YSEVTPTHTM CLTDNANAVA VTLTQEVKVQ
IVRMHNVIRA TVNDAANMMK MEWDDRLAAV AQKWAMQCIL GHDGFANHAE PDLPGYVGQN
VGWSNYHMTF PDVVDLWAAE IEDYEYGVWN DNTGHYIQQI YAEASRIGCG QSACGEDRYF
VCNYYKSTMG NTPYAQGSRC GQCPNSCWEE LCDCTSGPDA CCNGGSLNID TCECQCPRLW
SGADCQEKQC PDHDYEDMCD YPDVVNNPEY WCQFSNIRSD CPIRCGDCP
