ID   ARNF_ECO8A              Reviewed;         128 AA.
AC   B7M5U1;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-SEP-2009, sequence version 2.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE            Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE   AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
GN   Name=arnF {ECO:0000255|HAMAP-Rule:MF_00538}; OrderedLocusNames=ECIAI1_2336;
OS   Escherichia coli O8 (strain IAI1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=585034;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IAI1;
RX   PubMed=19165319; DOI=10.1371/journal.pgen.1000344;
RA   Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P.,
RA   Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H.,
RA   Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E.,
RA   Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C.,
RA   Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S.,
RA   Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J.,
RA   Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E.;
RT   "Organised genome dynamics in the Escherichia coli species results in
RT   highly diverse adaptive paths.";
RL   PLoS Genet. 5:E1000344-E1000344(2009).
CC   -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC       (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC       periplasmic side of the inner membrane. {ECO:0000255|HAMAP-
CC       Rule:MF_00538}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00538}.
CC   -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000255|HAMAP-
CC       Rule:MF_00538}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00538}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00538}.
CC   -!- SIMILARITY: Belongs to the ArnF family. {ECO:0000255|HAMAP-
CC       Rule:MF_00538}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAQ99178.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CU928160; CAQ99178.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_000523879.1; NC_011741.1.
DR   AlphaFoldDB; B7M5U1; -.
DR   KEGG; ecr:ECIAI1_2336; -.
DR   HOGENOM; CLU_1243704_0_0_6; -.
DR   UniPathway; UPA00030; -.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   HAMAP; MF_00538; Flippase_ArnF; 1.
DR   InterPro; IPR022832; Flippase_ArnF.
DR   InterPro; IPR000390; Small_drug/metabolite_transptr.
DR   PANTHER; PTHR30561; PTHR30561; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW   Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..128
FT                   /note="Probable 4-amino-4-deoxy-L-arabinose-
FT                   phosphoundecaprenol flippase subunit ArnF"
FT                   /id="PRO_0000382003"
FT   TOPO_DOM        1..2
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TOPO_DOM        24..35
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TOPO_DOM        57..76
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TOPO_DOM        98..100
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TRANSMEM        101..121
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TOPO_DOM        122..128
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
SQ   SEQUENCE   128 AA;  14115 MW;  1ACEEE17FDE18446 CRC64;
     MGLMWGLFSV IIASVAQLSL GFAASHLPPM THLWDFIAAL LAFGLDARIL LLGLLGYLLS
     VFCWYKTLHK LALSKAYALL SMSYVLVWIA SMVLPGWEGT FSLKALLGVA CIMSGLMLIF
     LPMTKQRY