ARNF_ECODH
ID ARNF_ECODH Reviewed; 128 AA.
AC B1X8X2;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
GN Name=arnF {ECO:0000255|HAMAP-Rule:MF_00538};
GN OrderedLocusNames=ECDH10B_2419;
OS Escherichia coli (strain K12 / DH10B).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=316385;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / DH10B;
RX PubMed=18245285; DOI=10.1128/jb.01695-07;
RA Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B.,
RA Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B.,
RA Posfai G., Weinstock G.M., Blattner F.R.;
RT "The complete genome sequence of Escherichia coli DH10B: insights into the
RT biology of a laboratory workhorse.";
RL J. Bacteriol. 190:2597-2606(2008).
CC -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC periplasmic side of the inner membrane. {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00538}.
CC -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_00538}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC -!- SIMILARITY: Belongs to the ArnF family. {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
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DR EMBL; CP000948; ACB03419.1; -; Genomic_DNA.
DR RefSeq; WP_000523880.1; NC_010473.1.
DR AlphaFoldDB; B1X8X2; -.
DR GeneID; 58461865; -.
DR KEGG; ecd:ECDH10B_2419; -.
DR HOGENOM; CLU_131462_1_0_6; -.
DR OMA; AQLGMRW; -.
DR BioCyc; ECOL316385:ECDH10B_RS12305-MON; -.
DR UniPathway; UPA00030; -.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR HAMAP; MF_00538; Flippase_ArnF; 1.
DR InterPro; IPR022832; Flippase_ArnF.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR PANTHER; PTHR30561; PTHR30561; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW Membrane; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..128
FT /note="Probable 4-amino-4-deoxy-L-arabinose-
FT phosphoundecaprenol flippase subunit ArnF"
FT /id="PRO_1000128661"
FT TOPO_DOM 1..2
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 24..35
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 57..76
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 98..100
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 122..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
SQ SEQUENCE 128 AA; 14085 MW; 1ACEEE17FDF6B446 CRC64;
MGLMWGLFSV IIASVAQLSL GFAASHLPPM THLWDFIAAL LAFGLDARIL LLGLLGYLLS
VFCWYKTLHK LALSKAYALL SMSYVLVWIA SMVLPGWEGT FSLKALLGVA CIMSGLMLIF
LPTTKQRY
