ARNF_ECOUT
ID ARNF_ECOUT Reviewed; 128 AA.
AC Q1R9F6;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-SEP-2009, sequence version 2.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
GN Name=arnF {ECO:0000255|HAMAP-Rule:MF_00538}; OrderedLocusNames=UTI89_C2541;
OS Escherichia coli (strain UTI89 / UPEC).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=364106;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTI89 / UPEC;
RX PubMed=16585510; DOI=10.1073/pnas.0600938103;
RA Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A.,
RA Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S.,
RA Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J.,
RA Gordon J.I.;
RT "Identification of genes subject to positive selection in uropathogenic
RT strains of Escherichia coli: a comparative genomics approach.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006).
CC -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC periplasmic side of the inner membrane. {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00538}.
CC -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_00538}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC -!- SIMILARITY: Belongs to the ArnF family. {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABE08008.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000243; ABE08008.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_000523876.1; NC_007946.1.
DR AlphaFoldDB; Q1R9F6; -.
DR EnsemblBacteria; ABE08008; ABE08008; UTI89_C2541.
DR KEGG; eci:UTI89_C2541; -.
DR HOGENOM; CLU_1243704_0_0_6; -.
DR OMA; AQLGMRW; -.
DR UniPathway; UPA00030; -.
DR Proteomes; UP000001952; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR HAMAP; MF_00538; Flippase_ArnF; 1.
DR InterPro; IPR022832; Flippase_ArnF.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR PANTHER; PTHR30561; PTHR30561; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW Membrane; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..128
FT /note="Probable 4-amino-4-deoxy-L-arabinose-
FT phosphoundecaprenol flippase subunit ArnF"
FT /id="PRO_0000381999"
FT TOPO_DOM 1..2
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 24..35
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 57..76
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 98..100
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 122..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
SQ SEQUENCE 128 AA; 14075 MW; B1AD7D80E1107B63 CRC64;
MGLMWGLFSV IIASAAQLSM GFAASHLPPM THLWDFIAAL LAFGLDARIL LLGLLGYLLS
VFCWYKTLHK LALSKAYALL SMSYVLVWIA SMVLPGWEGT FSLKALLGVA CIMSGLMLIF
LPTTKQRY
