ID   ARNF_PSEAB              Reviewed;         137 AA.
AC   Q02R29;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE            Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE   AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
GN   Name=arnF {ECO:0000255|HAMAP-Rule:MF_00538}; OrderedLocusNames=PA14_18310;
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14;
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA   Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
CC   -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC       (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC       periplasmic side of the inner membrane. {ECO:0000255|HAMAP-
CC       Rule:MF_00538}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00538}.
CC   -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000255|HAMAP-
CC       Rule:MF_00538}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00538}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00538}.
CC   -!- SIMILARITY: Belongs to the ArnF family. {ECO:0000255|HAMAP-
CC       Rule:MF_00538}.
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DR   EMBL; CP000438; ABJ12792.1; -; Genomic_DNA.
DR   RefSeq; WP_003138074.1; NZ_CP034244.1.
DR   AlphaFoldDB; Q02R29; -.
DR   EnsemblBacteria; ABJ12792; ABJ12792; PA14_18310.
DR   KEGG; pau:PA14_18310; -.
DR   HOGENOM; CLU_131462_1_0_6; -.
DR   OMA; AQLGMRW; -.
DR   BioCyc; PAER208963:G1G74-1510-MON; -.
DR   UniPathway; UPA00030; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   HAMAP; MF_00538; Flippase_ArnF; 1.
DR   InterPro; IPR022832; Flippase_ArnF.
DR   InterPro; IPR000390; Small_drug/metabolite_transptr.
DR   PANTHER; PTHR30561; PTHR30561; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW   Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..137
FT                   /note="Probable 4-amino-4-deoxy-L-arabinose-
FT                   phosphoundecaprenol flippase subunit ArnF"
FT                   /id="PRO_0000382011"
FT   TOPO_DOM        1..3
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TOPO_DOM        25..44
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TOPO_DOM        66..76
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TOPO_DOM        98..100
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TRANSMEM        101..121
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TOPO_DOM        122..137
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
SQ   SEQUENCE   137 AA;  14335 MW;  A9B9714006DF531D CRC64;
     MNALRGWLAA LGSMLLASAA QLGMRWGMSR LPLPEAWAGQ TPERAALLAV ALAVTAYAAS
     LLCWLAALRH LPLGRAYSLL SASYALVYLL AASLPAFDET FSTSKTLGVG LVVLGVLTVN
     ARRTAAAPAH HPSRKAP