ARNF_PSEPF
ID ARNF_PSEPF Reviewed; 134 AA.
AC Q3KCB7;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
GN Name=arnF {ECO:0000255|HAMAP-Rule:MF_00538}; OrderedLocusNames=Pfl01_2847;
OS Pseudomonas fluorescens (strain Pf0-1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=205922;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Pf0-1;
RX PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT "Genomic and genetic analyses of diversity and plant interactions of
RT Pseudomonas fluorescens.";
RL Genome Biol. 10:R51.1-R51.16(2009).
CC -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC periplasmic side of the inner membrane. {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00538}.
CC -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_00538}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC -!- SIMILARITY: Belongs to the ArnF family. {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
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DR EMBL; CP000094; ABA74588.1; -; Genomic_DNA.
DR RefSeq; WP_011334259.1; NC_007492.2.
DR AlphaFoldDB; Q3KCB7; -.
DR STRING; 205922.Pfl01_2847; -.
DR EnsemblBacteria; ABA74588; ABA74588; Pfl01_2847.
DR KEGG; pfo:Pfl01_2847; -.
DR eggNOG; COG2076; Bacteria.
DR HOGENOM; CLU_131462_1_0_6; -.
DR OMA; AQLGMRW; -.
DR UniPathway; UPA00030; -.
DR Proteomes; UP000002704; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR HAMAP; MF_00538; Flippase_ArnF; 1.
DR InterPro; IPR022832; Flippase_ArnF.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR PANTHER; PTHR30561; PTHR30561; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW Membrane; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..134
FT /note="Probable 4-amino-4-deoxy-L-arabinose-
FT phosphoundecaprenol flippase subunit ArnF"
FT /id="PRO_0000382013"
FT TOPO_DOM 1..5
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 27..45
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 67..77
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 99..101
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 123..134
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
SQ SEQUENCE 134 AA; 14625 MW; 24D92BC3B67E048E CRC64;
MNRRRGILFA LASVLLVSVA QLSMRWSMTR LPRPDQWLSV PSVDSVALAV VLAAIFAYAL
SMLCWLAALR DLPLGRAYSL LSISYALVYL LAASLPLFNE SFSFSKSLGV ALVMLGVITI
NTRPARAPEL RSSP
