ARNF_SALTY
ID ARNF_SALTY Reviewed; 125 AA.
AC Q8ZNF0; O52329;
DT 21-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2002, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF;
DE Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnF;
DE AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnF;
GN Name=arnF; OrderedLocusNames=STM2303;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 14028s / SGSG 2262;
RX PubMed=9570402; DOI=10.1046/j.1365-2958.1998.00757.x;
RA Gunn J.S., Lim K.B., Krueger J., Kim K., Guo L., Hackett M., Miller S.I.;
RT "PmrA-PmrB-regulated genes necessary for 4-aminoarabinose lipid A
RT modification and polymyxin resistance.";
RL Mol. Microbiol. 27:1171-1182(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 14028s / SGSG 2262;
RX PubMed=11035717; DOI=10.1128/iai.68.11.6139-6146.2000;
RA Gunn J.S., Ryan S.S., Van Velkinburgh J.C., Ernst R.K., Miller S.I.;
RT "Genetic and functional analysis of a PmrA-PmrB-regulated locus necessary
RT for lipopolysaccharide modification, antimicrobial peptide resistance, and
RT oral virulence of Salmonella enterica serovar typhimurium.";
RL Infect. Immun. 68:6139-6146(2000).
CC -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC periplasmic side of the inner membrane. {ECO:0000250}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis.
CC -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: No change observed, indicating that it could be
CC not necessary for the Ara4N addition to lipid A and polymyxin
CC resistance. {ECO:0000269|PubMed:11035717}.
CC -!- SIMILARITY: Belongs to the ArnF family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF036677; AAC04776.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21204.1; -; Genomic_DNA.
DR RefSeq; NP_461245.1; NC_003197.2.
DR RefSeq; WP_000538694.1; NC_003197.2.
DR AlphaFoldDB; Q8ZNF0; -.
DR STRING; 99287.STM2303; -.
DR PaxDb; Q8ZNF0; -.
DR EnsemblBacteria; AAL21204; AAL21204; STM2303.
DR GeneID; 1253825; -.
DR KEGG; stm:STM2303; -.
DR PATRIC; fig|99287.12.peg.2438; -.
DR HOGENOM; CLU_131462_1_0_6; -.
DR OMA; AQLGMRW; -.
DR PhylomeDB; Q8ZNF0; -.
DR BioCyc; SENT99287:STM2303-MON; -.
DR UniPathway; UPA00030; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR HAMAP; MF_00538; Flippase_ArnF; 1.
DR InterPro; IPR022832; Flippase_ArnF.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR PANTHER; PTHR30561; PTHR30561; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..125
FT /note="Probable 4-amino-4-deoxy-L-arabinose-
FT phosphoundecaprenol flippase subunit ArnF"
FT /id="PRO_0000218157"
FT TOPO_DOM 1..2
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 24..33
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..76
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..100
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 122..125
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 74
FT /note="S -> N (in Ref. 1; AAC04776)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 125 AA; 13121 MW; 54410362A1DD6AFF CRC64;
MGVMWGLISV AIASLAQLSL GFAMMRLPSI AHPLAFISGL GAFNAATLAL FAGLAGYLVS
VFCWQKTLHT LALSKAYALL SLSYVLVWVA SMLLPGLQGA FSLKAMLGVL CIMAGVMLIF
LPARS
