ID   ARNF_SERP5              Reviewed;         130 AA.
AC   A8GDS1;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE            Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE   AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
GN   Name=arnF {ECO:0000255|HAMAP-Rule:MF_00538}; OrderedLocusNames=Spro_2160;
OS   Serratia proteamaculans (strain 568).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Serratia.
OX   NCBI_TaxID=399741;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=568;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L.,
RA   Vangronsveld J., van der Lelie D., Richardson P.;
RT   "Complete sequence of chromosome of Serratia proteamaculans 568.";
RL   Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC       (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC       periplasmic side of the inner membrane. {ECO:0000255|HAMAP-
CC       Rule:MF_00538}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00538}.
CC   -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000255|HAMAP-
CC       Rule:MF_00538}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00538}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00538}.
CC   -!- SIMILARITY: Belongs to the ArnF family. {ECO:0000255|HAMAP-
CC       Rule:MF_00538}.
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DR   EMBL; CP000826; ABV41261.1; -; Genomic_DNA.
DR   RefSeq; WP_012144892.1; NC_009832.1.
DR   AlphaFoldDB; A8GDS1; -.
DR   STRING; 399741.Spro_2160; -.
DR   EnsemblBacteria; ABV41261; ABV41261; Spro_2160.
DR   KEGG; spe:Spro_2160; -.
DR   eggNOG; COG2076; Bacteria.
DR   HOGENOM; CLU_131462_1_0_6; -.
DR   OMA; AQLGMRW; -.
DR   OrthoDB; 1944417at2; -.
DR   UniPathway; UPA00030; -.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   HAMAP; MF_00538; Flippase_ArnF; 1.
DR   InterPro; IPR022832; Flippase_ArnF.
DR   InterPro; IPR000390; Small_drug/metabolite_transptr.
DR   PANTHER; PTHR30561; PTHR30561; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW   Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..130
FT                   /note="Probable 4-amino-4-deoxy-L-arabinose-
FT                   phosphoundecaprenol flippase subunit ArnF"
FT                   /id="PRO_1000061040"
FT   TOPO_DOM        1..4
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TOPO_DOM        26..47
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TRANSMEM        48..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TOPO_DOM        69..77
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TRANSMEM        78..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TOPO_DOM        99
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT   TOPO_DOM        121..130
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
SQ   SEQUENCE   130 AA;  14559 MW;  227B189D2E8B7695 CRC64;
     MRGYAWGAAS VLLVTLAQLL MKWGMAQIPL MSFADVTLNL FMQYWLPLVV VSGGIFGYAL
     SMLCWFFALH HLPLNRAYPL LSVSYALVYL AAVILPWFNE SATLLKTLGT LFILFGVWLI
     NSQAKVKTPQ