ARNF_YERPS
ID ARNF_YERPS Reviewed; 128 AA.
AC Q93PD4; Q66A08;
DT 21-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 2.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
DE AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnF {ECO:0000255|HAMAP-Rule:MF_00538};
GN Name=arnF {ECO:0000255|HAMAP-Rule:MF_00538}; OrderedLocusNames=YPTB2324;
OS Yersinia pseudotuberculosis serotype I (strain IP32953).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=273123;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=32777 / IP2777 / Serotype O1:b;
RX PubMed=15583148; DOI=10.1099/mic.0.27426-0;
RA Marceau M.B., Sebbane F., Ewann F., Collyn F., Lindner B., Campos M.A.,
RA Bengoechea J.-A., Simonet M.;
RT "The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is
RT upregulated by the PhoP-PhoQ two-component system but not by PmrA-PmrB, and
RT is not required for virulence.";
RL Microbiology 150:3947-3957(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IP32953;
RX PubMed=15358858; DOI=10.1073/pnas.0404012101;
RA Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O.,
RA Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L.,
RA Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C.,
RA Simonet M., Chenal-Francisque V., Souza B., Dacheux D., Elliott J.M.,
RA Derbise A., Hauser L.J., Garcia E.;
RT "Insights into the evolution of Yersinia pestis through whole-genome
RT comparison with Yersinia pseudotuberculosis.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004).
CC -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC periplasmic side of the inner membrane. {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00538}.
CC -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_00538}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC -!- INDUCTION: Activated by low magnesium concentrations, via the two-
CC component regulatory system PhoP/PhoQ. {ECO:0000269|PubMed:15583148}.
CC -!- SIMILARITY: Belongs to the ArnF family. {ECO:0000255|HAMAP-
CC Rule:MF_00538}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF336802; AAK69646.1; -; Genomic_DNA.
DR EMBL; BX936398; CAH21562.1; -; Genomic_DNA.
DR RefSeq; WP_002211819.1; NZ_CP009712.1.
DR AlphaFoldDB; Q93PD4; -.
DR EnsemblBacteria; CAH21562; CAH21562; YPTB2324.
DR GeneID; 66841242; -.
DR KEGG; ypo:BZ17_130; -.
DR KEGG; yps:YPTB2324; -.
DR PATRIC; fig|273123.14.peg.139; -.
DR OMA; AQLGMRW; -.
DR UniPathway; UPA00030; -.
DR Proteomes; UP000001011; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR HAMAP; MF_00538; Flippase_ArnF; 1.
DR InterPro; IPR022832; Flippase_ArnF.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR PANTHER; PTHR30561; PTHR30561; 1.
PE 2: Evidence at transcript level;
KW Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW Membrane; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..128
FT /note="Probable 4-amino-4-deoxy-L-arabinose-
FT phosphoundecaprenol flippase subunit ArnF"
FT /id="PRO_0000218161"
FT TOPO_DOM 1..10
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 32..47
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 69..77
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 99..101
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT TOPO_DOM 123..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00538"
FT CONFLICT 56
FT /note="M -> I (in Ref. 1; AAK69646)"
FT /evidence="ECO:0000305"
FT CONFLICT 59
FT /note="A -> S (in Ref. 1; AAK69646)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 128 AA; 14223 MW; 33AC97482B8E21B4 CRC64;
MKGYLWGGAS VVLVTVAQLV LKWGMMNIPL LSLADINVQF LTMYFVQLAS VMCGLMGYAL
SMLCWFFALR YLPLNRAYPL LSLSYALVYL GAVLLPWFNE PATLLKTLGA GFILLGIWLI
NIKPIKAS
