ARNT2_MOUSE
ID ARNT2_MOUSE Reviewed; 712 AA.
AC Q61324; Q7TQG2; Q8CHG9;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Aryl hydrocarbon receptor nuclear translocator 2;
DE Short=ARNT protein 2;
GN Name=Arnt2; Synonyms=Kiaa0307;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=8657146; DOI=10.1128/mcb.16.4.1706;
RA Hirose K., Morita M., Ema M., Mimura J., Hamada H., Fujii H., Saijo Y.,
RA Gotoh O., Sogawa K., Fujii-Kuriyama Y.;
RT "cDNA cloning and tissue-specific expression of a novel basic helix-loop-
RT helix/PAS factor (Arnt2) with close sequence similarity to the aryl
RT hydrocarbon receptor nuclear translocator (Arnt).";
RL Mol. Cell. Biol. 16:1706-1713(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=12465718; DOI=10.1093/dnares/9.5.179;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: I.
RT The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 9:179-188(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH TACC3.
RX PubMed=11025203; DOI=10.1016/s0925-4773(00)00415-9;
RA Sadek C.M., Jalaguier S., Feeney E.P., Aitola M., Damdimopoulos A.E.,
RA Pelto-Huikko M., Gustafsson J.-A.;
RT "Isolation and characterization of AINT: a novel ARNT interacting protein
RT expressed during murine embryonic development.";
RL Mech. Dev. 97:13-26(2000).
RN [5]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-42, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
RN [6]
RP INTERACTION WITH SIM1 AND NPAS4, AND MUTAGENESIS OF LEU-86; LEU-106;
RP LEU-141; ALA-145; ILE-238; VAL-279; ARG-340 AND ASN-422.
RX PubMed=27782878; DOI=10.7554/elife.18790;
RA Wu D., Su X., Potluri N., Kim Y., Rastinejad F.;
RT "NPAS1-ARNT and NPAS3-ARNT crystal structures implicate the bHLH-PAS family
RT as multi-ligand binding transcription factors.";
RL Elife 5:0-0(2016).
CC -!- FUNCTION: Transcription factor that plays a role in the development of
CC the hypothalamo-pituitary axis, postnatal brain growth, and visual and
CC renal function. Specifically recognizes the xenobiotic response element
CC (XRE). {ECO:0000250|UniProtKB:Q9HBZ2}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein (By similarity). Heterodimer with NPAS4 or SIM1
CC (PubMed:27782878). Heterodimer with the aryl hydrocarbon receptor (AHR)
CC or the SIM1 protein (By similarity). Interacts with TACC3
CC (PubMed:11025203). {ECO:0000250|UniProtKB:Q9HBZ2,
CC ECO:0000269|PubMed:11025203, ECO:0000269|PubMed:27782878}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9HBZ2,
CC ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q61324-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q61324-2; Sequence=VSP_022689;
CC -!- TISSUE SPECIFICITY: Restricted to adult brain and kidney.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC41409.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; D63644; BAA09799.1; -; mRNA.
DR EMBL; AB093225; BAC41409.1; ALT_INIT; mRNA.
DR EMBL; BC054546; AAH54546.1; -; mRNA.
DR CCDS; CCDS59733.1; -. [Q61324-1]
DR RefSeq; NP_031514.3; NM_007488.3. [Q61324-1]
DR RefSeq; XP_006507303.1; XM_006507240.2. [Q61324-2]
DR RefSeq; XP_006507305.1; XM_006507242.2. [Q61324-2]
DR RefSeq; XP_011239957.1; XM_011241655.2. [Q61324-2]
DR AlphaFoldDB; Q61324; -.
DR SMR; Q61324; -.
DR BioGRID; 198206; 25.
DR IntAct; Q61324; 2.
DR STRING; 10090.ENSMUSP00000082154; -.
DR iPTMnet; Q61324; -.
DR PhosphoSitePlus; Q61324; -.
DR MaxQB; Q61324; -.
DR PaxDb; Q61324; -.
DR PeptideAtlas; Q61324; -.
DR PRIDE; Q61324; -.
DR ProteomicsDB; 283273; -. [Q61324-1]
DR ProteomicsDB; 283274; -. [Q61324-2]
DR Antibodypedia; 3911; 297 antibodies from 32 providers.
DR DNASU; 11864; -.
DR Ensembl; ENSMUST00000085077; ENSMUSP00000082154; ENSMUSG00000015709. [Q61324-1]
DR Ensembl; ENSMUST00000208232; ENSMUSP00000146413; ENSMUSG00000015709. [Q61324-2]
DR Ensembl; ENSMUST00000209133; ENSMUSP00000147129; ENSMUSG00000015709. [Q61324-2]
DR GeneID; 11864; -.
DR KEGG; mmu:11864; -.
DR UCSC; uc009ief.2; mouse. [Q61324-1]
DR CTD; 9915; -.
DR MGI; MGI:107188; Arnt2.
DR VEuPathDB; HostDB:ENSMUSG00000015709; -.
DR eggNOG; KOG3561; Eukaryota.
DR GeneTree; ENSGT00940000158198; -.
DR HOGENOM; CLU_011864_1_1_1; -.
DR InParanoid; Q61324; -.
DR OMA; MRPFSAN; -.
DR OrthoDB; 331262at2759; -.
DR PhylomeDB; Q61324; -.
DR TreeFam; TF319983; -.
DR Reactome; R-MMU-211945; Phase I - Functionalization of compounds.
DR Reactome; R-MMU-211976; Endogenous sterols.
DR Reactome; R-MMU-211981; Xenobiotics.
DR Reactome; R-MMU-8937144; Aryl hydrocarbon receptor signalling.
DR BioGRID-ORCS; 11864; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Arnt2; mouse.
DR PRO; PR:Q61324; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q61324; protein.
DR Bgee; ENSMUSG00000015709; Expressed in retrosplenial region and 227 other tissues.
DR ExpressionAtlas; Q61324; baseline and differential.
DR Genevisible; Q61324; MM.
DR GO; GO:0034751; C:aryl hydrocarbon receptor complex; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR GO; GO:0017162; F:aryl hydrocarbon receptor binding; IPI:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0046982; F:protein heterodimerization activity; IDA:UniProtKB.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0007420; P:brain development; ISS:UniProtKB.
DR GO; GO:0007417; P:central nervous system development; IMP:UniProtKB.
DR GO; GO:0001701; P:in utero embryonic development; IEP:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0001666; P:response to hypoxia; ISO:MGI.
DR GO; GO:0009636; P:response to toxic substance; TAS:UniProtKB.
DR GO; GO:0009410; P:response to xenobiotic stimulus; TAS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; NAS:UniProtKB.
DR CDD; cd00130; PAS; 2.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR001067; Nuc_translocat.
DR InterPro; IPR001610; PAC.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR013767; PAS_fold.
DR Pfam; PF00010; HLH; 1.
DR Pfam; PF00989; PAS; 1.
DR PRINTS; PR00785; NCTRNSLOCATR.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00086; PAC; 1.
DR SMART; SM00091; PAS; 2.
DR SUPFAM; SSF47459; SSF47459; 1.
DR SUPFAM; SSF55785; SSF55785; 2.
DR TIGRFAMs; TIGR00229; sensory_box; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS50112; PAS; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Methylation; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..712
FT /note="Aryl hydrocarbon receptor nuclear translocator 2"
FT /id="PRO_0000127123"
FT DOMAIN 63..116
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 134..209
FT /note="PAS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 323..393
FT /note="PAS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 398..441
FT /note="PAC"
FT REGION 36..73
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 573..712
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 46..73
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 573..678
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 42
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT VAR_SEQ 1..11
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12465718"
FT /id="VSP_022689"
FT MUTAGEN 86
FT /note="L->E: Compromises SIM1:ARNT2 heterodimer stability.
FT Compromises NPAS4:ARNT2 heterodimer stability."
FT /evidence="ECO:0000269|PubMed:27782878"
FT MUTAGEN 106
FT /note="L->E: Compromises SIM1:ARNT2 heterodimer stability.
FT Compromises NPAS4:ARNT2 heterodimer stability."
FT /evidence="ECO:0000269|PubMed:27782878"
FT MUTAGEN 141
FT /note="L->E: Compromises SIM1:ARNT2 heterodimer stability.
FT Does not compromises NPAS4:ARNT2 heterodimer stability."
FT /evidence="ECO:0000269|PubMed:27782878"
FT MUTAGEN 145
FT /note="A->D: Compromises SIM1:ARNT2 heterodimer stability.
FT Does not compromises NPAS4:ARNT2 heterodimer stability."
FT /evidence="ECO:0000269|PubMed:27782878"
FT MUTAGEN 238
FT /note="I->D: Compromises SIM1:ARNT2 heterodimer stability.
FT Does not compromises NPAS4:ARNT2 heterodimer stability."
FT /evidence="ECO:0000269|PubMed:27782878"
FT MUTAGEN 279
FT /note="V->D: Compromises SIM1:ARNT2 heterodimer stability.
FT Does not compromises NPAS4:ARNT2 heterodimer stability."
FT /evidence="ECO:0000269|PubMed:27782878"
FT MUTAGEN 340
FT /note="R->A: Compromises SIM1:ARNT2 heterodimer stability.
FT Does not compromises NPAS4:ARNT2 heterodimer stability."
FT /evidence="ECO:0000269|PubMed:27782878"
FT MUTAGEN 422
FT /note="N->A: Compromises SIM1:ARNT2 heterodimer stability.
FT Does not compromises NPAS4:ARNT2 heterodimer stability."
FT /evidence="ECO:0000269|PubMed:27782878"
FT CONFLICT 206
FT /note="M -> I (in Ref. 1; BAA09799)"
FT /evidence="ECO:0000305"
FT CONFLICT 432
FT /note="I -> T (in Ref. 3; AAH54546)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 712 AA; 77902 MW; FACD27033F7F18DE CRC64;
MATPAAVNPP EMASDIPGSV ALPVAPMAAT GQVRMAGAMP ARGGKRRSGM DFDDEDGEGP
SKFSRENHSE IERRRRNKMT QYITELSDMV PTCSALARKP DKLTILRMAV SHMKSMRGTG
NKSTDGAYKP SFLTEQELKH LILEAADGFL FVVAAETGRV IYVSDSVTPV LNQPQSEWFG
STLYEQVHPD DVEKLREQLC TSENSMTGRI LDLKTGTVKK EGQQSSMRMC MGSRRSFICR
MRCGNAPLDH LPLNRITTMR KRFRNGLGPV KEGEAQYAVV HCTGYIKAWP PAGMTIPEED
ADVGQGSKYC LVAIGRLQVT SSPVCMDMSG MSVPTEFLSR HNSDGIITFV DPRCISVIGY
QPQDLLGKDI LEFCHPEDQS HLRESFQQVV KLKGQVLSVM YRFRTKNREW LLIRTSSFTF
QNPYSDEIEY VICTNTNVKQ LQQQQAELEV HQRDGLSSYD LSQVPVPNLP AGVHEAGKSV
EKADAIFSQE RDPRFAEMFA GISASEKKMM SSASASGSQQ IYSQGSPFPA GHSGKAFSSS
VVHVPGVNDI QSSSSTGQNI SQISRQLNQG QVAWTGSRPP FPGQPSKTQS SAFGIGSSHP
YPADPSSYSP LSSPAASSPS GNAYPSLANR TPGFAESGQS GGQFQGRPSE VWSQWQSQHH
GQQSGEQHSH QQPGQTEVFQ DMLPMPGDPT QGTGNYNIED FADLGMFPPF SE
