ARNT_DROME
ID ARNT_DROME Reviewed; 642 AA.
AC O15945; O16167; O44082; Q24461; Q9VHI2;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2012, sequence version 3.
DT 03-AUG-2022, entry version 201.
DE RecName: Full=Aryl hydrocarbon receptor nuclear translocator homolog;
DE Short=dARNT;
DE AltName: Full=Hypoxia-inducible factor 1-beta;
DE AltName: Full=Protein tango;
GN Name=tgo; Synonyms=ARNT, HIF-1-beta; ORFNames=CG11987;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBUNIT.
RC TISSUE=Embryo;
RX PubMed=9374395; DOI=10.1242/dev.124.20.3975;
RA Ohshiro T., Saigo K.;
RT "Transcriptional regulation of breathless FGF receptor gene by binding of
RT TRACHEALESS/dARNT heterodimers to three central midline elements in
RT Drosophila developing trachea.";
RL Development 124:3975-3986(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, AND TISSUE SPECIFICITY.
RC TISSUE=Embryo;
RX PubMed=9284047; DOI=10.1101/gad.11.16.2079;
RA Zelzer E., Wappner P., Shilo B.-Z.;
RT "The PAS domain confers target gene specificity of Drosophila bHLH/PAS
RT proteins.";
RL Genes Dev. 11:2079-2089(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=9409674; DOI=10.1242/dev.124.22.4571;
RA Sonnenfeld M., Ward M., Nystroem G., Mosher J., Stahl S., Crews S.;
RT "The Drosophila tango gene encodes a bHLH-PAS protein that is orthologous
RT to mammalian Arnt and controls CNS midline and tracheal development.";
RL Development 124:4571-4582(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=Canton-S; TISSUE=Embryo, Head, and Pupae;
RX PubMed=10581393; DOI=10.1016/s0169-328x(99)00224-7;
RA Ma E., Haddad G.G.;
RT "Isolation and characterization of the hypoxia-inducible factor 1beta in
RT Drosophila melanogaster.";
RL Brain Res. Mol. Brain Res. 73:11-16(1999).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 586-623.
RX PubMed=2877746; DOI=10.1016/0092-8674(86)90516-7;
RA Frigerio G., Burri M., Bopp D., Baumgartner S., Noll M.;
RT "Structure of the segmentation gene paired and the Drosophila PRD gene set
RT as part of a gene network.";
RL Cell 47:735-746(1986).
CC -!- FUNCTION: Heterodimers of tgo/trh are involved in the control of
CC breathless expression. Plays a role in the cellular or tissue response
CC to oxygen deprivation. {ECO:0000269|PubMed:10581393,
CC ECO:0000269|PubMed:9284047, ECO:0000269|PubMed:9374395,
CC ECO:0000269|PubMed:9409674}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Heterodimer with ahr, trh or sim. {ECO:0000269|PubMed:9284047,
CC ECO:0000269|PubMed:9374395, ECO:0000269|PubMed:9409674}.
CC -!- INTERACTION:
CC O15945; P05709: sim; NbExp=3; IntAct=EBI-172695, EBI-88929;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: At stage 11, expression is detected in tracheal
CC pits. At later stages, strong expression is also detected in the CNS.
CC {ECO:0000269|PubMed:9284047, ECO:0000269|PubMed:9409674}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically in pupae
CC at a low level. {ECO:0000269|PubMed:10581393,
CC ECO:0000269|PubMed:9409674}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA28838.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAB69695.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB002556; BAA22868.1; -; mRNA.
DR EMBL; AF016053; AAB69695.1; ALT_FRAME; mRNA.
DR EMBL; AF020426; AAB88882.1; -; mRNA.
DR EMBL; AF154417; AAD38396.1; -; mRNA.
DR EMBL; AE014297; AAF54329.1; -; Genomic_DNA.
DR EMBL; AY069581; AAL39726.1; -; mRNA.
DR EMBL; M14550; AAA28838.1; ALT_FRAME; Genomic_DNA.
DR RefSeq; NP_001262397.1; NM_001275468.1.
DR RefSeq; NP_731308.1; NM_169254.2.
DR AlphaFoldDB; O15945; -.
DR SMR; O15945; -.
DR BioGRID; 66252; 19.
DR IntAct; O15945; 9.
DR STRING; 7227.FBpp0081483; -.
DR PaxDb; O15945; -.
DR PRIDE; O15945; -.
DR DNASU; 41084; -.
DR EnsemblMetazoa; FBtr0082005; FBpp0081483; FBgn0264075.
DR EnsemblMetazoa; FBtr0336755; FBpp0307731; FBgn0264075.
DR GeneID; 41084; -.
DR KEGG; dme:Dmel_CG11987; -.
DR CTD; 41084; -.
DR FlyBase; FBgn0264075; tgo.
DR VEuPathDB; VectorBase:FBgn0264075; -.
DR eggNOG; KOG3561; Eukaryota.
DR GeneTree; ENSGT00940000169286; -.
DR HOGENOM; CLU_011864_1_0_1; -.
DR InParanoid; O15945; -.
DR OMA; YARRELT; -.
DR OrthoDB; 331262at2759; -.
DR PhylomeDB; O15945; -.
DR Reactome; R-DME-1234158; Regulation of gene expression by Hypoxia-inducible Factor.
DR Reactome; R-DME-8937144; Aryl hydrocarbon receptor signalling.
DR SignaLink; O15945; -.
DR BioGRID-ORCS; 41084; 0 hits in 3 CRISPR screens.
DR ChiTaRS; tgo; fly.
DR GenomeRNAi; 41084; -.
DR PRO; PR:O15945; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0264075; Expressed in wing disc and 46 other tissues.
DR ExpressionAtlas; O15945; baseline and differential.
DR Genevisible; O15945; DM.
DR GO; GO:0034751; C:aryl hydrocarbon receptor complex; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0032991; C:protein-containing complex; IPI:FlyBase.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IMP:FlyBase.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0017022; F:myosin binding; IPI:FlyBase.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0048736; P:appendage development; IMP:FlyBase.
DR GO; GO:0007420; P:brain development; IMP:FlyBase.
DR GO; GO:0071456; P:cellular response to hypoxia; IDA:FlyBase.
DR GO; GO:0032869; P:cellular response to insulin stimulus; IDA:FlyBase.
DR GO; GO:0007417; P:central nervous system development; IMP:FlyBase.
DR GO; GO:0008347; P:glial cell migration; IMP:FlyBase.
DR GO; GO:0016348; P:imaginal disc-derived leg joint morphogenesis; IMP:FlyBase.
DR GO; GO:0008286; P:insulin receptor signaling pathway; IMP:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0045676; P:regulation of R7 cell differentiation; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:FlyBase.
DR CDD; cd00130; PAS; 2.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR001067; Nuc_translocat.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR013767; PAS_fold.
DR Pfam; PF00010; HLH; 1.
DR Pfam; PF00989; PAS; 1.
DR PRINTS; PR00785; NCTRNSLOCATR.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00091; PAS; 2.
DR SUPFAM; SSF47459; SSF47459; 1.
DR SUPFAM; SSF55785; SSF55785; 2.
DR TIGRFAMs; TIGR00229; sensory_box; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS50112; PAS; 2.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..642
FT /note="Aryl hydrocarbon receptor nuclear translocator
FT homolog"
FT /id="PRO_0000127117"
FT DOMAIN 13..66
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 85..156
FT /note="PAS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 271..341
FT /note="PAS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 346..389
FT /note="PAC"
FT REGION 450..612
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 450..485
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 500..560
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 110
FT /note="V -> M (in Ref. 1; BAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 410
FT /note="Q -> QQQ (in Ref. 2; AAB69695, 3; AAB88882 and 4;
FT AAD38396)"
FT /evidence="ECO:0000305"
FT CONFLICT 465
FT /note="G -> R (in Ref. 1; BAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 488
FT /note="P -> T (in Ref. 2; AAB69695)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 642 AA; 71475 MW; 0D2EB5D7651C98CC CRC64;
MDEANIQDKE RFASRENHCE IERRRRNKMT AYITELSDMV PTCSALARKP DKLTILRMAV
AHMKALRGTG NTSSDGTYKP SFLTDQELKH LILEAADGFL FVVSCDSGRV IYVSDSVTPV
LNYTQSDWYG TSLYEHIHPD DREKIREQLS TQESQNAGRI LDLKSGTVKK EGHQSSMRLS
MGARRGFICR MRVGNVNPES MVSGHLNRLK QRNSLGPSRD GTNYAVVHCT GYIKNWPPTD
MFPNMHMERD VDDMSSHCCL VAIGRLQVTS TAANDMSGSN NQSEFITRHA MDGKFTFVDQ
RVLNILGYTP TELLGKICYD FFHPEDQSHM KESFDQVLKQ KGQMFSLLYR ARAKNSEYVW
LRTQAYAFLN PYTDEVEYIV CTNSSGKTMH GAPLDAAAAH TPEQVQQQQQ QEQHVYVQAA
PGVDYARREL TPVGSATNDG MYQTHMLAMQ APTPQQQQQQ QQRPGSAQTT PVGYTYDTTH
SPYSAGGPSP LAKIPKSGTS PTPVAPNSWA ALRPQQQQQQ QQPVTEGYQY QQTSPARSPS
GPTYTQLSAG NGNRQQAQPG AYQAGPPPPP NAPGMWDWQQ AGGHPHPPHP TAHPHHPHAH
PGGPAGAGQP QGQEFSDMLQ MLDHTPTTFE DLNINMFSTP FE
