MRAZ_ECOLI
ID MRAZ_ECOLI Reviewed; 152 AA.
AC P22186;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Transcriptional regulator MraZ;
GN Name=mraZ {ECO:0000255|HAMAP-Rule:MF_01008}; Synonyms=yabB;
GN OrderedLocusNames=b0081, JW0079;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2187182; DOI=10.1093/nar/18.9.2813;
RA Gomez M.J., Fluoret B., van Heijenoort J., Ayala J.A.;
RT "Nucleotide sequence of the regulatory region of the gene pbpB of
RT Escherichia coli.";
RL Nucleic Acids Res. 18:2813-2813(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1630901; DOI=10.1093/nar/20.13.3305;
RA Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K.,
RA Mizobuchi K., Nakata A.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 0-
RT 2.4 min region.";
RL Nucleic Acids Res. 20:3305-3308(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION
RP PHENOTYPE, AND MUTAGENESIS OF ARG-15.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=24659771; DOI=10.1128/jb.01370-13;
RA Eraso J.M., Markillie L.M., Mitchell H.D., Taylor R.C., Orr G.,
RA Margolin W.;
RT "The highly conserved MraZ protein is a transcriptional regulator in
RT Escherichia coli.";
RL J. Bacteriol. 196:2053-2066(2014).
RN [6]
RP CRYSTALLIZATION, AND SUBUNIT.
RX PubMed=16511046; DOI=10.1107/s1744309105007657;
RA Adams M.A., Udell C.M., Pal G.P., Jia Z.;
RT "MraZ from Escherichia coli: cloning, purification, crystallization and
RT preliminary X-ray analysis.";
RL Acta Crystallogr. F 61:378-380(2005).
CC -!- FUNCTION: Negatively regulates its own expression and that of the
CC subsequent genes in the proximal part of the division and cell wall
CC (dcw) gene cluster. Acts by binding directly to DNA. May also regulate
CC the expression of genes outside the dcw cluster. {ECO:0000255|HAMAP-
CC Rule:MF_01008, ECO:0000269|PubMed:24659771}.
CC -!- SUBUNIT: Dodecamer. {ECO:0000269|PubMed:16511046}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid {ECO:0000255|HAMAP-
CC Rule:MF_01008, ECO:0000269|PubMed:24659771}.
CC -!- INDUCTION: Negatively autoregulated. {ECO:0000269|PubMed:24659771}.
CC -!- DISRUPTION PHENOTYPE: Mutants have no detectable phenotype under
CC standard laboratory conditions. They are more resistant to trimethoprim
CC than wild-type cells. {ECO:0000269|PubMed:24659771}.
CC -!- MISCELLANEOUS: Overproduction is lethal and perturbs cell division. It
CC destabilizes the divisome, but does not affect FtsZ levels or prevent Z
CC ring assembly. Co-overproduction of RsmH (MraW) protects the cell from
CC the toxic effects of MraZ overproduction (PubMed:24659771).
CC {ECO:0000305|PubMed:24659771}.
CC -!- SIMILARITY: Belongs to the MraZ family. {ECO:0000255|HAMAP-
CC Rule:MF_01008}.
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DR EMBL; X52063; CAA36284.1; -; Genomic_DNA.
DR EMBL; X55034; CAA38858.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73192.1; -; Genomic_DNA.
DR EMBL; AP009048; BAB96649.1; -; Genomic_DNA.
DR PIR; S14388; S14388.
DR RefSeq; NP_414623.1; NC_000913.3.
DR RefSeq; WP_001295533.1; NZ_STEB01000010.1.
DR AlphaFoldDB; P22186; -.
DR SMR; P22186; -.
DR BioGRID; 4261640; 156.
DR DIP; DIP-11166N; -.
DR IntAct; P22186; 5.
DR STRING; 511145.b0081; -.
DR jPOST; P22186; -.
DR PaxDb; P22186; -.
DR PRIDE; P22186; -.
DR EnsemblBacteria; AAC73192; AAC73192; b0081.
DR EnsemblBacteria; BAB96649; BAB96649; BAB96649.
DR GeneID; 944810; -.
DR KEGG; ecj:JW0079; -.
DR KEGG; eco:b0081; -.
DR PATRIC; fig|1411691.4.peg.2199; -.
DR EchoBASE; EB1076; -.
DR eggNOG; COG2001; Bacteria.
DR HOGENOM; CLU_107907_2_0_6; -.
DR InParanoid; P22186; -.
DR OMA; RGQERCL; -.
DR PhylomeDB; P22186; -.
DR BioCyc; EcoCyc:EG11084-MON; -.
DR PRO; PR:P22186; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR GO; GO:0009295; C:nucleoid; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISM:EcoCyc.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:EcoCyc.
DR GO; GO:2000143; P:negative regulation of DNA-templated transcription, initiation; IEP:EcoCyc.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEP:EcoCyc.
DR CDD; cd16321; MraZ_C; 1.
DR CDD; cd16320; MraZ_N; 1.
DR Gene3D; 3.40.1550.20; -; 1.
DR HAMAP; MF_01008; MraZ; 1.
DR InterPro; IPR003444; MraZ.
DR InterPro; IPR035644; MraZ_C.
DR InterPro; IPR020603; MraZ_dom.
DR InterPro; IPR035642; MraZ_N.
DR InterPro; IPR038619; MraZ_sf.
DR InterPro; IPR007159; SpoVT-AbrB_dom.
DR InterPro; IPR037914; SpoVT-AbrB_sf.
DR PANTHER; PTHR34701; PTHR34701; 1.
DR Pfam; PF02381; MraZ; 2.
DR SUPFAM; SSF89447; SSF89447; 1.
DR TIGRFAMs; TIGR00242; TIGR00242; 1.
DR PROSITE; PS51740; SPOVT_ABRB; 2.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..152
FT /note="Transcriptional regulator MraZ"
FT /id="PRO_0000108479"
FT DOMAIN 5..52
FT /note="SpoVT-AbrB 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01076"
FT DOMAIN 81..124
FT /note="SpoVT-AbrB 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01076"
FT MUTAGEN 15
FT /note="R->A: Abolishes DNA binding."
FT /evidence="ECO:0000269|PubMed:24659771"
SQ SEQUENCE 152 AA; 17386 MW; 3EE1A6FA9D2B1C01 CRC64;
MFRGATLVNL DSKGRLSVPT RYREQLLENA AGQMVCTIDI YHPCLLLYPL PEWEIIEQKL
SRLSSMNPVE RRVQRLLLGH ASECQMDGAG RLLIAPVLRQ HAGLTKEVML VGQFNKFELW
DETTWHQQVK EDIDAEQLAT GDLSERLQDL SL
