MRE11_ARATH
ID MRE11_ARATH Reviewed; 720 AA.
AC Q9XGM2;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Double-strand break repair protein MRE11;
DE Short=AtMre11;
GN Name=MRE11; OrderedLocusNames=At5g54260 {ECO:0000312|Araport:AT5G54260};
GN ORFNames=MDK4.8 {ECO:0000312|EMBL:BAB10749.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Hartung F., Puchta H.;
RT "Isolation of the complete cDNA of the Mre11 homologue of Arabidopsis
RT indicates conservation of DNA recombination mechanisms between plants and
RT other eucaryotes.";
RL (er) Plant Gene Register PGR99-132(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP REVIEW ON DNA REPAIR.
RX PubMed=16202663; DOI=10.1016/j.dnarep.2005.08.017;
RA Bleuyard J.Y., Gallego M.E., White C.I.;
RT "Recent advances in understanding of the DNA double-strand break repair
RT machinery of plants.";
RL DNA Repair 5:1-12(2006).
RN [5]
RP INTERACTION WITH MRE11 AND RAD50.
RC STRAIN=cv. Columbia;
RX PubMed=17672843; DOI=10.1111/j.1365-313x.2007.03220.x;
RA Waterworth W.M., Altun C., Armstrong S.J., Roberts N., Dean P.J., Young K.,
RA Weil C.F., Bray C.M., West C.E.;
RT "NBS1 is involved in DNA repair and plays a synergistic role with ATM in
RT mediating meiotic homologous recombination in plants.";
RL Plant J. 52:41-52(2007).
CC -!- FUNCTION: Involved in DNA double-strand break repair (DSBR). Possesses
CC single-strand endonuclease activity and double-strand-specific 3'-5'
CC exonuclease activity. Also involved in meiotic DSB processing (By
CC similarity). {ECO:0000250|UniProtKB:P32829}.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000250|UniProtKB:Q8U1N9};
CC Note=Binds 2 manganese ions per subunit.
CC {ECO:0000250|UniProtKB:Q8U1N9};
CC -!- SUBUNIT: Component of the MRN complex composed of two heterodimers
CC RAD50/MRE11 associated with a single NBN (By similarity). Interacts
CC with NBS1 and RAD50. {ECO:0000250|UniProtKB:Q09683,
CC ECO:0000269|PubMed:17672843}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q23255}.
CC -!- SIMILARITY: Belongs to the MRE11/RAD32 family. {ECO:0000305}.
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DR EMBL; AJ243822; CAB50793.1; -; mRNA.
DR EMBL; AB010695; BAB10749.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96476.1; -; Genomic_DNA.
DR PIR; T52564; T52564.
DR RefSeq; NP_200237.1; NM_124806.2.
DR AlphaFoldDB; Q9XGM2; -.
DR SMR; Q9XGM2; -.
DR BioGRID; 20758; 19.
DR STRING; 3702.AT5G54260.1; -.
DR iPTMnet; Q9XGM2; -.
DR PaxDb; Q9XGM2; -.
DR PRIDE; Q9XGM2; -.
DR ProteomicsDB; 239077; -.
DR EnsemblPlants; AT5G54260.1; AT5G54260.1; AT5G54260.
DR GeneID; 835514; -.
DR Gramene; AT5G54260.1; AT5G54260.1; AT5G54260.
DR KEGG; ath:AT5G54260; -.
DR Araport; AT5G54260; -.
DR TAIR; locus:2162615; AT5G54260.
DR eggNOG; KOG2310; Eukaryota.
DR HOGENOM; CLU_009535_3_2_1; -.
DR InParanoid; Q9XGM2; -.
DR OMA; QWMRPET; -.
DR PhylomeDB; Q9XGM2; -.
DR PRO; PR:Q9XGM2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9XGM2; baseline and differential.
DR Genevisible; Q9XGM2; AT.
DR GO; GO:0000785; C:chromatin; IDA:TAIR.
DR GO; GO:0000791; C:euchromatin; IDA:TAIR.
DR GO; GO:0030870; C:Mre11 complex; IBA:GO_Central.
DR GO; GO:0035861; C:site of double-strand break; IBA:GO_Central.
DR GO; GO:0000795; C:synaptonemal complex; IDA:TAIR.
DR GO; GO:0008296; F:3'-5'-exodeoxyribonuclease activity; IEA:InterPro.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IBA:GO_Central.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IBA:GO_Central.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IBA:GO_Central.
DR GO; GO:0097552; P:mitochondrial double-strand break repair via homologous recombination; IBA:GO_Central.
DR GO; GO:0007095; P:mitotic G2 DNA damage checkpoint signaling; IBA:GO_Central.
DR GO; GO:0000723; P:telomere maintenance; IBA:GO_Central.
DR CDD; cd00840; MPP_Mre11_N; 1.
DR Gene3D; 3.30.110.110; -; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR InterPro; IPR003701; Mre11.
DR InterPro; IPR038487; Mre11_capping_dom.
DR InterPro; IPR007281; Mre11_DNA-bd.
DR InterPro; IPR041796; Mre11_N.
DR Pfam; PF00149; Metallophos; 1.
DR Pfam; PF04152; Mre11_DNA_bind; 1.
DR PIRSF; PIRSF000882; DSB_repair_MRE11; 1.
DR SMART; SM01347; Mre11_DNA_bind; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
DR TIGRFAMs; TIGR00583; mre11; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA repair; Endonuclease; Exonuclease; Hydrolase; Manganese;
KW Meiosis; Metal-binding; Nuclease; Nucleus; Reference proteome.
FT CHAIN 1..720
FT /note="Double-strand break repair protein MRE11"
FT /id="PRO_0000138679"
FT REGION 503..720
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 508..568
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 582..611
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 630..653
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 654..669
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 125
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q8U1N9"
FT BINDING 17
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q8U1N9"
FT BINDING 19
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q8U1N9"
FT BINDING 57
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q8U1N9"
FT BINDING 57
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q8U1N9"
FT BINDING 124
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q8U1N9"
FT BINDING 250
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q8U1N9"
FT BINDING 252
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q8U1N9"
SQ SEQUENCE 720 AA; 80291 MW; DA5F0CAA46D8780C CRC64;
MSREDFSDTL RVLVATDCHL GYMEKDEIRR HDSFKAFEEI CSIAEEKQVD FLLLGGDLFH
ENKPSRTTLV KAIEILRRHC LNDKPVQFQV VSDQTVNFQN AFGQVNYEDP HFNVGLPVFS
IHGNHDDPAG VDNLSAIDIL SACNLVNYFG KMVLGGSGVG QITLYPILMK KGSTTVALYG
LGNIRDERLN RMFQTPHAVQ WMRPEVQEGC DVSDWFNILV LHQNRVKSNP KNAISEHFLP
RFLDFIVWGH EHECLIDPQE VSGMGFHITQ PGSSVATSLI DGESKPKHVL LLEIKGNQYR
PTKIPLTSVR PFEYTEIVLK DESDIDPNDQ NSILEHLDKV VRNLIEKASK KAVNRSEIKL
PLVRIKVDYS GFMTINPQRF GQKYVGKVAN PQDILIFSKA SKKGRSEANI DDSERLRPEE
LNQQNIEALV AESNLKMEIL PVNDLDVALH NFVNKDDKLA FYSCVQYNLQ ETRGKLAKDS
DAKKFEEDDL ILKVGECLEE RLKDRSTRPT GSSQFLSTGL TSENLTKGSS GIANASFSDD
EDTTQMSGLA PPTRGRRGSS TANTTRGRAK APTRGRGRGK ASSAMKQTTL DSSLGFRQSQ
RSASAAASAA FKSASTIGED DVDSPSSEEV EPEDFNKPDS SSEDDESTKG KGRKRPATTK
RGRGRGSGTS KRGRKNESSS SLNRLLSSKD DDEDEDDEDR EKKLNKSQPR VTRNYGALRR
