MRE11_CAEEL
ID MRE11_CAEEL Reviewed; 728 AA.
AC Q23255;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Double-strand break repair protein mre-11;
GN Name=mre-11 {ECO:0000312|WormBase:ZC302.1};
GN ORFNames=ZC302.1 {ECO:0000312|WormBase:ZC302.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP MUTAGENESIS OF GLU-95.
RX PubMed=11238374; DOI=10.1101/gad.864101;
RA Chin G.M., Villeneuve A.M.;
RT "C. elegans mre-11 is required for meiotic recombination and DNA repair but
RT is dispensable for the meiotic G(2) DNA damage checkpoint.";
RL Genes Dev. 15:522-534(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16628214; DOI=10.1038/sj.emboj.7601102;
RA Polanowska J., Martin J.S., Garcia-Muse T., Petalcorin M.I.R.,
RA Boulton S.J.;
RT "A conserved pathway to activate BRCA1-dependent ubiquitylation at DNA
RT damage sites.";
RL EMBO J. 25:2178-2188(2006).
CC -!- FUNCTION: Involved in DNA double-strand break repair (DSBR)
CC (PubMed:11238374). Possesses single-strand endonuclease activity and
CC double-strand-specific 3'-5' exonuclease activity (PubMed:11238374).
CC Also involved in meiotic DSB processing (PubMed:11238374). In response
CC to ionizing radiation, probably required for the association between
CC the brc-1-brd-1 heterodimer and rad-51 and let-70 in order to activate
CC E3-ubiquitin ligase activity of the heterodimer and induce
CC ubiquitination at DNA damage sites (PubMed:16628214).
CC {ECO:0000269|PubMed:11238374, ECO:0000269|PubMed:16628214}.
CC -!- FUNCTION: Required for meiotic crossing over and chiasma formation.
CC Pachytene morphology and homolog pairing are normal. Vital in long term
CC for maintenance of reproductive capacity of subsequent generations.
CC {ECO:0000269|PubMed:11238374}.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000250|UniProtKB:P32829};
CC -!- SUBUNIT: Forms a complex with rad-50. {ECO:0000250|UniProtKB:P32829}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11238374}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in DNA damage
CC repair defects following ionizing radiation with reduced ubiquitination
CC at DNA damage sites. {ECO:0000269|PubMed:16628214}.
CC -!- SIMILARITY: Belongs to the MRE11/RAD32 family.
CC {ECO:0000255|RuleBase:RU003447, ECO:0000269|PubMed:11238374}.
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DR EMBL; Z73978; CAA98292.2; -; Genomic_DNA.
DR PIR; T27512; T27512.
DR RefSeq; NP_505736.2; NM_073335.4.
DR AlphaFoldDB; Q23255; -.
DR SMR; Q23255; -.
DR BioGRID; 44517; 17.
DR IntAct; Q23255; 3.
DR MINT; Q23255; -.
DR STRING; 6239.ZC302.1.1; -.
DR iPTMnet; Q23255; -.
DR EPD; Q23255; -.
DR PaxDb; Q23255; -.
DR PeptideAtlas; Q23255; -.
DR EnsemblMetazoa; ZC302.1.1; ZC302.1.1; WBGene00003405.
DR EnsemblMetazoa; ZC302.1.2; ZC302.1.2; WBGene00003405.
DR GeneID; 179488; -.
DR KEGG; cel:CELE_ZC302.1; -.
DR UCSC; ZC302.1.1; c. elegans.
DR CTD; 179488; -.
DR WormBase; ZC302.1; CE37459; WBGene00003405; mre-11.
DR eggNOG; KOG2310; Eukaryota.
DR GeneTree; ENSGT00390000017288; -.
DR HOGENOM; CLU_009535_3_0_1; -.
DR InParanoid; Q23255; -.
DR OMA; PSREVQH; -.
DR OrthoDB; 834694at2759; -.
DR PhylomeDB; Q23255; -.
DR Reactome; R-CEL-1834949; Cytosolic sensors of pathogen-associated DNA.
DR Reactome; R-CEL-2559586; DNA Damage/Telomere Stress Induced Senescence.
DR Reactome; R-CEL-5685939; HDR through MMEJ (alt-NHEJ).
DR Reactome; R-CEL-5693548; Sensing of DNA Double Strand Breaks.
DR Reactome; R-CEL-5693565; Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
DR Reactome; R-CEL-5693607; Processing of DNA double-strand break ends.
DR PRO; PR:Q23255; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00003405; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0030870; C:Mre11 complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0035861; C:site of double-strand break; IBA:GO_Central.
DR GO; GO:0008296; F:3'-5'-exodeoxyribonuclease activity; IEA:InterPro.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IBA:GO_Central.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IBA:GO_Central.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IBA:GO_Central.
DR GO; GO:0097552; P:mitochondrial double-strand break repair via homologous recombination; IBA:GO_Central.
DR GO; GO:0007095; P:mitotic G2 DNA damage checkpoint signaling; IBA:GO_Central.
DR GO; GO:0031573; P:mitotic intra-S DNA damage checkpoint signaling; IBA:GO_Central.
DR GO; GO:0000723; P:telomere maintenance; IBA:GO_Central.
DR CDD; cd00840; MPP_Mre11_N; 1.
DR Gene3D; 3.30.110.110; -; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR InterPro; IPR003701; Mre11.
DR InterPro; IPR038487; Mre11_capping_dom.
DR InterPro; IPR007281; Mre11_DNA-bd.
DR InterPro; IPR041796; Mre11_N.
DR Pfam; PF00149; Metallophos; 1.
DR Pfam; PF04152; Mre11_DNA_bind; 1.
DR PIRSF; PIRSF000882; DSB_repair_MRE11; 1.
DR SMART; SM01347; Mre11_DNA_bind; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
DR TIGRFAMs; TIGR00583; mre11; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA repair; Endonuclease; Exonuclease; Hydrolase; Manganese;
KW Meiosis; Nuclease; Nucleus; Reference proteome.
FT CHAIN 1..728
FT /note="Double-strand break repair protein mre-11"
FT /id="PRO_0000138678"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 601..728
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..38
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 619..636
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 673..694
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 182
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT MUTAGEN 95
FT /note="E->K: In mre-11-ME41; defective in meiotic
FT chromosome degradation."
FT /evidence="ECO:0000269|PubMed:11238374"
SQ SEQUENCE 728 AA; 81559 MW; E86C5BEF81216DC9 CRC64;
MCGSDDSFDD FVPDSQEPAS SRTRNQDHLD DDEVPCSQRP DAANDTMVED LDEGDEPAHD
ESEDIIKILV ATDIHCGYGE NKANIHMDAV NTFEEVLQIA TEQKVDMILL GGDLFHENNP
SREVQHRVTQ LLRQYCLNGN PIALEFLSDA SVNFNQSVFG HVNYYDQNLN VGLPIFTIHG
NHDDLSGKGL TALDLLHESG LVNLFGKHSN IQEFIVSPIL LRKGETRLAL YGIGSQRDDR
LVRAFKNNSI SFLRPNAGAE DWFNLFVLHQ NRPRRAMHRS TGNFLPESLI PQFFDLLIWG
HEHECKPDPQ YVASSEAVGD GFYILQPGST VATSLTPEEA LQKNAFLIKI KGRKFASKPI
PLQTVRPMVC DELLLDKIPP GSRPILKTDR PKHTDGRYID EIAIEAKINE MITTAKAKRR
PRQPELPLIR LKVIYDGDWL NITPANAKRI GLRYENVVAN AVDMVFIKKN NKPKEGKLQT
ENEKNITEMA DEMGQVSATN LQTIINDYFI NQPLVDQMTV LKPIGIGRAL EQYSAIEEGG
LATSANRNFD SCLMHQIDVV RKTLKKMPLP PINDPSDLDK FRDLITKDLY DMKKADSERF
KNPVADVEME EDEDDPQYYM PPQSTSRTNY ASGSEDEVAN SDEEMGSSIS RHSKQPTTRG
RGRGARGAGA SRETTRGRSN KVVSTRQIDS DGFEIINDSP SPPPASRSTR GKARGKSAPS
KKRDLSFF
