MRF2_ARATH
ID MRF2_ARATH Reviewed; 693 AA.
AC O80548; O64378;
DT 03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 141.
DE RecName: Full=MA3 DOMAIN-CONTAINING TRANSLATION REGULATORY FACTOR 2 {ECO:0000303|PubMed:29084871};
DE AltName: Full=MA3 domain-containing protein 8 {ECO:0000303|PubMed:24041411};
GN Name=MRF2 {ECO:0000303|PubMed:29084871};
GN Synonyms=MAT8 {ECO:0000303|PubMed:24041411};
GN OrderedLocusNames=At1g22730 {ECO:0000312|Araport:AT1G22730};
GN ORFNames=T22J18.10 {ECO:0000312|EMBL:AAC25511.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 79-693.
RC STRAIN=cv. Columbia;
RX PubMed=9369203; DOI=10.1016/s0014-5793(97)01190-3;
RA Terryn N., Neyt P., de Clercq R., de Keyser A., van den Daele H.,
RA Ardiles W., Dehais P., Rouze P., Gielen J., Villarroel R., van Montagu M.;
RT "Sequence analysis of a 24-kb contiguous genomic region at the Arabidopsis
RT thaliana PFL locus on chromosome 1.";
RL FEBS Lett. 416:156-160(1997).
RN [5]
RP GENE FAMILY.
RX PubMed=24041411; DOI=10.1186/1471-2148-13-199;
RA Cheng S., Liu R., Gallie D.R.;
RT "The unique evolution of the programmed cell death 4 protein in plants.";
RL BMC Evol. Biol. 13:199-199(2013).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY DARK AND STARVATION, TISSUE
RP SPECIFICITY, INTERACTION WITH EIF4A1 AND RIBOSOMES, SUBCELLULAR LOCATION,
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=29084871; DOI=10.1105/tpc.17.00563;
RA Lee D.-H., Park S.J., Ahn C.S., Pai H.-S.;
RT "MRF family genes are involved in translation control, especially under
RT energy-deficient conditions, and their expression and functions are
RT modulated by the TOR signaling pathway.";
RL Plant Cell 29:2895-2920(2017).
CC -!- FUNCTION: Involved in target of rapamycin (TOR)-regulated translation
CC control, especially under energy-deficient conditions.
CC {ECO:0000269|PubMed:29084871}.
CC -!- SUBUNIT: Binds to EIF4A1 (PubMed:29084871). The association with
CC ribosomes is modulated by cellular energy status and TOR activity
CC (PubMed:29084871). {ECO:0000269|PubMed:29084871}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC Cytoplasm, cytosol {ECO:0000269|PubMed:29084871}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in reproductive tissues, such as
CC flower buds and flowers, and, to a lower extent, in vegetative tissues,
CC such as leaves, roots and stems. {ECO:0000269|PubMed:29084871}.
CC -!- INDUCTION: Induced by dark and starvation.
CC {ECO:0000269|PubMed:29084871}.
CC -!- DISRUPTION PHENOTYPE: Increased susceptibility to dark and starvation,
CC and to treatment with the TOR inhibitor (PubMed:29084871). Decreased
CC translation activity associated with altered ribosome patterns,
CC especially in the dark and starvation conditions, in which mRNAs
CC distribution is altered and rRNA abnormally degraded (PubMed:29084871).
CC Slightly early flowering time under long-day conditions
CC (PubMed:29084871). {ECO:0000269|PubMed:29084871}.
CC -!- SIMILARITY: Belongs to the PDCD4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA72903.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC003979; AAC25511.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30278.1; -; Genomic_DNA.
DR EMBL; AY034972; AAK59477.1; -; mRNA.
DR EMBL; AY133820; AAM91754.1; -; mRNA.
DR EMBL; Y12227; CAA72903.1; ALT_FRAME; Genomic_DNA.
DR PIR; T00771; T00771.
DR RefSeq; NP_173687.1; NM_102120.3.
DR AlphaFoldDB; O80548; -.
DR SMR; O80548; -.
DR STRING; 3702.AT1G22730.1; -.
DR PaxDb; O80548; -.
DR PRIDE; O80548; -.
DR ProteomicsDB; 183755; -.
DR EnsemblPlants; AT1G22730.1; AT1G22730.1; AT1G22730.
DR GeneID; 838879; -.
DR Gramene; AT1G22730.1; AT1G22730.1; AT1G22730.
DR KEGG; ath:AT1G22730; -.
DR Araport; AT1G22730; -.
DR TAIR; locus:2199695; AT1G22730.
DR eggNOG; KOG0403; Eukaryota.
DR HOGENOM; CLU_013764_1_0_1; -.
DR InParanoid; O80548; -.
DR OMA; KEACRCI; -.
DR OrthoDB; 434771at2759; -.
DR PhylomeDB; O80548; -.
DR PRO; PR:O80548; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O80548; baseline and differential.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0043022; F:ribosome binding; IDA:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR GO; GO:0009646; P:response to absence of light; IEP:TAIR.
DR GO; GO:0090549; P:response to carbon starvation; IEP:TAIR.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003891; Initiation_fac_eIF4g_MI.
DR InterPro; IPR039778; PDCD4.
DR PANTHER; PTHR12626; PTHR12626; 1.
DR Pfam; PF02847; MA3; 4.
DR SMART; SM00544; MA3; 4.
DR SUPFAM; SSF48371; SSF48371; 4.
DR PROSITE; PS51366; MI; 4.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; Repeat; Translation regulation.
FT CHAIN 1..693
FT /note="MA3 DOMAIN-CONTAINING TRANSLATION REGULATORY FACTOR
FT 2"
FT /id="PRO_0000447575"
FT DOMAIN 90..211
FT /note="MI 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT DOMAIN 254..375
FT /note="MI 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT DOMAIN 389..510
FT /note="MI 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT DOMAIN 560..681
FT /note="MI 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT REGION 25..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 673..693
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 241..248
FT /note="Nuclear localization signal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT MOTIF 430..437
FT /note="Nuclear localization signal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 37..54
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 693 AA; 77244 MW; D5EE92BEEC190C15 CRC64;
MEHQDLTDSR KDPLCISQLK ISSSSLDPLP QANMAEDLTK SRRHSPIKVE GSEETWGVED
DDDLTDPIFD TIEGNGHSDP TSCFDADLSE YKKKATVIVE EYFGTNDVVS VVNELKELGM
AEYRYYFVKK LVSMAMDRHD KEKEMAAFLL STLYADVIDP PEVYRGFNKL VASADDLSVD
IPDAVDVLAV FVARAIVDDI LPPAFLKKQM KLLPDNSKGV EVLRKAEKSY LATPLHAEVV
EKRWGGTDNW TAEDVKARIN DLLKEYVMSG DKKEAFRCIK GLKVPFFHHE IVKRALIMAM
ERRKAQVRLL DLLKETIEVG LINSTQVTKG FSRIIDSIED LSLDIPDARR ILQSFISKAA
SEGWLCASSL KSLSADAGEK LLENSSANVF KDKAKSIIRE YFLSGDTSEV VHCLDTELNA
SSSQLRAIFV KYLITLAMDR KKREKEMACV LVSTLGFPPK DVRSAFSMLI ESADDTALDN
PVVVEDLAMF LARAVVDEVL APRDLEEVLN QTPEAGSSVG EKVIQMAKTL LKARLSGERI
LRCWGGGGIE TNSPGSTVKE VKEKIQILLE EYVSGGDLRE ASRCVKELGM PFFHHEVVKK
SVVRIIEEKE NEERLWKLLK VCFDSGLVTI YQMTKGFKRV DESLEDLSLD VPDAAKKFSS
CVERGKLEGF LDESFASEDS QSKKQNGSSS SSG
