MRF4_ARATH
ID MRF4_ARATH Reviewed; 633 AA.
AC Q9STL9; A0A178VI37;
DT 03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=MA3 DOMAIN-CONTAINING TRANSLATION REGULATORY FACTOR 4 {ECO:0000303|PubMed:29084871};
DE AltName: Full=MA3 domain-containing protein 7 {ECO:0000303|PubMed:24041411};
GN Name=MRF4 {ECO:0000303|PubMed:29084871};
GN Synonyms=MAT7 {ECO:0000303|PubMed:24041411};
GN OrderedLocusNames=At3g48390 {ECO:0000312|Araport:AT3G48390};
GN ORFNames=T29H11_90 {ECO:0000312|EMBL:CAB41159.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=24041411; DOI=10.1186/1471-2148-13-199;
RA Cheng S., Liu R., Gallie D.R.;
RT "The unique evolution of the programmed cell death 4 protein in plants.";
RL BMC Evol. Biol. 13:199-199(2013).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY DARK AND STARVATION, TISSUE
RP SPECIFICITY, INTERACTION WITH EIF4A1 AND RIBOSOMES, SUBCELLULAR LOCATION,
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=29084871; DOI=10.1105/tpc.17.00563;
RA Lee D.-H., Park S.J., Ahn C.S., Pai H.-S.;
RT "MRF family genes are involved in translation control, especially under
RT energy-deficient conditions, and their expression and functions are
RT modulated by the TOR signaling pathway.";
RL Plant Cell 29:2895-2920(2017).
CC -!- FUNCTION: Involved in target of rapamycin (TOR)-regulated translation
CC control, especially under energy-deficient conditions.
CC {ECO:0000269|PubMed:29084871}.
CC -!- SUBUNIT: Binds to EIF4A1 (PubMed:29084871). The association with
CC ribosomes is modulated by cellular energy status and TOR activity
CC (PubMed:29084871). {ECO:0000269|PubMed:29084871}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC Cytoplasm, cytosol {ECO:0000269|PubMed:29084871}.
CC -!- TISSUE SPECIFICITY: Mostly expressed, at low levels, in rosette leaves
CC and flower buds, and, to a lower extent, in roots, stems, cauline
CC leaves and flowers. {ECO:0000269|PubMed:29084871}.
CC -!- INDUCTION: Induced by dark and starvation but repressed by glucose
CC feeding subsequent to starvation in a TOR-dependent manner.
CC {ECO:0000269|PubMed:29084871}.
CC -!- DISRUPTION PHENOTYPE: Increased susceptibility to dark and starvation,
CC and to treatment with the TOR inhibitor (PubMed:29084871). Decreased
CC translation activity associated with altered ribosome patterns,
CC especially in the dark and starvation conditions, in which mRNAs
CC distribution is altered and rRNA abnormally degraded (PubMed:29084871).
CC Slightly early flowering time under long-day conditions
CC (PubMed:29084871). {ECO:0000269|PubMed:29084871}.
CC -!- SIMILARITY: Belongs to the PDCD4 family. {ECO:0000305}.
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DR EMBL; AL049659; CAB41159.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78411.1; -; Genomic_DNA.
DR EMBL; AY074567; AAL67107.1; -; mRNA.
DR EMBL; BT002208; AAN72220.1; -; mRNA.
DR PIR; T06703; T06703.
DR RefSeq; NP_190411.1; NM_114699.5.
DR AlphaFoldDB; Q9STL9; -.
DR SMR; Q9STL9; -.
DR STRING; 3702.AT3G48390.1; -.
DR iPTMnet; Q9STL9; -.
DR PaxDb; Q9STL9; -.
DR PRIDE; Q9STL9; -.
DR ProteomicsDB; 189372; -.
DR DNASU; 823997; -.
DR EnsemblPlants; AT3G48390.1; AT3G48390.1; AT3G48390.
DR GeneID; 823997; -.
DR Gramene; AT3G48390.1; AT3G48390.1; AT3G48390.
DR KEGG; ath:AT3G48390; -.
DR Araport; AT3G48390; -.
DR TAIR; locus:2101155; AT3G48390.
DR eggNOG; KOG0403; Eukaryota.
DR HOGENOM; CLU_013764_1_0_1; -.
DR InParanoid; Q9STL9; -.
DR OMA; HAEENGW; -.
DR OrthoDB; 434771at2759; -.
DR PhylomeDB; Q9STL9; -.
DR PRO; PR:Q9STL9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9STL9; baseline and differential.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0043022; F:ribosome binding; IDA:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR GO; GO:0009646; P:response to absence of light; IEP:TAIR.
DR GO; GO:0090549; P:response to carbon starvation; IEP:TAIR.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003891; Initiation_fac_eIF4g_MI.
DR InterPro; IPR039778; PDCD4.
DR PANTHER; PTHR12626; PTHR12626; 1.
DR Pfam; PF02847; MA3; 4.
DR SMART; SM00544; MA3; 4.
DR SUPFAM; SSF48371; SSF48371; 4.
DR PROSITE; PS51366; MI; 4.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; Repeat; Translation regulation.
FT CHAIN 1..633
FT /note="MA3 DOMAIN-CONTAINING TRANSLATION REGULATORY FACTOR
FT 4"
FT /id="PRO_0000447577"
FT DOMAIN 56..177
FT /note="MI 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT DOMAIN 220..341
FT /note="MI 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT DOMAIN 351..472
FT /note="MI 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT DOMAIN 514..633
FT /note="MI 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT MOTIF 94..101
FT /note="Nuclear localization signal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT MOTIF 389..396
FT /note="Nuclear localization signal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
SQ SEQUENCE 633 AA; 70752 MW; E7D0FDDBCF5E2198 CRC64;
MATREWFVMN AQQTKLALES PPLFAVKKDD DKDHTCDTGE EPYALVGSPV FNPLEDYKRE
VVSIIDEYFS SGDVEVAASD LMDLGLSEYH PYFVKRLVSM AMDRGNKEKE KASVLLSRLY
ALVVSPDQIR VGFIRLLESV GDLALDIPDA VNVLALFIAR AIVDEILPPV FLARAKKTLP
HSSQGFQVIL VSENSYLSAP HHAELVETKW GGSTHITVEE TKRKISEFLN EYVENGDTRE
ACRCIRELGV SFFHHEIVKS GLVLVMESRT SEPLILKLLK EATEEGLISS SQMAKGFSRV
ADSLDDLSLD IPSAKTLFES IVPKAIIGGW LDEDSFKERS DQNGGSENLR RFKKDAETII
QEYFLSDDIP ELIRSLEDLG LPEYNPVFLK KLITLAMDRK NKEKEMASVF LASLHMEMFS
TEDFINGFIM LLESAEDTAL DILAASDELA LFLARAVIDD VLAPLNLEEI SNSLPPKSTG
SETIRSARSL ISARHAGERL LRSWGGGTGW AVEDAKDKIW KLLEEYEVGG VISEACRCIR
DLGMPFFNHE VVKKALVMAM EKKNDRMLNL LQECFAEGII TTNQMTKGFG RVKDSLDDLS
LDIPNAEEKF NSYVAHAEEN GWLHRDFGCS TDS
