MRFA_DICDI
ID MRFA_DICDI Reviewed; 932 AA.
AC Q54PT9; Q94474;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Myelin gene regulatory factor-like A;
GN Name=mrfA; Synonyms=rcdK; ORFNames=DDB_G0284183;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 283-889.
RC STRAIN=AX4;
RA Loomis W.F.;
RL Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP DEVELOPMENTAL STAGE.
RX PubMed=12796308; DOI=10.1128/ec.2.3.627-637.2003;
RA Maeda M., Sakamoto H., Iranfar N., Fuller D., Maruo T., Ogihara S.,
RA Morio T., Urushihara H., Tanaka Y., Loomis W.F.;
RT "Changing patterns of gene expression in Dictyostelium prestalk cell
RT subtypes recognized by in situ hybridization with genes from microarray
RT analyses.";
RL Eukaryot. Cell 2:627-637(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ARG-376 AND ARG-460, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=22811266; DOI=10.1387/ijdb.120030jw;
RA Senoo H., Wang H.Y., Araki T., Williams J.G., Fukuzawa M.;
RT "An orthologue of the myelin-gene regulatory transcription factor regulates
RT Dictyostelium prestalk differentiation.";
RL Int. J. Dev. Biol. 56:325-332(2012).
CC -!- FUNCTION: Transcription factor which acts as key regulator of pstA
CC (prestalk-A) cells differentiation. Essential for ecmA-specific gene
CC expression. {ECO:0000269|PubMed:22811266}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Do not show cell-type-specific expression.
CC {ECO:0000269|PubMed:12796308}.
CC -!- DISRUPTION PHENOTYPE: Null cells are delayed in multicellular
CC development and highly defective in pstA-specific gene expression.
CC {ECO:0000269|PubMed:22811266}.
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DR EMBL; AAFI02000064; EAL65280.1; -; Genomic_DNA.
DR EMBL; U66369; AAB06761.1; -; mRNA.
DR RefSeq; XP_638706.1; XM_633614.1.
DR AlphaFoldDB; Q54PT9; -.
DR SMR; Q54PT9; -.
DR STRING; 44689.DDB0191426; -.
DR PaxDb; Q54PT9; -.
DR EnsemblProtists; EAL65280; EAL65280; DDB_G0284183.
DR GeneID; 8624537; -.
DR KEGG; ddi:DDB_G0284183; -.
DR dictyBase; DDB_G0284183; mrfA.
DR eggNOG; KOG3661; Eukaryota.
DR HOGENOM; CLU_314093_0_0_1; -.
DR InParanoid; Q54PT9; -.
DR OMA; EYCIVSY; -.
DR PhylomeDB; Q54PT9; -.
DR PRO; PR:Q54PT9; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005737; C:cytoplasm; IDA:dictyBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IDA:dictyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:dictyBase.
DR GO; GO:0016540; P:protein autoprocessing; IBA:GO_Central.
DR GO; GO:0031285; P:regulation of sorocarp stalk cell differentiation; IMP:dictyBase.
DR Gene3D; 2.60.40.1390; -; 1.
DR InterPro; IPR024061; NDT80_DNA-bd_dom.
DR InterPro; IPR037141; NDT80_DNA-bd_dom_sf.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR030392; S74_ICA.
DR Pfam; PF05224; NDT80_PhoG; 1.
DR Pfam; PF13884; Peptidase_S74; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS51688; ICA; 1.
DR PROSITE; PS51517; NDT80; 1.
PE 1: Evidence at protein level;
KW Coiled coil; DNA-binding; Membrane; Reference proteome; Transcription;
KW Transcription regulation; Transmembrane; Transmembrane helix.
FT CHAIN 1..932
FT /note="Myelin gene regulatory factor-like A"
FT /id="PRO_0000371411"
FT TRANSMEM 895..915
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 767..877
FT /note="Peptidase S74"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01025"
FT DNA_BIND 286..546
FT /note="NDT80"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00850"
FT REGION 1..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 152..256
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 269..328
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 540..568
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 582..601
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 613..660
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 680..726
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 127..154
FT /evidence="ECO:0000255"
FT COILED 863..895
FT /evidence="ECO:0000255"
FT COMPBIAS 307..328
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 552..568
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 376
FT /note="R->A: Loss of binding to ecmA promoter."
FT /evidence="ECO:0000269|PubMed:22811266"
FT MUTAGEN 460
FT /note="R->A: Loss of binding to ecmA promoter."
FT /evidence="ECO:0000269|PubMed:22811266"
FT CONFLICT 641
FT /note="N -> NN (in Ref. 2; AAB06761)"
FT /evidence="ECO:0000305"
FT CONFLICT 829..830
FT /note="NA -> KT (in Ref. 2; AAB06761)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 932 AA; 104316 MW; 79BF650ECE483438 CRC64;
MDGYNQQQQQ QQQQQQQHQM NLGSSGGILH SPQQQQQQQQ QQQQQQQQPM NGSNNQLLGI
SNGGFGTMLQ QSASLPINIQ GSNSFSTFTP LSADDSNLKK RKIKNEDGTY SEIPYSPNSL
VGSNGGLDSS FLMLQQQLQD QQQQIAQFNS SVNSPSFSLN SSQVNNNNNN NNNINNNNNS
NNNINNNNSN NNINNNNNNN NNNNNNINNN NINNNNNYNN NNNNNNHNHL QTTTTTTTNN
GLTINANNTP SQLSSSSLYP GIIPQISITN TQSGYTPRSI SPNPSPNLSP TNSPIQSPHL
PTDNYLMIGS ENENSDPPSP MTQYNENPPF TWTDYKSEPW YTTFNTNGDE MPVPLLNIMA
SKGFSYIGGQ WIYCRRNHFQ LDITAVYPKL FQETQYGHNG VTLSNSSSSV DPTQTPSYML
ISGVKTPING LTLTIKGIKN RADMSQQESE VELFQTNSKR EKQGEHAPKP VAIQFGSLVS
IQRLHFRKAT LNNARRHGQP NPHQEFNQLV VSLYGRCMGQ EYCIVSYVSP ALIVRTATQV
TPPGDLSPVH TPDYAGTSSN NGGGSNNMNC FGPTISNIDF STMNIVSPGH SPTSPRPLSQ
LTSQGQTNIL RLTQNHNNNN NNNNNNNNNN NNNNNNNNNN NINNNNNSNN NSLGNINNNN
SNMLTSSSLL NGRINCFNNN INNNNNNQHN NNNQHNNNNN HHNINNQHNN QNNNNNNNNN
NKCHWNSGDT EKSIVYNGKV GINVENPAYA LSVQGTIYAS EGVYHPSDLR IKYDLKSIDS
KSNLDNVNRM KLYDYKYNPQ WTHMNGRDPY LDNCDRGVIA QDLQRILPNA VRTIGNKNVN
GQEIENLLVI KNEALVMETI GATQELSKQM DEMKLKLITY ESKLKNLKKK SKNQTILLII
FMITFLLVAL YMYKPSTHHS HHRRHNFDNN DN
