MRGA1_MOUSE
ID MRGA1_MOUSE Reviewed; 304 AA.
AC Q91WW5; A0JNS0;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Mas-related G-protein coupled receptor member A1;
DE AltName: Full=RF-amide G-protein coupled receptor;
GN Name=Mrgpra1; Synonyms=Mrga1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Spinal ganglion;
RX PubMed=11551509; DOI=10.1016/s0092-8674(01)00483-4;
RA Dong X., Han S.-K., Zylka M.J., Simon M.I., Anderson D.J.;
RT "A diverse family of GPCRs expressed in specific subsets of nociceptive
RT sensory neurons.";
RL Cell 106:619-632(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION.
RX PubMed=12397184; DOI=10.1073/pnas.192565799;
RA Han S.-K., Dong X., Hwang J.-I., Zylka M.J., Anderson D.J., Simon M.I.;
RT "Orphan G protein-coupled receptors MrgA1 and MrgC11 are distinctively
RT activated by RF-amide-related peptides through the Galpha q/11 pathway.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:14740-14745(2002).
CC -!- FUNCTION: Orphan receptor activated by a subset of RFamide-family
CC neuropeptides such as FLRF-amide and FMRF-amide. Mediates its action by
CC association with G proteins that activate a phosphatidylinositol-
CC calcium second messenger system. Its effect is mediated by G(q) and
CC G(11) proteins. May regulate the function of nociceptive neurons by
CC modulation of pain perception. {ECO:0000269|PubMed:12397184}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11551509};
CC Multi-pass membrane protein {ECO:0000269|PubMed:11551509}.
CC -!- TISSUE SPECIFICITY: Expressed in a subset of sensory neurons that
CC includes nociceptors. Expressed in the subclass of non-peptidergic
CC sensory neurons that are IB4(+) and VR1(-).
CC {ECO:0000269|PubMed:11551509}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Mas
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AY042191; AAK91787.1; -; mRNA.
DR EMBL; BC126873; AAI26874.1; -; mRNA.
DR RefSeq; NP_694735.2; NM_153095.2.
DR AlphaFoldDB; Q91WW5; -.
DR SMR; Q91WW5; -.
DR STRING; 10090.ENSMUSP00000129978; -.
DR ChEMBL; CHEMBL4523413; -.
DR GlyGen; Q91WW5; 1 site.
DR PaxDb; Q91WW5; -.
DR PRIDE; Q91WW5; -.
DR DNASU; 233221; -.
DR GeneID; 233221; -.
DR KEGG; mmu:233221; -.
DR CTD; 233221; -.
DR MGI; MGI:3033095; Mrgpra1.
DR eggNOG; ENOG502RTWA; Eukaryota.
DR InParanoid; Q91WW5; -.
DR OrthoDB; 1123658at2759; -.
DR PhylomeDB; Q91WW5; -.
DR BioGRID-ORCS; 233221; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Mrgpra1; mouse.
DR PRO; PR:Q91WW5; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q91WW5; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISO:MGI.
DR GO; GO:0042923; F:neuropeptide binding; ISO:MGI.
DR GO; GO:0008188; F:neuropeptide receptor activity; IDA:MGI.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; ISO:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0045576; P:mast cell activation; ISO:MGI.
DR GO; GO:0043303; P:mast cell degranulation; ISO:MGI.
DR GO; GO:0032467; P:positive regulation of cytokinesis; ISO:MGI.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR026233; MRGPCRA.
DR InterPro; IPR026234; MRGPCRFAMILY.
DR PANTHER; PTHR11334; PTHR11334; 1.
DR PANTHER; PTHR11334:SF33; PTHR11334:SF33; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR02109; MRGPCRA.
DR PRINTS; PR02108; MRGPCRFAMILY.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..304
FT /note="Mas-related G-protein coupled receptor member A1"
FT /id="PRO_0000069747"
FT TOPO_DOM 1..17
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 18..38
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 39..53
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 75
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 97..131
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..166
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..206
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..243
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 265..304
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 10
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 12
FT /note="T -> R (in Ref. 2; AAI26874)"
FT /evidence="ECO:0000305"
FT CONFLICT 32
FT /note="G -> A (in Ref. 2; AAI26874)"
FT /evidence="ECO:0000305"
FT CONFLICT 43
FT /note="H -> R (in Ref. 2; AAI26874)"
FT /evidence="ECO:0000305"
FT CONFLICT 88
FT /note="T -> I (in Ref. 2; AAI26874)"
FT /evidence="ECO:0000305"
FT CONFLICT 166
FT /note="N -> S (in Ref. 2; AAI26874)"
FT /evidence="ECO:0000305"
FT CONFLICT 189
FT /note="A -> S (in Ref. 2; AAI26874)"
FT /evidence="ECO:0000305"
FT CONFLICT 208
FT /note="I -> M (in Ref. 2; AAI26874)"
FT /evidence="ECO:0000305"
FT CONFLICT 293
FT /note="I -> N (in Ref. 2; AAI26874)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 304 AA; 34381 MW; C56CBF879067A52B CRC64;
MDNTIPGGIN ITILIPNLMI IIFGLVGLTG NGIVFWLLGF CLHRNAFSVY ILNLALADFF
FLLGHIIDSI LLLLNVFYPI TFLLCFYTIM MVLYIAGLSM LSAISTERCL SVLCPIWYHC
HRPEHTSTVM CAVIWVLSLL ICILNSYFCG FLNTQYKNEN GCLALNFFTA AYLMFLFVVL
CLSSLALVAR LFCGTGQIKL TRLYVTIILS ILVFLLCGLP FGIHWFLLFK IKDDFHVFDL
GFYLASVVLT AINSCANPII YFFVGSFRHR LKHQTLKMVL QNALQDTPET AKIMVEMSRS
KSEP
