MRGA3_MOUSE
ID MRGA3_MOUSE Reviewed; 302 AA.
AC Q91WW3;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 132.
DE RecName: Full=Mas-related G-protein coupled receptor member A3;
GN Name=Mrgpra3; Synonyms=Mrga3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Spinal ganglion;
RX PubMed=11551509; DOI=10.1016/s0092-8674(01)00483-4;
RA Dong X., Han S.-K., Zylka M.J., Simon M.I., Anderson D.J.;
RT "A diverse family of GPCRs expressed in specific subsets of nociceptive
RT sensory neurons.";
RL Cell 106:619-632(2001).
RN [2]
RP TISSUE SPECIFICITY, AND FUNCTION AS ITCH RECEPTOR.
RX PubMed=20004959; DOI=10.1016/j.cell.2009.11.034;
RA Liu Q., Tang Z., Surdenikova L., Kim S., Patel K.N., Kim A., Ru F.,
RA Guan Y., Weng H.J., Geng Y., Undem B.J., Kollarik M., Chen Z.F.,
RA Anderson D.J., Dong X.;
RT "Sensory neuron-specific GPCR Mrgprs are itch receptors mediating
RT chloroquine-induced pruritus.";
RL Cell 139:1353-1365(2009).
CC -!- FUNCTION: Orphan receptor. May be a receptor for RFamide-family
CC neuropeptides such as NPFF and NPAF, which are analgesic in vivo. May
CC regulate nociceptor function and/or development, including the
CC sensation or modulation of pain (By similarity). Activated by the
CC antimalarial drug chloroquine. Mediates chloroquine-induced itch, in a
CC histamine-independent manner. {ECO:0000250,
CC ECO:0000269|PubMed:20004959}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed exclusively in dorsal root ganglia and
CC nodose ganglia. Expressed in a subset of sensory neurons that includes
CC nociceptors. Expressed in the subclass of non-peptidergic sensory
CC neurons that are IB4(+) and VR1(-). {ECO:0000269|PubMed:11551509,
CC ECO:0000269|PubMed:20004959}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Mas
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AY042193; AAK91789.1; -; mRNA.
DR RefSeq; NP_694707.2; NM_153067.2.
DR AlphaFoldDB; Q91WW3; -.
DR SMR; Q91WW3; -.
DR STRING; 10090.ENSMUSP00000135437; -.
DR GlyGen; Q91WW3; 3 sites.
DR PaxDb; Q91WW3; -.
DR PRIDE; Q91WW3; -.
DR DNASU; 233222; -.
DR GeneID; 233222; -.
DR KEGG; mmu:233222; -.
DR CTD; 233222; -.
DR MGI; MGI:2684085; Mrgpra3.
DR eggNOG; ENOG502RTWA; Eukaryota.
DR InParanoid; Q91WW3; -.
DR OrthoDB; 1123658at2759; -.
DR PhylomeDB; Q91WW3; -.
DR BioGRID-ORCS; 233222; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q91WW3; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q91WW3; protein.
DR GO; GO:0030424; C:axon; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0032809; C:neuronal cell body membrane; IDA:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISO:MGI.
DR GO; GO:0042923; F:neuropeptide binding; ISO:MGI.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IMP:UniProtKB.
DR GO; GO:0006953; P:acute-phase response; IEA:UniProtKB-KW.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IMP:UniProtKB.
DR GO; GO:0007635; P:chemosensory behavior; IDA:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0045576; P:mast cell activation; ISO:MGI.
DR GO; GO:0043303; P:mast cell degranulation; ISO:MGI.
DR GO; GO:0032467; P:positive regulation of cytokinesis; ISO:MGI.
DR GO; GO:1902349; P:response to chloroquine; IMP:UniProtKB.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR026233; MRGPCRA.
DR InterPro; IPR026234; MRGPCRFAMILY.
DR PANTHER; PTHR11334; PTHR11334; 1.
DR PANTHER; PTHR11334:SF33; PTHR11334:SF33; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR02109; MRGPCRA.
DR PRINTS; PR02108; MRGPCRFAMILY.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Acute phase; Cell membrane; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..302
FT /note="Mas-related G-protein coupled receptor member A3"
FT /id="PRO_0000069749"
FT TOPO_DOM 1..17
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 18..38
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 39..46
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..81
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..129
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..167
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..211
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..242
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..302
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 2
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 68
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 159
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 302 AA; 34483 MW; 8E4CC023ACA2E4E5 CRC64;
MNETIPGSID IETLIPDLMI IIFGLVGLTG NAIVFWLLGF RMHRTAFLVY ILNLALADFL
FLLCHIINST VDLLKFTLPK GIFAFCFHTI KRVLYITGLS MLSAISTERC LSVLCPIWYH
CRRPEHTSTV MCAVIWVLSL LICILDGYFC GYLDNHYFNY SVCQAWDIFI GAYLMFLFVV
LCLSTLALLA RLFCGARNMK FTRLFVTIML TVLVFLLCGL PWGITWFLLF WIAPGVFVLD
YSPLLVLTAI NSCANPIIYF FVGSFRQRLN KQTLKMVLQK ALQDTPETPE NMVEMSRNKA
EP
