MRGA4_MOUSE
ID MRGA4_MOUSE Reviewed; 313 AA.
AC Q91WW2; Q1RLL1;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2013, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Mas-related G-protein coupled receptor member A4;
DE AltName: Full=RF-amide G-protein coupled receptor;
GN Name=Mrgpra4; Synonyms=Mrga4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Spinal ganglion;
RX PubMed=11551509; DOI=10.1016/s0092-8674(01)00483-4;
RA Dong X., Han S.-K., Zylka M.J., Simon M.I., Anderson D.J.;
RT "A diverse family of GPCRs expressed in specific subsets of nociceptive
RT sensory neurons.";
RL Cell 106:619-632(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Orphan receptor. May be a receptor for RFamide-family
CC neuropeptides such as NPFF and NPAF, which are analgesic in vivo. May
CC regulate nociceptor function and/or development, including the
CC sensation or modulation of pain.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11551509};
CC Multi-pass membrane protein {ECO:0000269|PubMed:11551509}.
CC -!- TISSUE SPECIFICITY: Expressed in a subset of sensory neurons that
CC includes nociceptors. Expressed in the subclass of non-peptidergic
CC sensory neurons that are IB4(+) and VR1(-).
CC {ECO:0000269|PubMed:11551509}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Mas
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AY042194; AAK91790.1; -; mRNA.
DR EMBL; AC144923; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466603; EDL22963.1; -; Genomic_DNA.
DR EMBL; BC115741; AAI15742.1; -; mRNA.
DR CCDS; CCDS21298.1; -.
DR RefSeq; NP_705744.2; NM_153524.2.
DR AlphaFoldDB; Q91WW2; -.
DR SMR; Q91WW2; -.
DR STRING; 10090.ENSMUSP00000084327; -.
DR GlyGen; Q91WW2; 2 sites.
DR PaxDb; Q91WW2; -.
DR PRIDE; Q91WW2; -.
DR DNASU; 235854; -.
DR Ensembl; ENSMUST00000087092; ENSMUSP00000084327; ENSMUSG00000067173.
DR GeneID; 235854; -.
DR KEGG; mmu:235854; -.
DR UCSC; uc009hak.1; mouse.
DR CTD; 235854; -.
DR MGI; MGI:3033100; Mrgpra4.
DR VEuPathDB; HostDB:ENSMUSG00000067173; -.
DR eggNOG; ENOG502RTWA; Eukaryota.
DR GeneTree; ENSGT01030000234639; -.
DR HOGENOM; CLU_009579_4_1_1; -.
DR InParanoid; Q91WW2; -.
DR OMA; FIITAWL; -.
DR OrthoDB; 1123658at2759; -.
DR PhylomeDB; Q91WW2; -.
DR TreeFam; TF336336; -.
DR BioGRID-ORCS; 235854; 4 hits in 72 CRISPR screens.
DR PRO; PR:Q91WW2; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q91WW2; protein.
DR ExpressionAtlas; Q91WW2; baseline and differential.
DR Genevisible; Q91WW2; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISO:MGI.
DR GO; GO:0042923; F:neuropeptide binding; ISO:MGI.
DR GO; GO:0008188; F:neuropeptide receptor activity; IDA:MGI.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; ISO:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0045576; P:mast cell activation; ISO:MGI.
DR GO; GO:0043303; P:mast cell degranulation; ISO:MGI.
DR GO; GO:0032467; P:positive regulation of cytokinesis; ISO:MGI.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR026233; MRGPCRA.
DR InterPro; IPR026234; MRGPCRFAMILY.
DR PANTHER; PTHR11334; PTHR11334; 1.
DR PANTHER; PTHR11334:SF33; PTHR11334:SF33; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR02109; MRGPCRA.
DR PRINTS; PR02108; MRGPCRFAMILY.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..313
FT /note="Mas-related G-protein coupled receptor member A4"
FT /id="PRO_0000069750"
FT TOPO_DOM 1..25
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 26..46
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 47..54
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..93
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 115..137
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..182
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 204..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..255
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..313
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 10
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 76
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 204
FT /note="A -> G (in Ref. 1; AAK91790)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 313 AA; 35682 MW; A428C370BBD0F202 CRC64;
MAPTTTNPMN ETIPGSIDIE TLIPNLMIII FGLVGLTGNV ILFWLLGFHL HRNAFLVYIL
NLALADFLFL LCHIINSTML LLKVHLPNNI LNHCFDIIMT VLYITGLSML SAISTERCLS
VLCPIWYRCR RPEHTSTVLC AVIWFLPLLI CILNGYFCHF FGPKYVIDSV CLATNFFIRT
YPMFLFIVLC LSTLALLARL FCGAGKTKFT RLFVTIMLTV LVFLLCGLPL GFFWFLVPWI
NRDFSVLDYI LFQTSLVLTS VNSCANPIIY FFVGSFRHRL KHKTLKMVLQ SALQDTPETP
ENMVEMSRSK AEP
