MRGRF_HUMAN
ID MRGRF_HUMAN Reviewed; 343 AA.
AC Q96AM1; B3KV43; Q8NBK8;
DT 01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Mas-related G-protein coupled receptor member F;
DE Short=Mas-related gene F protein;
DE AltName: Full=G-protein coupled receptor 140;
DE AltName: Full=G-protein coupled receptor 168;
GN Name=MRGPRF; Synonyms=GPR140, GPR168, MRGF; ORFNames=PSEC0142;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Uterus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ARG-16.
RC TISSUE=Placenta;
RX PubMed=16303743; DOI=10.1093/dnares/12.2.117;
RA Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J.,
RA Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S.,
RA Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.,
RA Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S.,
RA Isogai T.;
RT "Signal sequence and keyword trap in silico for selection of full-length
RT human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA
RT libraries.";
RL DNA Res. 12:117-126(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Orphan receptor. May bind to a neuropeptide and may regulate
CC nociceptor function and/or development, including the sensation or
CC modulation of pain (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Mas
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AK075450; BAC11628.1; -; mRNA.
DR EMBL; AK122669; BAG53655.1; -; mRNA.
DR EMBL; CH471076; EAW74730.1; -; Genomic_DNA.
DR EMBL; BC016964; AAH16964.1; -; mRNA.
DR CCDS; CCDS8188.1; -.
DR RefSeq; NP_001091985.1; NM_001098515.1.
DR RefSeq; NP_659452.3; NM_145015.4.
DR RefSeq; XP_016872659.1; XM_017017170.1.
DR AlphaFoldDB; Q96AM1; -.
DR SMR; Q96AM1; -.
DR BioGRID; 125520; 2.
DR IntAct; Q96AM1; 4.
DR MINT; Q96AM1; -.
DR STRING; 9606.ENSP00000309782; -.
DR ChEMBL; CHEMBL4523903; -.
DR TCDB; 9.A.14.13.7; the g-protein-coupled receptor (gpcr) family.
DR GlyGen; Q96AM1; 1 site.
DR iPTMnet; Q96AM1; -.
DR PhosphoSitePlus; Q96AM1; -.
DR BioMuta; MRGPRF; -.
DR DMDM; 24638055; -.
DR jPOST; Q96AM1; -.
DR MassIVE; Q96AM1; -.
DR PaxDb; Q96AM1; -.
DR PeptideAtlas; Q96AM1; -.
DR PRIDE; Q96AM1; -.
DR ProteomicsDB; 75974; -.
DR Antibodypedia; 16748; 282 antibodies from 30 providers.
DR DNASU; 116535; -.
DR Ensembl; ENST00000309099.7; ENSP00000309782.6; ENSG00000172935.9.
DR Ensembl; ENST00000441623.1; ENSP00000403660.1; ENSG00000172935.9.
DR GeneID; 116535; -.
DR KEGG; hsa:116535; -.
DR MANE-Select; ENST00000309099.7; ENSP00000309782.6; NM_145015.5; NP_659452.3.
DR UCSC; uc001ooo.5; human.
DR CTD; 116535; -.
DR DisGeNET; 116535; -.
DR GeneCards; MRGPRF; -.
DR HGNC; HGNC:24828; MRGPRF.
DR HPA; ENSG00000172935; Tissue enhanced (endometrium).
DR MIM; 607233; gene.
DR neXtProt; NX_Q96AM1; -.
DR OpenTargets; ENSG00000172935; -.
DR PharmGKB; PA134896544; -.
DR VEuPathDB; HostDB:ENSG00000172935; -.
DR eggNOG; ENOG502SIXZ; Eukaryota.
DR GeneTree; ENSGT01030000234639; -.
DR HOGENOM; CLU_009579_4_1_1; -.
DR InParanoid; Q96AM1; -.
DR OMA; RILGLCM; -.
DR OrthoDB; 835806at2759; -.
DR PhylomeDB; Q96AM1; -.
DR TreeFam; TF336336; -.
DR PathwayCommons; Q96AM1; -.
DR SignaLink; Q96AM1; -.
DR BioGRID-ORCS; 116535; 17 hits in 1066 CRISPR screens.
DR ChiTaRS; MRGPRF; human.
DR GeneWiki; MRGPRF; -.
DR GenomeRNAi; 116535; -.
DR Pharos; Q96AM1; Tbio.
DR PRO; PR:Q96AM1; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q96AM1; protein.
DR Bgee; ENSG00000172935; Expressed in mucosa of stomach and 151 other tissues.
DR ExpressionAtlas; Q96AM1; baseline and differential.
DR Genevisible; Q96AM1; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031965; C:nuclear membrane; IDA:HPA.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR026228; MRGPCRF.
DR InterPro; IPR026234; MRGPCRFAMILY.
DR PANTHER; PTHR11334; PTHR11334; 1.
DR PANTHER; PTHR11334:SF3; PTHR11334:SF3; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR02112; MRGPCRF.
DR PRINTS; PR02108; MRGPCRFAMILY.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..343
FT /note="Mas-related G-protein coupled receptor member F"
FT /id="PRO_0000069764"
FT TOPO_DOM 1..44
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..66
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..104
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 105..123
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..160
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 182..198
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..220
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 221..241
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..263
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..273
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..343
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 320..343
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 325..343
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 4
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 16
FT /note="K -> R (in dbSNP:rs11544721)"
FT /evidence="ECO:0000269|PubMed:16303743"
FT /id="VAR_061220"
SQ SEQUENCE 343 AA; 38171 MW; 168A649F5B38F154 CRC64;
MAGNCSWEAH PGNRNKMCPG LSEAPELYSR GFLTIEQIAM LPPPAVMNYI FLLLCLCGLV
GNGLVLWFFG FSIKRNPFSI YFLHLASADV GYLFSKAVFS ILNTGGFLGT FADYIRSVCR
VLGLCMFLTG VSLLPAVSAE RCASVIFPAW YWRRRPKRLS AVVCALLWVL SLLVTCLHNY
FCVFLGRGAP GAACRHMDIF LGILLFLLCC PLMVLPCLAL ILHVECRARR RQRSAKLNHV
ILAMVSVFLV SSIYLGIDWF LFWVFQIPAP FPEYVTDLCI CINSSAKPIV YFLAGRDKSQ
RLWEPLRVVF QRALRDGAEL GEAGGSTPNT VTMEMQCPPG NAS
