MRGX3_HUMAN
ID MRGX3_HUMAN Reviewed; 322 AA.
AC Q96LB0; B0M0L1; Q8TDE0; Q8TDE1;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Mas-related G-protein coupled receptor member X3;
DE AltName: Full=Sensory neuron-specific G-protein coupled receptor 1/2;
GN Name=MRGPRX3; Synonyms=MRGX3, SNSR1, SNSR2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT ASP-169.
RX PubMed=11551509; DOI=10.1016/s0092-8674(01)00483-4;
RA Dong X., Han S.-K., Zylka M.J., Simon M.I., Anderson D.J.;
RT "A diverse family of GPCRs expressed in specific subsets of nociceptive
RT sensory neurons.";
RL Cell 106:619-632(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND VARIANT ASP-169.
RX PubMed=11850634; DOI=10.1038/nn815;
RA Lembo P.M.C., Grazzini E., Groblewski T., O'Donnell D., Roy M.-O.,
RA Zhang J., Hoffert C., Cao J., Schmidt R., Pelletier M., Labarre M.,
RA Gosselin M., Fortin Y., Banville D., Shen S., Stroem P., Payza K., Dray A.,
RA Walker P., Ahmad S.;
RT "Proenkephalin A gene products activate a new family of sensory neuron-
RT specific GPCRs.";
RL Nat. Neurosci. 5:201-209(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ASP-169.
RC TISSUE=Brain;
RA Martin A.L., Kaighin V.A., Aronstam R.S.;
RL Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT ASP-169.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS ARG-82 AND ASP-169.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Orphan receptor. Probably involved in the function of
CC nociceptive neurons. May regulate nociceptor function and/or
CC development, including the sensation or modulation of pain. Potently
CC activated by enkephalins (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q96LB0; Q5BVD1: TTMP; NbExp=3; IntAct=EBI-17958655, EBI-10243654;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Uniquely localized in a subset of small dorsal root
CC and trigeminal sensory neurons. {ECO:0000269|PubMed:11850634}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Mas
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AY042215; AAK91806.1; -; Genomic_DNA.
DR EMBL; AF474987; AAL86878.2; -; Genomic_DNA.
DR EMBL; AF474988; AAL86879.2; -; Genomic_DNA.
DR EMBL; EU432126; ABY87925.1; -; mRNA.
DR EMBL; AC090099; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471064; EAW68417.1; -; Genomic_DNA.
DR EMBL; BC067292; AAH67292.1; -; mRNA.
DR CCDS; CCDS7830.1; -.
DR RefSeq; NP_473372.3; NM_054031.3.
DR RefSeq; XP_011518184.1; XM_011519882.2.
DR AlphaFoldDB; Q96LB0; -.
DR SMR; Q96LB0; -.
DR BioGRID; 125576; 1.
DR IntAct; Q96LB0; 1.
DR STRING; 9606.ENSP00000379571; -.
DR ChEMBL; CHEMBL4523905; -.
DR BioMuta; MRGPRX3; -.
DR DMDM; 311033400; -.
DR PaxDb; Q96LB0; -.
DR PRIDE; Q96LB0; -.
DR Antibodypedia; 24979; 184 antibodies from 25 providers.
DR DNASU; 117195; -.
DR Ensembl; ENST00000396275.2; ENSP00000379571.2; ENSG00000179826.8.
DR Ensembl; ENST00000621697.2; ENSP00000481943.1; ENSG00000179826.8.
DR GeneID; 117195; -.
DR KEGG; hsa:117195; -.
DR MANE-Select; ENST00000621697.2; ENSP00000481943.1; NM_001370464.1; NP_001357393.1.
DR UCSC; uc001mnu.3; human.
DR CTD; 117195; -.
DR DisGeNET; 117195; -.
DR GeneCards; MRGPRX3; -.
DR HGNC; HGNC:17980; MRGPRX3.
DR HPA; ENSG00000179826; Tissue enhanced (esophagus, salivary gland).
DR MIM; 607229; gene.
DR neXtProt; NX_Q96LB0; -.
DR OpenTargets; ENSG00000179826; -.
DR PharmGKB; PA142671336; -.
DR VEuPathDB; HostDB:ENSG00000179826; -.
DR eggNOG; ENOG502RTWA; Eukaryota.
DR GeneTree; ENSGT01030000234639; -.
DR HOGENOM; CLU_009579_4_1_1; -.
DR InParanoid; Q96LB0; -.
DR OMA; CHVHLIS; -.
DR OrthoDB; 1123658at2759; -.
DR PhylomeDB; Q96LB0; -.
DR TreeFam; TF336336; -.
DR PathwayCommons; Q96LB0; -.
DR SignaLink; Q96LB0; -.
DR BioGRID-ORCS; 117195; 34 hits in 1015 CRISPR screens.
DR GeneWiki; MRGPRX3; -.
DR GenomeRNAi; 117195; -.
DR Pharos; Q96LB0; Tdark.
DR PRO; PR:Q96LB0; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q96LB0; protein.
DR Bgee; ENSG00000179826; Expressed in minor salivary gland and 11 other tissues.
DR Genevisible; Q96LB0; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR026234; MRGPCRFAMILY.
DR PANTHER; PTHR11334; PTHR11334; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR02108; MRGPCRFAMILY.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; G-protein coupled receptor; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..322
FT /note="Mas-related G-protein coupled receptor member X3"
FT /id="PRO_0000069782"
FT TOPO_DOM 1..31
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..60
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 82..96
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 118..140
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..177
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..213
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 235..254
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..322
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VARIANT 82
FT /note="C -> R (in dbSNP:rs12291017)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_025507"
FT VARIANT 169
FT /note="N -> D (in dbSNP:rs4274188)"
FT /evidence="ECO:0000269|PubMed:11551509,
FT ECO:0000269|PubMed:11850634, ECO:0000269|PubMed:15489334,
FT ECO:0000269|Ref.3, ECO:0000269|Ref.5"
FT /id="VAR_019434"
FT CONFLICT 3
FT /note="S -> P (in Ref. 2; AAL86878/AAL86879)"
FT /evidence="ECO:0000305"
FT CONFLICT 5
FT /note="I -> V (in Ref. 2; AAL86879)"
FT /evidence="ECO:0000305"
FT CONFLICT 91
FT /note="R -> S (in Ref. 2; AAL86879)"
FT /evidence="ECO:0000305"
FT CONFLICT 114
FT /note="S -> N (in Ref. 2; AAL86879)"
FT /evidence="ECO:0000305"
FT CONFLICT 149
FT /note="L -> P (in Ref. 2; AAL86879)"
FT /evidence="ECO:0000305"
FT CONFLICT 172
FT /note="W -> R (in Ref. 2; AAL86879)"
FT /evidence="ECO:0000305"
FT CONFLICT 188
FT /note="C -> R (in Ref. 2; AAL86879)"
FT /evidence="ECO:0000305"
FT CONFLICT 275
FT /note="V -> M (in Ref. 2; AAL86879)"
FT /evidence="ECO:0000305"
FT CONFLICT 281
FT /note="R -> L (in Ref. 2; AAL86879)"
FT /evidence="ECO:0000305"
FT CONFLICT 285..286
FT /note="QN -> KT (in Ref. 2; AAL86879)"
FT /evidence="ECO:0000305"
FT CONFLICT 294
FT /note="A -> D (in Ref. 2; AAL86879)"
FT /evidence="ECO:0000305"
FT CONFLICT 306
FT /note="G -> W (in Ref. 2; AAL86879)"
FT /evidence="ECO:0000305"
FT CONFLICT 307
FT /note="W -> Q (in Ref. 5; AAH67292)"
FT /evidence="ECO:0000305"
FT CONFLICT 319
FT /note="R -> K (in Ref. 2; AAL86878)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 322 AA; 36483 MW; CBDF55AF4C1EEB7E CRC64;
MDSTIPVLGT ELTPINGREE TPCYKQTLSF TGLTCIVSLV ALTGNAVVLW LLGCRMRRNA
VSIYILNLVA ADFLFLSGHI ICSPLRLINI RHPISKILSP VMTFPYFIGL SMLSAISTER
CLSILWPIWY HCRRPRYLSS VMCVLLWALS LLRSILEWMF CDFLFSGANS VWCETSDFIT
IAWLVFLCVV LCGSSLVLLV RILCGSRKMP LTRLYVTILL TVLVFLLCGL PFGIQWALFS
RIHLDWKVLF CHVHLVSIFL SALNSSANPI IYFFVGSFRQ RQNRQNLKLV LQRALQDTPE
VDEGGGWLPQ ETLELSGSRL EQ
