MRGX4_HUMAN
ID MRGX4_HUMAN Reviewed; 322 AA.
AC Q96LA9; Q3KNU3; Q3KNU4; Q502W0; Q8TDD6; Q8TDD7;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 08-FEB-2011, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Mas-related G-protein coupled receptor member X4;
DE AltName: Full=Sensory neuron-specific G-protein coupled receptor 5/6;
GN Name=MRGPRX4; Synonyms=MRGX4, SNSR5, SNSR6;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT SER-83.
RX PubMed=11551509; DOI=10.1016/s0092-8674(01)00483-4;
RA Dong X., Han S.-K., Zylka M.J., Simon M.I., Anderson D.J.;
RT "A diverse family of GPCRs expressed in specific subsets of nociceptive
RT sensory neurons.";
RL Cell 106:619-632(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LEU-8; LYS-25; CYS-54; SER-83
RP AND VAL-182, AND TISSUE SPECIFICITY.
RX PubMed=11850634; DOI=10.1038/nn815;
RA Lembo P.M.C., Grazzini E., Groblewski T., O'Donnell D., Roy M.-O.,
RA Zhang J., Hoffert C., Cao J., Schmidt R., Pelletier M., Labarre M.,
RA Gosselin M., Fortin Y., Banville D., Shen S., Stroem P., Payza K., Dray A.,
RA Walker P., Ahmad S.;
RT "Proenkephalin A gene products activate a new family of sensory neuron-
RT specific GPCRs.";
RL Nat. Neurosci. 5:201-209(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS SER-83 AND VAL-182.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Orphan receptor. Probably involved in the function of
CC nociceptive neurons. May regulate nociceptor function and/or
CC development, including the sensation or modulation of pain. Potently
CC activated by enkephalins (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Uniquely localized in a subset of small dorsal root
CC and trigeminal sensory neurons. {ECO:0000269|PubMed:11850634}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Mas
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AY042216; AAK91807.1; -; Genomic_DNA.
DR EMBL; AF474991; AAL86882.1; -; Genomic_DNA.
DR EMBL; AF474992; AAL86883.1; -; Genomic_DNA.
DR EMBL; AC090099; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC095509; AAH95509.1; -; mRNA.
DR EMBL; BC107099; AAI07100.1; -; mRNA.
DR EMBL; BC107100; AAI07101.1; -; mRNA.
DR CCDS; CCDS7831.1; -.
DR RefSeq; NP_473373.2; NM_054032.3.
DR PDB; 7S8P; EM; 2.60 A; R=2-322.
DR PDBsum; 7S8P; -.
DR AlphaFoldDB; Q96LA9; -.
DR SMR; Q96LA9; -.
DR BioGRID; 125577; 9.
DR STRING; 9606.ENSP00000314042; -.
DR ChEMBL; CHEMBL4523430; -.
DR GlyGen; Q96LA9; 2 sites.
DR BioMuta; MRGPRX4; -.
DR DMDM; 322510048; -.
DR PaxDb; Q96LA9; -.
DR PeptideAtlas; Q96LA9; -.
DR PRIDE; Q96LA9; -.
DR Antibodypedia; 24983; 120 antibodies from 25 providers.
DR DNASU; 117196; -.
DR Ensembl; ENST00000314254.3; ENSP00000314042.3; ENSG00000179817.5.
DR GeneID; 117196; -.
DR KEGG; hsa:117196; -.
DR MANE-Select; ENST00000314254.3; ENSP00000314042.3; NM_054032.3; NP_473373.2.
DR UCSC; uc001mnv.1; human.
DR CTD; 117196; -.
DR DisGeNET; 117196; -.
DR GeneCards; MRGPRX4; -.
DR HGNC; HGNC:17617; MRGPRX4.
DR HPA; ENSG00000179817; Not detected.
DR MIM; 607230; gene.
DR neXtProt; NX_Q96LA9; -.
DR OpenTargets; ENSG00000179817; -.
DR PharmGKB; PA142671337; -.
DR VEuPathDB; HostDB:ENSG00000179817; -.
DR eggNOG; ENOG502RTWA; Eukaryota.
DR GeneTree; ENSGT01030000234639; -.
DR InParanoid; Q96LA9; -.
DR OMA; LEWRFCD; -.
DR OrthoDB; 1123658at2759; -.
DR PhylomeDB; Q96LA9; -.
DR TreeFam; TF336336; -.
DR PathwayCommons; Q96LA9; -.
DR BioGRID-ORCS; 117196; 9 hits in 1021 CRISPR screens.
DR ChiTaRS; MRGPRX4; human.
DR GeneWiki; MRGPRX4; -.
DR GenomeRNAi; 117196; -.
DR Pharos; Q96LA9; Tbio.
DR PRO; PR:Q96LA9; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q96LA9; protein.
DR Bgee; ENSG00000179817; Expressed in upper leg skin and 2 other tissues.
DR Genevisible; Q96LA9; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR026234; MRGPCRFAMILY.
DR InterPro; IPR027344; MRGPCRX4.
DR PANTHER; PTHR11334; PTHR11334; 1.
DR PANTHER; PTHR11334:SF59; PTHR11334:SF59; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR02108; MRGPCRFAMILY.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..322
FT /note="Mas-related G-protein coupled receptor member X4"
FT /id="PRO_0000069783"
FT TOPO_DOM 1..31
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..60
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 82..96
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 118..137
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..177
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..218
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 240..254
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..322
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 299..322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 8
FT /note="F -> L (in dbSNP:rs2468774)"
FT /evidence="ECO:0000269|PubMed:11850634"
FT /id="VAR_019435"
FT VARIANT 25
FT /note="N -> K (in dbSNP:rs2445180)"
FT /evidence="ECO:0000269|PubMed:11850634"
FT /id="VAR_019436"
FT VARIANT 54
FT /note="Y -> C (in dbSNP:rs1869788)"
FT /evidence="ECO:0000269|PubMed:11850634"
FT /id="VAR_019437"
FT VARIANT 83
FT /note="L -> S (in dbSNP:rs2445179)"
FT /evidence="ECO:0000269|PubMed:11551509,
FT ECO:0000269|PubMed:11850634, ECO:0000269|PubMed:15489334"
FT /id="VAR_019438"
FT VARIANT 182
FT /note="A -> V (in dbSNP:rs11024532)"
FT /evidence="ECO:0000269|PubMed:11850634,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_025506"
FT VARIANT 245
FT /note="N -> S (in dbSNP:rs7102322)"
FT /id="VAR_049419"
FT CONFLICT 318
FT /note="S -> R (in Ref. 2; AAL86882)"
FT /evidence="ECO:0000305"
FT CONFLICT 319
FT /note="R -> K (in Ref. 2; AAL86883)"
FT /evidence="ECO:0000305"
FT HELIX 27..51
FT /evidence="ECO:0007829|PDB:7S8P"
FT TURN 52..55
FT /evidence="ECO:0007829|PDB:7S8P"
FT HELIX 62..85
FT /evidence="ECO:0007829|PDB:7S8P"
FT HELIX 92..109
FT /evidence="ECO:0007829|PDB:7S8P"
FT STRAND 110..112
FT /evidence="ECO:0007829|PDB:7S8P"
FT HELIX 113..125
FT /evidence="ECO:0007829|PDB:7S8P"
FT HELIX 127..132
FT /evidence="ECO:0007829|PDB:7S8P"
FT HELIX 138..154
FT /evidence="ECO:0007829|PDB:7S8P"
FT STRAND 157..159
FT /evidence="ECO:0007829|PDB:7S8P"
FT TURN 173..175
FT /evidence="ECO:0007829|PDB:7S8P"
FT HELIX 176..178
FT /evidence="ECO:0007829|PDB:7S8P"
FT HELIX 179..203
FT /evidence="ECO:0007829|PDB:7S8P"
FT STRAND 204..206
FT /evidence="ECO:0007829|PDB:7S8P"
FT HELIX 209..211
FT /evidence="ECO:0007829|PDB:7S8P"
FT HELIX 212..228
FT /evidence="ECO:0007829|PDB:7S8P"
FT HELIX 231..235
FT /evidence="ECO:0007829|PDB:7S8P"
FT TURN 239..241
FT /evidence="ECO:0007829|PDB:7S8P"
FT HELIX 249..266
FT /evidence="ECO:0007829|PDB:7S8P"
FT HELIX 268..272
FT /evidence="ECO:0007829|PDB:7S8P"
FT HELIX 274..279
FT /evidence="ECO:0007829|PDB:7S8P"
SQ SEQUENCE 322 AA; 36461 MW; A0D7B1ECD08FD15A CRC64;
MDPTVPVFGT KLTPINGREE TPCYNQTLSF TVLTCIISLV GLTGNAVVLW LLGYRMRRNA
VSIYILNLAA ADFLFLSFQI IRLPLRLINI SHLIRKILVS VMTFPYFTGL SMLSAISTER
CLSVLWPIWY RCRRPTHLSA VVCVLLWGLS LLFSMLEWRF CDFLFSGADS SWCETSDFIP
VAWLIFLCVV LCVSSLVLLV RILCGSRKMP LTRLYVTILL TVLVFLLCGL PFGILGALIY
RMHLNLEVLY CHVYLVCMSL SSLNSSANPI IYFFVGSFRQ RQNRQNLKLV LQRALQDKPE
VDKGEGQLPE ESLELSGSRL GP
