MRH4_LODEL
ID MRH4_LODEL Reviewed; 603 AA.
AC A5E5F7;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=ATP-dependent RNA helicase MRH4, mitochondrial;
DE EC=3.6.4.13;
DE Flags: Precursor;
GN Name=MRH4; ORFNames=LELG_04846;
OS Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC
OS 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade;
OC Lodderomyces.
OX NCBI_TaxID=379508;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 11503 / BCRC 21390 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL
RC YB-4239;
RX PubMed=19465905; DOI=10.1038/nature08064;
RA Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA Birren B.W., Kellis M., Cuomo C.A.;
RT "Evolution of pathogenicity and sexual reproduction in eight Candida
RT genomes.";
RL Nature 459:657-662(2009).
CC -!- FUNCTION: ATP-binding RNA helicase involved in mitochondrial RNA
CC metabolism. Required for maintenance of mitochondrial DNA (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. MRH4 subfamily.
CC {ECO:0000305}.
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DR EMBL; CH981530; EDK46665.1; -; Genomic_DNA.
DR RefSeq; XP_001524033.1; XM_001523983.1.
DR AlphaFoldDB; A5E5F7; -.
DR SMR; A5E5F7; -.
DR STRING; 379508.A5E5F7; -.
DR PRIDE; A5E5F7; -.
DR EnsemblFungi; EDK46665; EDK46665; LELG_04846.
DR GeneID; 5231117; -.
DR KEGG; lel:LELG_04846; -.
DR VEuPathDB; FungiDB:LELG_04846; -.
DR eggNOG; KOG0335; Eukaryota.
DR HOGENOM; CLU_003041_18_0_1; -.
DR InParanoid; A5E5F7; -.
DR OMA; FHALKMQ; -.
DR OrthoDB; 1223767at2759; -.
DR Proteomes; UP000001996; Unassembled WGS sequence.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; Mitochondrion; Nucleotide-binding;
KW Reference proteome; RNA-binding; Transit peptide.
FT TRANSIT 1..71
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 72..603
FT /note="ATP-dependent RNA helicase MRH4, mitochondrial"
FT /id="PRO_0000294673"
FT DOMAIN 193..409
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 443..603
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 151..158
FT /note="Q motif"
FT MOTIF 357..360
FT /note="DEAD box"
FT BINDING 206..213
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 603 AA; 67375 MW; 24FC0A62B4CEFB59 CRC64;
MISTGLPLFT LSKICHNCFF KQTRSLSRYS SKDKVKGRRR LLPRPNTEKF NSARSGVGVG
VGNGAGAGAR VGVGAGAGSV AAKVFESGNF SQLHNNGLSK QAQTAGLDLK QKITSFDQLK
VFPSVREAMI KEIKSQYNLK GPQHSSIDDV VIKPTPVQIA AIRKINQTRK IKVAKNLDEM
SEGERIQIEL QTKNEEQKTK IFTVAAETGS GKTWAYLANI MSKLKEDDYK WFNQSPEAYN
SFKSSEQVRS VILLPTHELV EQVYETLKRA NSFLLEYENV PLQYKEFLNL PEHHTLGLSI
AKLSHGDAPI NVYKQLRNRG KIDILITTPG KITSFSKLES IDRPFKIFKS IRYCVIDEAD
TLFDDSFLKD TTAVVKNFPK LLDLILVSAT IPKEFEKTLL RLFPDQKSLI RVATPSLHKI
SKNIKVMTLD ADLAPYNGSK TRCLAQAIYA ISKDGTEHNH VKRIIIFVNE KAEVDGLVDL
LQSKYHIRRE DICGISGSVN VGDRKDYLEP FLKPAQLLED DVDQSKIKIL VTTDLLARGM
NFIGIKNVIL MGLPKSSVEL VHRLGRTGRM NQLGRVFIIV DKKSRKSWVK GLGSAIMKGS
RIG
