ID   MRJ1_CRYNH              Reviewed;         331 AA.
AC   J9VP29;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   02-DEC-2020, sequence version 2.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=Mitochondrial respiration co-chaperone MRJ1 {ECO:0000305};
DE   AltName: Full=Mitochondrial respiration J-domain protein 1 {ECO:0000303|PubMed:32518190};
DE   Flags: Precursor;
GN   Name=MRJ1 {ECO:0000303|PubMed:32518190};
GN   ORFNames=CNAG_00938 {ECO:0000312|EMBL:AFR95191.1};
OS   Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 /
OS   CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=235443 {ECO:0000312|Proteomes:UP000010091};
RN   [1] {ECO:0000312|Proteomes:UP000010091}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H99 / ATCC 208821 / CBS 10515 / FGSC 9487;
RX   PubMed=24743168; DOI=10.1371/journal.pgen.1004261;
RA   Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V.,
RA   Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F.,
RA   Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A.,
RA   Gaillardin C., Gerik K.J., Goldberg J., Gonzalez-Hilarion S., Gujja S.,
RA   Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L.,
RA   Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K.,
RA   Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A.,
RA   Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A.,
RA   Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A.,
RA   Cuomo C.A., Dietrich F.S.;
RT   "Analysis of the genome and transcriptome of Cryptococcus neoformans var.
RT   grubii reveals complex RNA expression and microevolution leading to
RT   virulence attenuation.";
RL   PLoS Genet. 10:E1004261-E1004261(2014).
RN   [2] {ECO:0000305}
RP   IDENTIFICATION OF N-TERMINUS, FUNCTION, INTERACTION WITH QCR2, SUBCELLULAR
RP   LOCATION, INDUCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF HIS-111.
RX   PubMed=32518190; DOI=10.1128/mbio.01127-20;
RA   Horianopoulos L.C., Hu G., Caza M., Schmitt K., Overby P., Johnson J.D.,
RA   Valerius O., Braus G.H., Kronstad J.W.;
RT   "The Novel J-Domain Protein Mrj1 Is Required for Mitochondrial Respiration
RT   and Virulence in Cryptococcus neoformans.";
RL   MBio 11:E01127-E01127(2020).
CC   -!- FUNCTION: Mitochondrial co-chaperone required for ubiquinol-cytochrome
CC       c oxidoreductase (mitochondrial respiratory chain complex III)
CC       activity. {ECO:0000269|PubMed:32518190}.
CC   -!- SUBUNIT: Interacts with QCR2. {ECO:0000269|PubMed:32518190}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:32518190}.
CC   -!- INDUCTION: Induced by high temperature (at protein level).
CC       {ECO:0000269|PubMed:32518190}.
CC   -!- DISRUPTION PHENOTYPE: Decreases electron flow through the mitochondrial
CC       respiratory chain complex III (PubMed:32518190). Increases level of
CC       depolarized mitochondria (PubMed:32518190). Abnormal cell wall
CC       structure resulting in abnormal capsule attachment (PubMed:32518190).
CC       Sensitive to iron starvation (PubMed:32518190). Abolishes cell
CC       population growth on non-fermentable carbon sources (glycerol, lactate,
CC       and succinate) (PubMed:32518190). Decreases cell population growth
CC       (PubMed:32518190). Decreases virulence in a mouse intranasal model of
CC       infection (PubMed:32518190). {ECO:0000269|PubMed:32518190}.
CC   -!- SIMILARITY: Belongs to the DnaJ family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AFR95191.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP003824; AFR95191.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_012049209.1; XM_012193819.1.
DR   AlphaFoldDB; J9VP29; -.
DR   SMR; J9VP29; -.
DR   EnsemblFungi; AFR95191; AFR95191; CNAG_00938.
DR   GeneID; 23884705; -.
DR   VEuPathDB; FungiDB:CNAG_00938; -.
DR   HOGENOM; CLU_1133539_0_0_1; -.
DR   Proteomes; UP000010091; Chromosome 5.
DR   GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0006122; P:mitochondrial electron transport, ubiquinol to cytochrome c; IMP:UniProtKB.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR   InterPro; IPR036869; J_dom_sf.
DR   Pfam; PF00226; DnaJ; 1.
DR   PRINTS; PR00625; JDOMAIN.
DR   SMART; SM00271; DnaJ; 1.
DR   SUPFAM; SSF46565; SSF46565; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
DR   PROSITE; PS50096; IQ; 1.
PE   1: Evidence at protein level;
KW   Chaperone; Electron transport; Mitochondrion; Respiratory chain;
KW   Transit peptide; Transport.
FT   TRANSIT         1..36
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           37..331
FT                   /note="Mitochondrial respiration co-chaperone MRJ1"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000451743"
FT   DOMAIN          83..147
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT   DOMAIN          275..303
FT                   /note="IQ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT   REGION          35..66
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          203..226
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        35..52
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         111
FT                   /note="H->Q: Decreases cell population growth rate."
FT                   /evidence="ECO:0000269|PubMed:32518190"
SQ   SEQUENCE   331 AA;  36438 MW;  A76AE00DA083D077 CRC64;
     MLSFQATVRP LAVSSRLHSP AAHIWRRNAH TAAMSDDSLD QGSSSSYGDS ASQPHLGKGK
     GRQDSLAQSY RFPEKGMNGG PPDPFEVMAL DRSATQQEVK QQYYKLALLL HPDSSHPSSS
     PDHFATLNKA YNLLSKQSSR SAFLKTGYGW DVSTSSGGNQ TWSDSLMRAE IARRRNGGAA
     AWNGASRRYR DSDAGRGAWG GFDGSQGWRP YEDPSKGFSP PTSGPAEERY MSNPRFLAVV
     GVASAVIAWV HWHRLGYAAE THRDMLDKQN IDASRALAQA RYEAATHGHI RREQIRRRVR
     EAEVLKELEK ADQGHIAVAG PPTAYPPSHR E