ID   MRJP4_APIME             Reviewed;         464 AA.
AC   Q17061;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Major royal jelly protein 4;
DE            Short=MRJP-4;
DE   AltName: Full=Bee-milk protein;
DE   AltName: Full=Royal jelly protein RJP57-2;
DE   Flags: Precursor;
GN   Name=MRJP4;
OS   Apis mellifera (Honeybee).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC   Apis.
OX   NCBI_TaxID=7460;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Head;
RX   AGRICOLA=IND20437271;
RA   Klaudiny J., Hanes J., Kulifajova J., Albert S., Simuth J.;
RT   "Molecular cloning of two cDNAs from the head of the nurse honey bee (Apis
RT   mellifera L.) for coding related proteins of royal jelly.";
RL   J. Apic. Res. 33:105-111(1994).
RN   [2]
RP   CHARACTERIZATION.
RC   TISSUE=Head;
RX   PubMed=9791542; DOI=10.1007/s000180050229;
RA   Schmitzova J., Klaudiny J., Albert S., Schroeder W., Schreckengost W.,
RA   Hanes J., Judova J., Simuth J.;
RT   "A family of major royal jelly proteins of the honeybee Apis mellifera L.";
RL   Cell. Mol. Life Sci. 54:1020-1030(1998).
CC   -!- FUNCTION: May play an important role in honeybee nutrition. It is found
CC       in the royal jelly which is the food of the queen honey bee larva. The
CC       royal jelly determines the development of the young larvae and is
CC       responsible for the high reproductive ability of the honeybee queen.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Hypopharyngeal glands of nurse honey bees.
CC   -!- DEVELOPMENTAL STAGE: Produced by the cephalic glandular system of the
CC       nurse honey bee.
CC   -!- SIMILARITY: Belongs to the major royal jelly protein family.
CC       {ECO:0000305}.
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DR   EMBL; Z26319; CAA81228.1; -; mRNA.
DR   PIR; S39194; S39194.
DR   RefSeq; NP_001011610.1; NM_001011610.1.
DR   AlphaFoldDB; Q17061; -.
DR   SMR; Q17061; -.
DR   STRING; 7460.GB55206-PA; -.
DR   PaxDb; Q17061; -.
DR   EnsemblMetazoa; NM_001011610; NP_001011610; GeneID_406133.
DR   GeneID; 406133; -.
DR   KEGG; ame:406133; -.
DR   CTD; 406133; -.
DR   eggNOG; ENOG502SCJK; Eukaryota.
DR   InParanoid; Q17061; -.
DR   PhylomeDB; Q17061; -.
DR   Proteomes; UP000005203; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.120.10.30; -; 1.
DR   InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR   InterPro; IPR017996; Royal_jelly/protein_yellow.
DR   PANTHER; PTHR10009; PTHR10009; 1.
DR   Pfam; PF03022; MRJP; 1.
DR   PRINTS; PR01366; ROYALJELLY.
PE   1: Evidence at protein level;
KW   Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   CHAIN           16..464
FT                   /note="Major royal jelly protein 4"
FT                   /id="PRO_0000031046"
FT   REGION          419..464
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        435..451
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        26
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        31
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        79
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        149
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        165
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        242
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        322
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        368
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   464 AA;  52915 MW;  10AE07BCC10E1265 CRC64;
     MTKWLLLMVC LGIACQNIRG GVVRENSSGK NLTNTLNVIH KWKYLDYDFD NDERRQAAIQ
     SGEYDRTKNY PLDVDQWHNK TFLAVIRYNG VPSSLNVVSD KTGNGGRLLQ PYPDWSFAKY
     EDCSGIVSAH KIAIDEYERL WVLDSGLVNN TQPMCSPKLF AFDLNTSQLL KQVEIPHDVA
     TTGKGELVSL TVQAMDSTNT MVYMVDNKNT LIIYQNADDS FHRLSSHTLN HNSDKMSDQQ
     ENLTLKEVDN KVYGMALSPV THNLYYNSPS SENLYYVNTE SLMKSENQGN DVQYERVQDV
     FDSQLTVKAV SKNGVLLFGL ANNTLSCWNE HQSLDRQNID VVARNEDTLQ MVVSMKIKQN
     VPQSGRVNNT QRNEYLLALS DRNQNVLNND LNLEHVNFQI LGANVNDLIR NSRCANFDNQ
     DNNHYNHNHN QARHSSKSDN QNNNQHNDQA HHSSKSNNRH NNND